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Does benzyladenine stimulate DNA metabolism by modifying gene expression during maize germination?

Published online by Cambridge University Press:  19 September 2008

José Juan Zúñiga-Aguilar ,
Imelda López ,
Alberto Gómez  and
Jorge M. Vázquez-Ramos
José Juan Zúñiga-Aguilar
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
Imelda López
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
Alberto Gómez
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
Jorge M. Vázquez-Ramos*
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México 04510, D.F., México
*
*Correspondence
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Abstract

Benzyladenine, a synthetic cytokinin, stimulates DNA metabolism during maize germination; apparently such stimulation occurs in two phases, and during the first phase stimulations of DNA synthesis, of protein kinase activity (both α-amanitin repressible), of DNA polymerase and poly ADP-ribosyl polymerase activities are observed. Since the evidence suggested that the effects produced by BA were due, at least partially, to transcriptional activation, attempts were made to discover genes, by subtractive hybridization of a cDNA library or by differential display, whose expression depended on the presence of BA; however these were unsuccessful. BA modulated the expression of a limited number of genes only. The possible mechanism by which BA could be promoting DNA metabolism during maize germination is discussed.

Keywords

maizeseed germinationbenzyladenineDNA metabolism
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 5 , Issue 4 , December 1995 , pp. 219 - 226
Copyright
Copyright © Cambridge University Press 1995

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