Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-10T12:31:25.424Z Has data issue: false hasContentIssue false
  • English
  • Français

Galactosyl-sucrose oligosaccharides and potential longevity of primed seeds

Published online by Cambridge University Press:  22 February 2007

Sunitha Gurusinghe  and
Kent J. Bradford
Sunitha Gurusinghe
Affiliation:
Department of Vegetable Crops, One Shields Avenue, University of California, Davis, CA 95616-8631, USA
Kent J. Bradford*
Affiliation:
Department of Vegetable Crops, One Shields Avenue, University of California, Davis, CA 95616-8631, USA
*
*Correspondence Fax: 1-530-752-4554 Email: kjbradford@ucdavis.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Correlative evidence indicates that sucrose and α-galactosyl-sucrose oligosaccharides (raffinose family oligosaccharides; RFOs) may be involved in seed longevity. Priming treatments (hydration in water or osmotic solutions followed by drying) can improve short-term seed performance but often result in reduced seed longevity. As RFOs are metabolized quickly following seed imbibition, loss of RFOs during priming could lead to more rapid deterioration in dry storage. This hypothesis was tested by measuring sucrose and oligosaccharide contents and potential longevity of primed seeds. Raffinose contents of whole lettuce (Lactuca sativa L.) seeds declined during hydration and priming and were correlated with decreased median potential viability (p50). However, this relationship was less significant when only the embryonic axes were analysed. In tomato (Lycopersicon esculentum Mill.) and impatiens (Impatiens balsamina L.) seeds, planteose was the major galactosyl-sucrose oligosaccharide and only small quantities of RFOs were present. Planteose contents declined during priming in seeds of both species, while sucrose contents increased or remained constant. Post-priming treatments that restored longevity in primed impatiens and tomato seeds were not accompanied by consistent changes in RFO or planteose contents. Our data do not rule out a role for oligosaccharides in seed longevity, but they make it unlikely that changes in oligosaccharide contents alone are responsible for the reduction in longevity due to priming or its restoration by post-priming treatments.

Keywords

impatiensImpatiens balsamina LlettuceLactuca sativa Loligosaccharidesplanteoseraffinoseseed longevityseed primingtomatoLycopersicon esculentum Mill
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 2 , June 2001 , pp. 121 - 134
Copyright
Copyright © Cambridge University Press 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Amuti, K.S. and Pollard, C.J. (1977) Soluble carbohydrates of dry and developing seeds. Phytochemistry 16, 529–532.CrossRefGoogle Scholar
Argerich, C.A. and Bradford, K.J. (1989) The effects of priming and aging on seed vigor in tomato. Journal of Experimental Botany 40, 599–607.CrossRefGoogle Scholar
Bernal-Lugo, I. and Leopold, A.C. (1995) Seed stability during storage: raffinose content and seed glassy state. Seed Science Research 5, 75–80.CrossRefGoogle Scholar
Brenac, P., Horbowicz, M., Downer, S.M., Dickerman, A.M., Smith, M.E. and Obendorf, R.L. (1997) Raffinose accumulation related to desiccation tolerance during maize (Zea mays L.) seed development and maturation. Journal of Plant Physiology 150, 481–488.CrossRefGoogle Scholar
Bruggink, T. and van der Toorn, P. (1995) Induction of desiccation tolerance in germinated seeds. Seed Science Research 5, 1–4.CrossRefGoogle Scholar
Bruggink, G.T., Ooms, J.J.J. and van der Toorn, P. (1997) Induction of longevity in primed seeds. Abstracts, p.43 in Symposium on seed biology and technology: Applications and advances, August 13–15, Fort Collins, Colorado.Google Scholar
Bruggink, G.T., Ooms, J.J.J. and van der Toorn, P. (1999) Induction of longevity in primed seeds. Seed Science Research 9, 49–53.CrossRefGoogle Scholar
Buitink, J., Hemminga, M.A. and Hoekstra, F.A. (2000a) Is there a role for oligosaccharides in seed longevity? An assessment of intracellular glass stability. Plant Physiology 122, 1217–1224.CrossRefGoogle Scholar
Buitink, J., Leprince, O., Hemminga, M.A. and Hoekstra, F.A. (2000b) Molecular mobility in the cytoplasm: an approach to describe and predict lifespan of dry germplasm. Proceedings of the National Academy of Sciences USA 97, 2385–2390.CrossRefGoogle ScholarPubMed
Burke, M.J. (1986) The glassy state and survival of anhydrous biological systems. pp. 358–363 in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. Ithaca, NY, Cornell University Press.Google Scholar
Caffrey, M., Fonseca, V. and Leopold, A.C. (1988) Lipid-sugar interactions. Relevance to anhydrous biology. Plant Physiology 86, 754–758.CrossRefGoogle ScholarPubMed
Cronquist, A. (1968) The evolution and classification of flowering plants. London, Nelson.Google Scholar
Crowe, J.H., Crowe, L.M., Carpenter, J.F., Rudolph, A.S., Wistrom, C.A., Spargo, B.J. and Anchordoguy, T.J. (1988) Interactions of sugars with membranes. Biochimica et Biophysica Acta 947, 367–384.CrossRefGoogle ScholarPubMed
Crowe, J.H., Crowe, L.M. and Hoekstra, F.A. (1989) Phase transitions and permeability changes in dry membranes during rehydration. Journal of Bioenergetics and Biomembranes 21, 77–91.CrossRefGoogle ScholarPubMed
Crowe, J.H., Hoekstra, F.A. and Crowe, L.M. (1992) Anhydrobiosis. Annual Review of Physiology 54, 579–599.CrossRefGoogle ScholarPubMed
Dey, P.M. (1990) Oligosaccharides. pp. 189–218 in Dey, P.M.; Harborne, J.B. (Eds) Methods in plant biochemistry. Volume 2. Carbohydrates. New York, Academic Press.Google Scholar
Duke, J.A. (1992) Handbook of phytochemical constituents of GRAS herbs and other economic plants. Boca Raton, CRC Press.Google Scholar
Ellis, R.H. and Roberts, E.H. (1981) The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9, 373–409.Google Scholar
French, D., Youngquist, R.W. and Lee, A. (1959) Isolation and crystallization of planteose from mint seeds. Archives of Biochemistry and Biophysics 85, 471–473.CrossRefGoogle ScholarPubMed
Groot, S.P.C., van der Geest, A.H.M., Tesnier, K., Alonso-Blanco, C., Bentsink, L., Donkers, H., Koornneef, M., Vreugdenhil, D. and Bino, R.J. (2000) Molecular genetic analysis of Arabidopsis seed quality. pp. 123–132 in Black, M.; Bradford, K.J.; Vázquez-Ramos, J. (Eds) Seed biology: Advances and applications. Wallingford, CABI Publishing.Google Scholar
Hoekstra, F.A., Crowe, J.H. and Crowe, L.M. (1992) Germination and ion leakage are linked with phase transitions of membrane lipids during imbibition of Typha latifolia pollen. Physiologia Plantarum 84, 29–34.CrossRefGoogle Scholar
Hoekstra, F.A., Haigh, A.M., Tetteroo, F.A.A. and Van Roekel, T. (1994) Changes in soluble sugars in relation to desiccation tolerance in cauliflower seeds. Seed Science Research 4, 143–147.CrossRefGoogle Scholar
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence on flatulenceproducing oligosaccharides and cyclitols – review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
International Seed Testing Association (1999) International rules for seed testing. Seed Science and Technology (Supplement, Rules) 27, 1–333.Google Scholar
Koster, K.L. and Leopold, A.C. (1988) Sugars and desiccation tolerance in seeds. Plant Physiology 88, 829–832.CrossRefGoogle ScholarPubMed
Leprince, O., Hendry, G.A.F. and McKersie, B.D. (1993) The mechanisms of desiccation tolerance in developing seeds. Seed Science Research 3, 231–246.CrossRefGoogle Scholar
Lin, T.-P., Yen, W.-L. and Chien, C.-T. (1998) Disappearance of desiccation tolerance of imbibed crop seeds is not associated with the decline of oligosaccharides. Journal of Experimental Botany 49, 1203–1212.CrossRefGoogle Scholar
McDonald, M.B. (1999) Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27, 177–237.Google Scholar
Ni, B.R. and Bradford, K.J. (1993) Germination and dormancy of abscisic acid and gibberellin-deficient mutant tomato (Lycopersicon esculentum) seeds. Sensitivity of germination to abscisic acid, gibberellin and water potential. Plant Physiology 101, 607–617.CrossRefGoogle ScholarPubMed
Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Science Research 7, 63–74.CrossRefGoogle Scholar
Obendorf, R.L., Dickerman, A.M., Pflum, T.M., Kacalanos, M.A. and Smith, M.E. (1998) Drying rate alters soluble carbohydrates, desiccation tolerance, and subsequent seedling growth of soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation. Plant Science 132, 1–12.CrossRefGoogle Scholar
Oliver, A.E., Crowe, L.M. and Crowe, J.H. (1998) Methods for dehydration-tolerance: depression of the phase transition temperature in dry membranes and carbohydrate vitrification. Seed Science Research 8, 211–221.CrossRefGoogle Scholar
Ooms, J.J.J., Leon-Kloosterziel, K.M., Bartels, D., Koornneef, M. and Karssen, C.M. (1993) Acquisition of desiccation tolerance and longevity in seeds of Arabidopsis thaliana. A comparative study using abscisic acid-insensitive abi3 mutant. Plant Physiology 102, 1185–1191.CrossRefGoogle Scholar
Sanhewe, A.J. and Ellis, R.H. (1996) Seed development and maturation in Phaseolus vulgaris. II. Post-harvest longevity in air-dry storage. Journal of Experimental Botany 47, 959–965.CrossRefGoogle Scholar
Sun, W.Q. and Leopold, A.C. (1993) The glassy state and accelerated aging of soybeans. Physiologia Plantarum 89, 767–774.CrossRefGoogle Scholar
Tarquis, A.M. and Bradford, K.J. (1992) Prehydration and priming treatments that advance germination also increase the rate of deterioration of lettuce seeds. Journal of Experimental Botany 43, 307–317.CrossRefGoogle Scholar
Townsend, R.R., Hardy, M.R., Hindsgaul, O. and Lee, Y.C. (1988a) High-performance anion-exchange chromatography of oligosaccharides using pellicular resins and pulsed amperometric detection. Analytical Biochemistry 174, 459–470.CrossRefGoogle ScholarPubMed
Townsend, R.R., Hardy, M.R., Olechno, J.D. and Carter, S.R. (1988b) Chromatography of carbohydrates. Nature 335, 379–380.CrossRefGoogle ScholarPubMed
Wattiez, N. and Hans, M. (1943) A holoside extracted from the seeds of Plantago major and Plantago ovata Forsk. Bulletin et Mémoires de l'Academie Royale de Médecine de Belgique (Brussels) 8, 386–396.Google Scholar
Wolkers, W.F., Bochicchio, A., Selvaggi, G. and Hoekstra, F.A. (1998) Fourier transform infrared microspectroscopy detects changes in protein secondary structure associated with desiccation tolerance in developing maize embryos. Plant Physiology 116, 1169–1177.CrossRefGoogle ScholarPubMed
Youcef, A.D., Courtois, J.E. and Le-Dizet, P. (1977) Isolation of trisaccharide planteose from balsamine seeds (Impatiens balsamina). Comptes Rendus Hebdomadaires des Séances de l'Academie des Sciences, Serie D, Sciences Naturelles 285, 1141–1142.Google Scholar