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The storage cell walls in the endosperm of Asparagus officinalis L. seeds during development and following germination

Published online by Cambridge University Press:  22 February 2007

Heather A. Williams ,
J. Derek Bewley ,
John S. Greenwood ,
Richard Bourgault  and
Beixin Mo
Heather A. Williams
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J. Derek Bewley*
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
John S. Greenwood
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Richard Bourgault
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Beixin Mo
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
*
*Correspondence Tel: (519) 824-4120 Ext. 4451 Fax: (519) 767-1991 Email: dbewley@uoguelph.ca
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Abstract

The thickened cell walls of endosperm cells of asparagus (Asparagus officinalis) seeds are composed of glucomannans. During development, the extensive thickening of the cell wall is specific to the lateral region of the endosperm and not to the micropylar region. The micropylar endosperm may thus be predisposed to facilitate germination. Following germination there is a progressive mobilization of the reserves in the cells of the lateral endosperm in a wave-like manner away from the haustorial cotyledon, accompanied by the loss of cytoplasm and the crushing of the lateral endosperm cells. Although the internal reserves are mobilized from the micropylar endosperm cells, the cells themselves remain intact throughout the period of lateral endosperm mobilization and may form a living barrier to prevent the loss of soluble hydrolysis products of the lateral endosperm to the surrounding environment. The location and timing of endo-β-mannanase production and the increase in activity of β-mannoside mannohydrolase in seeds of germinated asparagus were followed. Endo-β-mannanase activity increases greatly in the endosperm until the mid-point of mobilization, and is about 45 times higher than in the embryo on a per seed part basis. Unlike endo-β-mannanase, which is extractable in low-salt buffer, β-mannoside mannohydrolase requires high salt (0.5 M NaCl) for extraction. This enzyme continually increases in activity in both the endosperm and embryo following germination, with the majority of the activity being concentrated in the embryo when considered on a per seed part basis.

Keywords

endo- β-mannanaseβ-mannoside manno-hydrolaseendosperm cell wallsAsparagus officinalis Lstorage reservesreserve mobilization
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 4 , December 2001 , pp. 305 - 315
Copyright
Copyright © Cambridge University Press 2001

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References

Bewley, J.D. (1997) Breaking down the walls—a role for endo-β-mannanase in release from seed dormancy? Trends in Plant Science 2, 464–469.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination (2nd edition). New York, Plenum Press.CrossRefGoogle Scholar
Bewley, J.D. and Reid, J.S.G. (1985) Mannans and glucomannans. pp. 289–304 in Dey, P.M.; Dixon, R.A. (Eds) Biochemistry of storage carbohydrates in green plants. London, Academic Press.Google Scholar
Bewley, J.D., Banik, M., Bourgault, R., Feurtado, J.A., Toorop, P. and Hilhorst, H.W.M. (2000) Endo-β mannanase activity in the skin and outer pericarp of tomato fruits during ripening. Journal of Experimental Botany 51, 529–538.CrossRefGoogle ScholarPubMed
Bourgault, R., Bewley, J.D., Alberici, A. and Decker, D. (2001) Endo-β-mannanase activity in tomato and other ripening fruits. HortScience 36, 72–75.CrossRefGoogle Scholar
Brown, M.J. and Greenwood, J.S. (1990) Involvement of the Golgi apparatus in crystalloid protein deposition in Ricinus communis cv. Hale seeds. Canadian Journal of Botany 68, 2353–2360.CrossRefGoogle Scholar
DeMason, D.A., Sexton, R. and Reid, J.S.G. (1983) Structure, composition and physiological state of the endosperm of Phoenix dactylifera L. Annals of Botany 52, 71–80.CrossRefGoogle Scholar
Dirk, L.M.A., Griffen, A.M., Downie, B. and Bewley, J.D. (1995) Multiple isozymes of endo-β-mannanase in dry and imbibed seeds. Phytochemistry 40, 1045–1056.CrossRefGoogle Scholar
Downie, B., Hilhorst, H.W.M. and Bewley, J.D. (1994) A new assay for quantifying endo-β-D-mannanase activity using Congo Red dye. Phytochemistry 36, 829–835.CrossRefGoogle Scholar
Downie, B., Hilhorst, H.W.M. and Bewley, J.D. (1997) Endo-β-mannanase activity during dormancy alleviation and germination of white spruce (Picea glauca) seeds. Physiologia Plantarum 101, 405–415.CrossRefGoogle Scholar
Dubreucq, B., Berger, N., Vincent, E., Boisson, M., Pelletier, G., Caboche, M. and Lepiniec, L. (2000) The Arabidopsis AtEPR1 extensin-like gene is specifically expressed in endosperm during seed germination. The Plant Journal 23, 643–652.CrossRefGoogle ScholarPubMed
Dwarte, D. and Ashford, A.E. (1982) The chemistry and microstructure of protein bodies in celery endosperm. Botanical Gazette 143, 164–175.CrossRefGoogle Scholar
Goldberg, R. and Roland, J.C. (1971) Étude de l'utilization des glucomannanes au cours de la germination des graines d'Asparagus officinalis. Révue Générale Botanique 78, 75–102.Google Scholar
Goldberg, R., Guillou, L., Prat, R., Du Penhoat, C.H. and Michon, V. (1991) Structural features of the cell-wall polysaccharides of Asparagus officinalis seeds. Carbohydrate Research 210, 263–276.CrossRefGoogle ScholarPubMed
Goldberg, R., Guillou, L., Prat, R., Dubacq, J.-P. and Roland, J.C. (1992) Structure and biochemistry of endosperm breakdown in Asparagus officinalis seedlings. Plant Physiology and Biochemistry 30, 477–485.Google Scholar
Groot, S.P.C., Kieliszewska-Rockika, B., Vermeer, E. and Karssen, C.M. (1988) Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion. Planta 174, 500–504.CrossRefGoogle ScholarPubMed
Halmer, P. and Bewley, J.D. (1979) Mannanase production by the lettuce endosperm. Control by the embryo. Planta 144, 333–340.CrossRefGoogle ScholarPubMed
Jacobsen, J.V. and Pressman, E. (1979) A structural study of germination in celery (Apium graveolens L.) seed with emphasis on endosperm breakdown. Planta 144, 241–248.CrossRefGoogle Scholar
Kontos, F., Spyropoulos, C.G., Griffen, A. and Bewley, J.D. (1996) Factors affecting endo-β-mannanase activity in the endosperms of fenugreek and carob seeds. Seed Science Research 6, 23–29.CrossRefGoogle Scholar
Malek, L. and Bewley, J.D. (1991) Endo-β-mannanase activity and reserve mobilization in excised endosperms of fenugreek is affected by volume of incubation and abscisic acid. Seed Science Research 1, 45–49.CrossRefGoogle Scholar
McClendon, J.H., Nolan, W.G. and Wenzler, H.F. (1976) The role of the endosperm in the germination of legumes: Galactomannan, nitrogen, and phosphorus changes in the germination of guar (Cyamopsis tetragonoloba: Leguminosae). American Journal of Botany 63, 790–797.CrossRefGoogle Scholar
Ouellette, B.F.F. and Bewley, J.D. (1986) β-Mannoside mannohydrolase and the mobilization of the endosperm cell wall of lettuce seeds, cv. Grand Rapids. Planta 169, 333–338.CrossRefGoogle ScholarPubMed
Reid, J.S.G.. (1985) Galactomannans. pp.265–288in Dey, P.M., Dixon, R.A. (Eds) Biochemistry of storage carbohydrates in green plants. London, Academic Press.Google Scholar
Reid, J.S.G. and Bewley, J.D. (1979) A dual role for the endosperm and its galactomannan reserves in the germination physiology of fenugreek (Trigonella foenumgraecum L.), an endospermic leguminous seed. Planta 147, 145–150.CrossRefGoogle Scholar
Reid, J.S.G. and Meier, H. (1972) The function of the aleurone layer during galactomannan mobilisation in germinating seeds of fenugreek (Trigonella foenumgraecum L.), crimson clover (Trifolium incarnatum L.), and lucerne (Medicago sativa L.): A correlative biochemical and ultrastructural study. Planta 106, 44–60.CrossRefGoogle ScholarPubMed
Reid, J.S.G. and Meier, H. (1973) Enzymic activities and galactomannan mobilisation in germinating seeds of fenugreek (Trigonella foenum-graecum L., Leguminosae). Secretion of αgalactosidase and β-mannosidase by the aleurone layer. Planta 112, 301–308.CrossRefGoogle Scholar
Sanchez, R.A. and de Miguel, L. (1997) Phytochrome promotion of mannan-degrading enzyme activities in the micropylar endosperm of Datura ferox seeds requires the presence of the embryo and gibberellin synthesis. Seed Science Research 7, 27–33.CrossRefGoogle Scholar
Seiler, A. (1977) Galaktomannanabbau in keimender Johannisbrotsamen (Ceratonia siliqua L.). Planta 134, 209–221.CrossRefGoogle Scholar
Toorop, P.E., Bewley, J.D. and Hilhorst, H.W.M. (1996) Endo-β-mannanase isoforms are present in the endosperm and embryo of tomato seeds, but are not essentially linked to the completion of germination. Planta 200, 153–158.CrossRefGoogle Scholar