Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-10T11:41:19.820Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of QTLs for the micromorphology on the seed coat surface of soybean using recombinant inbred lines

Published online by Cambridge University Press:  21 September 2015

Kazunori Otobe ,
Satoshi Watanabe  and
Kyuya Harada
Kazunori Otobe*
Affiliation:
NARO Agricultural Research Center, Kannondai, Tsukuba3-1-1, Ibaraki305-8666, Japan
Satoshi Watanabe
Affiliation:
Faculty of Agriculture Saga University, Honjo-machi 1, Saga 840-8502, Japan
Kyuya Harada
Affiliation:
National Institute of Agrobiological Sciences, Tsukuba 2-1-2, Ibaraki, 305-8602, Japan
*
*Correspondence E-mail: otobek@affrc.go.jp
Get access Rights & Permissions [Opens in a new window]

Abstract

The seed coat of soybean (Glycine max (L.) Merrill) must protect the seed but allow water intake. Overprotection, causing impermeability, is assumed to be due to the presence of an impermeable layer in the seed coat, although validation of this assumption has relied on imbibition testing, which tends to be influenced by microfractures in the seed coat. Recent micromorphological analyses using laser-assisted topography microscopy revealed links to the surface roughness (SR) of the seed coat. To verify genetic links between hardseededness and SR, we analysed quantitative trait loci (QTLs) governing SR formation using 148 recombinant inbred lines (RILs) with a genetic linkage map covering 2663.6 cM of all 20 linkage groups of soybean, with 355 DNA markers and 5 phenotype markers. Five QTLs were detected, including previously identified hardseededness QTLs for ratio of seeds absorbing water, namely RAS1 and RAS2, which accounted for 20% of the phenotypic variance, and one near a locus inhibiting seed coat colour (I). These results indicate that the impermeability of soybean seed is genetically related to the reduction of SR.

Keywords

Glycine max (L.) Merrilllinkage mapmicromorphologyquantitative trait locirecombinant inbred linesseed coattopography microscope
Type
Research Papers
Information
Seed Science Research , Volume 25 , Issue 4 , December 2015 , pp. 409 - 415
Check for updates
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Argel, P.J. and Paton, C.J. (1999) Overcoming legume hardseededness in forage seed production. pp. 247–259 in Loch, D.S.; Ferguson, J.E. (Eds) Tropical and subtropical species. Vol.2. Wallingford, UK, CAB International.Google Scholar
Baskin, C.C. and Baskin, J.M. (2014) Seeds: Ecology, biogeography and evolution of dormancy and germination (2nd edition). New York, Academic Press.Google Scholar
Baskin, J.M., Baskin, C.C. and Li, X. (2000) Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biology 15, 139–152.Google Scholar
Broman, K.W., Wu, H., Sen, Ś. and Churchill, G.A. (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19, 889–890.Google Scholar
Calero, E., West, S.H. and Hinson, K. (1981) Water absorption of soybean seeds and associated causal factors. Crop Science 21, 926–933.CrossRefGoogle Scholar
Chachalis, D. and Smith, M.L. (2001) Seed coat regulation of water uptake during imbibition in soybeans. Glycine max (L.) Merr.). Seed Science and Technology 29, 401–412.Google Scholar
Hartwig, E.E. and Potts, H.C. (1987) Development and evolution of impermeable seed coats for preserving soybean seed quality. Crop Science 27, 506–508.Google Scholar
Heatherly, L.G., Kenty, M.M. and Kilen, T.C. (1995) Effects of storage environment and duration on impermeable seed coat in soybean. Field Crops Research 40, 57–62.Google Scholar
Kebede, H., Smith, J.R. and Ray, J.D. (2014) Identification of a single gene for seed coat impermeability in soybean PI 594619. Theoretical and Applied Genetics 127, 1991–2003.Google Scholar
Keim, P., Diers, B.W. and Shoemaker, R.C. (1990) Genetic analysis of soybean hard seededness with molecular markers. Theoretical and Applied Genetics 79, 465–469.Google Scholar
Kilen, T.C. and Hartwig, E.E. (1978) An inheritance study of impermeable seed in soybeans. Field Crops Research 1, 65–70.Google Scholar
Koizumi, M., Kikuchi, K., Isobe, S., Ishida, N., Naito, S. and Kano, H. (2008) Role of seed coat in imbibing soybean seeds observed by micro-magnetic resonance imaging. Annals of Botany 102, 343–352.Google Scholar
Kulik, M.M. and Yaklich, R.W. (1991) Soybean seed coat structures; relationship to weathering resistance and infection by fungus Phomopsis phaseoli . Crop Science 31, 108–113.Google Scholar
Liu, B., Fujita, T., Yan, Z.-H., Sakamoto, S., Xu, D. and Abe, J. (2007) QTL mapping of domestication-related traits in soybean (Glycine max). Annals of Botany 100, 1027–1038.Google Scholar
Ma, F., Cholewa, E., Mohamed, T., Peterson, C.A. and Gijzen, M. (2004) Cracks in the palisade cuticle of soybean seed coats correlate with their permeability to water. Annals of Botany 94, 213–228.Google Scholar
Marbach, I. and Mayer, A.M. (1974) Permeability of seed coats to water as related to drying conditions and metabolism of phenolics. Plant Physiology 54, 817–820.Google Scholar
Meyer, C.J., Steudle, E. and Peterson, C.A. (2007) Patterns and kinetics of water uptake by soybean seeds. Journal of Experimental Botany 58, 717–732.CrossRefGoogle ScholarPubMed
Mullin, W.J. and Xu, W. (2001) Study of soybean seed coat components and their relationship to water absorption. Journal of Agricultural and Food Chemistry 49, 5331–5335.Google Scholar
Otobe, K. and Yoshioka, K. (2008) Property of the surface configuration on the impermeable seed coat. Japanese Journal of Crop Science 77, 69–77 (in Japanese).Google Scholar
Qutob, D., Ma, F., Peterson, C.A., Bernards, M.A. and Gijzen, M. (2008) Structural and permeability properties of the soybean seed coat. Botany 86, 219–227.Google Scholar
Roy, K.W., Keith, B.C. and Andrews, C.H. (1994) Resistance of hardseeded soybean lines to seed infection by Phomopsis, other fungi, and soybean mosaic virus. Canadian Journal of Plant Pathology 16, 122–128.Google Scholar
Sakamoto, S., Abe, J., Kanazawa, A. and Shimamoto, Y. (2004) Marker-assisted analysis for soybean hard seededness with isozyme and simple sequence repeat loci. Breeding Science 54, 133–139.Google Scholar
Scala, N.L. Jr, Florentino, A.O. and Carvalho, N.M. (1999) Determination of pore size distribution in soybean seed coats by mercury porosimetry. Seed Science and Technology 27, 371–376.Google Scholar
Senda, M., Kurauchi, T., Kasai, A. and Ohnishi, S. (2012) Suppressive mechanism of seed coat pigmentation in yellow soybean. Breeding Science 61, 523–530.Google Scholar
Shao, S., Meyer, C.J., Ma, F., Peterson, C.A. and Bernards, M.A. (2007) The outermost cuticle of soybean seeds: chemical composition and function during imbibition. Journal of Experimental Botany 58, 1071–1082.Google Scholar
Smýkal, P., Vernoud, V., Blair, M.W., Soukup, A. and Thompson, R.D. (2014) The role of the testa during development and in establishment of dormancy of the legume seed. Frontiers in Plant Science 5, 351.Google Scholar
Song, Q.J., Marek, L.F., Shoemaker, R.C., Lark, K.G., Concibido, V.C., Delannay, X., Specht, J.E. and Cregan, P.B. (2004) A new integrated genetic linkage map of the soybean. Theoretical and Applied Genetics 109, 122–128.Google Scholar
Tyler, J.M. (1997) Effect of impermeable seed coat on germination of seed from early maturing soybean. Seed Technology 19, 45–50.Google Scholar
Watanabe, S., Tajuddin, T., Yamanaka, N., Hayashi, M. and Harada, K. (2004) Analysis of QTLs for reproductive development and seed quality traits in soybean using recombinant inbred lines. Breeding Science 54, 399–407.Google Scholar
Yaklich, R.W., Vigil, E.I. and Wergin, W.P. (1984) Scanning electron microscopy of soybean seed coat. Scanning Electron Microscopy II, 991–1000.Google Scholar
Yaklich, R.W., Vigil, E.I. and Wergin, W.P. (1986) Pore development and seed coat permeability in soybean. Crop Science 26, 616–624.Google Scholar
Supplementary material: File

Otobe supplementary material

Figure S1

Download Otobe supplementary material(File)
File 36.3 KB
Supplementary material: File

Otobe supplementary material

Table S1

Download Otobe supplementary material(File)
File 27.2 KB