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Biochemical changes associated with gibberellic acid-like activity of smoke-water, karrikinolide and vermicompost leachate during seedling development of Phaseolus vulgaris L.

Published online by Cambridge University Press:  17 February 2014

Satendra Singh ,
Manoj G. Kulkarni  and
Johannes Van Staden
Satendra Singh
Affiliation:
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa
Manoj G. Kulkarni
Affiliation:
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa
Johannes Van Staden*
Affiliation:
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa
*
*Correspondence E-mail: rcpgd@ukzn.ac.za
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Abstract

Smoke-water (SW), karrikinolide (KAR1) and vermicompost leachate (VCL) have been reported to possess gibberellic acid-like activity. The effects of these plant growth-promoting substances (PGPSs) on biochemical changes occurring during seed germination of Phaseolus vulgaris were assessed. Seeds were incubated/germinated under dark conditions in water (control) or with different concentrations of SW, KAR1, VCL and gibberellic acid (GA3) for 7 d. The maximum seedling fresh weight (1.863 g) was recorded for the SW (1:750 v/v) treatment. The longest seedling axes (9.9 cm) and the highest number of adventitious roots (16.3) were recorded for VCL-treated seedlings (1:10 and 1:5 v/v, respectively). Analysis of two important hydrolytic enzymes, acid phosphatase and alpha-amylase, showed maximum activity [1856 nkat mg− 1 and 3.225 mg min− 1(g FW)− 1, respectively] in the seeds incubated with 10− 8M KAR1. In all the treated seedlings, proline content was significantly reduced [43.67 μg (g FW)− 1; VCL (1:20 v/v)] in comparison to the control [87 μg (g FW)− 1] but there was an increase in amino acids with some concentrations of PGPSs. The tested PGPSs significantly influenced various biochemical parameters that play a significant role in seed germination and plant growth. This study indicates that PGPSs may act via stress-relieving biochemical pathways during seed germination.

Keywords

beangibberellic acidseedling axissmoke-watervermicompost leachate
Type
Research Papers
Information
Seed Science Research , Volume 24 , Issue 1 , March 2014 , pp. 63 - 70
Copyright
Copyright © Cambridge University Press 2014 

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