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Influence of temperature on the Pfr level required for germination in lettuce cv. Grand Rapids

Published online by Cambridge University Press:  19 September 2008

David N. Kristie  and
Anthony Fielding
David N. Kristie*
Affiliation:
Department of Biology, Acadia University, Wolfville, Nova Scotia, B0P 1X0, Canada
Anthony Fielding
Affiliation:
Department of Biology, Acadia University, Wolfville, Nova Scotia, B0P 1X0, Canada
*
* Correspondence
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Abstract

The effect of temperature on the Pfr level required to induce germination in lettuce cv. Grand Rapids was investigated. Various Pfr levels were established in seeds, by irradiation with different fluences of red light, or by saturating irradiations with narrow bandwidth light. Both approaches indicated that increasing Pfr levels caused a progressive increase in the upper temperature limit for germination. The Pfr level required to induce 50% germination after a single light pulse increased from about 11% at 15° and 20°C to 86% at 30.5°C. Repeated red light irradiations raised the GT50 (temperature giving 50% germination) by about 2°C, suggesting that Pfr reversion limited germination at high temperatures. However, a large increase in Pfr level required to induce 50% germination was still apparent at high temperatures, even when relatively constant levels of Pfr were maintained by repeated irradiations with narrow bandwidth light. These results indicate that following a single light pulse, germination at high temperatures is limited solely by the availability of Pfr. The precise position of the upper temperature limit for germination is influenced by reversion; however, the underlying cause of thermoinhibition relates to an increasing requirement for Pfr at elevated temperatures.

Keywords

germinationlettucephytochrometemperaturethermoinhibition
Type
Research papers
Information
Seed Science Research , Volume 4 , Issue 1 , March 1994 , pp. 19 - 25
Copyright
Copyright © Cambridge University Press 1994

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Footnotes

1

Present address Department of Botany, University of British Columbia, Vancouver, B.C., V6L 1N9, Canada.

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