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Delayed germination of Brassica parachinensis seeds by coumarin involves decreased GA4 production and a consequent reduction of ROS accumulation

Published online by Cambridge University Press:  10 August 2021

Bing-Xian Chen Open the ORCID record for Bing-Xian Chen [Opens in a new window] ,
Yuan-Xuan Peng ,
Xue-Qin Yang  and
Jun Liu
Bing-Xian Chen
Affiliation:
Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservationand Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China
Yuan-Xuan Peng
Affiliation:
Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservationand Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, China
Xue-Qin Yang
Affiliation:
College of Life Sciences, South China Agricultural University, Guangzhou, China
Jun Liu*
Affiliation:
Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservationand Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China
*
*Author for Correspondence: Jun Liu, E-mail: liujun139@139.com
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Abstract

The plant allelochemical coumarin effectively inhibits the germination of Brassica parachinensis (B. parachinensis) seeds. Quantification of endogenous phytohormones showed that contents of abscisic acid (ABA), ABA glucose ester, gibberellin A20 (GA20), GA3, GA15, GA24, GA9 and GA4 were higher in germinating seeds than in seedlings. Moreover, the presence of coumarin significantly reduced the content of bioactive GA4 which is thought to positively regulate seed germination. Histochemical staining and spectrophotometry of reactive oxygen species (ROS) revealed that exogenous GA3 and GA4+7 could effectively promote the production of endogenous ROS during germination and that the GA synthesis inhibitor paclobutrazol could effectively inhibit production of ROS. Coumarin significantly inhibited the accumulation of ROS, especially superoxide anion radical (${\rm O}_2^{{\cdot}{-}} $). This inhibitory effect could be restored by the addition of exogenous GA3 and GA4+7. Coumarin also inhibited the activity of the ROS-degrading enzymes such as superoxide dismutase, catalase and peroxidase as well as β-amylase in seeds and seedlings. Taken together, we propose a model for the regulation of seed germination in B. parachinensis by coumarin, Gas and ROS, in which coumarin may delay seed germination by reducing endogenous GA4, thus decreasing the accumulation of ROS.

Keywords

coumarinGAsROSseed germination
Type
Research Paper
Information
Seed Science Research , Volume 31 , Issue 3 , September 2021 , pp. 224 - 235
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

†

These authors contributed equally to this study.

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