Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-26T15:57:34.621Z Has data issue: false hasContentIssue false
  • English
  • Français

Leakage of phosphorus and phytic acid from imbibing seeds and grains

Published online by Cambridge University Press:  19 September 2008

M. Marcia West ,
Irene Ockenden  and
John N. A. Lott
M. Marcia West
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
Irene Ockenden
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
John N. A. Lott*
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Inorganic P, total P and phytic acid-P leakage from imbibing whole soybean, bean, and pea seeds, as well as whole maize and barley grains were measured. The amounts of P and phytic acid leaked during a 6-h soaking period varied for the five species. Soybeans leaked the most P and phytic acid of the species examined. Peas, beans and barley leaked little or no phytic acid. Based on reported levels of total P and phytic acid in the dry seeds/grains that were studied, total P leakage ranged from 0.5 to 1.2%, while phytic acid leakage ranged from 0.01 to 0.5%. No relationship was found between initial levels of total P or phytic acid in the dry seeds/grains and the amount of total P or phytic acid leaked by the imbibed seed/grain. Similarly, the amounts of phytic acid leaked by the seeds/grains of this study did not appear to be related to the water solubility of the phytic acid in the dry seeds/grains.

Keywords

Grainsimbibitioninorganic Pleakagephytic acidseedstotal P
Type
Research Papers
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 97 - 102
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Batten, G.D. (1986) The uptake and utilization of phosphorus and nitrogen by diploid, tetraploid and hexaploid wheats (Triticum spp.). Annals of Botany 58, 49–59.CrossRefGoogle Scholar
Beal, L. and Metha, T. (1985) Zinc and phytate distribution in peas. Influences of heat treatment, germination, pH, substrate and phosphorus on pea phytate and phytase. Journal of Food Science 50, 96–100.CrossRefGoogle Scholar
Beecroft, P. and Lott, J.N.A. (1993) Identification of the sites of K leakage from imbibing seeds and grains. Seed Science Research 3, 105–110.CrossRefGoogle Scholar
Boltz, D.F. and Mellon, M.G. (1948) Spectrophotometric determination of phosphorus as molybdiphosphoric acid. Analytical Chemistry 20, 749–751.CrossRefGoogle Scholar
Bruggink, H., Kraak, H.L., Dijkema, M.H.G.E. and Bekendam, J. (1991) Some factors influencing electrolyte leakage from maize (Zea mays L.) kernels. Seed Science Research 1, 15–20.CrossRefGoogle Scholar
Cavdek, V., Lott, J.N.A. and Kerr, P. (1987) Cell wall wrinkling and solute leakage in imbibing squash and carrot seeds. Scanning Microscopy 1, 1413–1421.Google Scholar
Clutterbuck, V.J. and Briggs, D.E. (1974) Phosphate mobilization in grains of Hordeum distichon. Phytochemistry 13, 45–54.CrossRefGoogle Scholar
Dawidowicz-Grzegorzewska, A. and Podstolski, A. (1992) Age-related changes in the ultrastructure and membrane properties of Brassica napus L. seeds. Annals of Botany 69, 39–46.CrossRefGoogle Scholar
de Boland, A.R., Garner, G.B. and O'Dell, B.L. (1975) Identification and properties of phytate in cereal grains and oilseed products. Journal of Agricultural and Food Chemistry 23, 1186–1189.CrossRefGoogle ScholarPubMed
Deosthale, Y.G. (1979) Mineral and trace element composition of maize. Current Science 48, 54–55.Google Scholar
Duke, S.H. and Kakefuda, G. (1981) Role of the testa in preventing cellular rupture during imbibition of legume seeds. Plant Physiology 67, 449–456.CrossRefGoogle ScholarPubMed
Givelberg, A., Horowitz, M. and Poljakoff-Mayber, A. (1984) Solute leakage from Solanum nigrum L. seeds exposed to high temperatures during imbibition. Journal of Experimental Botany 35, 1754–1763.CrossRefGoogle Scholar
Greenwood, J.S. (1989) Phytin synthesis and deposition. pp 109–125 in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Harland, B.F. and Oberleas, D. (1986) Anion-exchange method for determination of phytate in foods: collaborative study. Journal of the Association of Official Analytical Chemists 69, 667–670.Google ScholarPubMed
Hernández-Unzón, H. and Ortega-Delgado, M.L. (1989) Phytic acid in stored common bean seeds (Phaseolus vulgaris L.). Plant Foods for Human Nutrition 39, 209–221.CrossRefGoogle ScholarPubMed
Isaac, R.A. (1990) Phosphorus in plants: micro-method. in Helrich, K. (Ed.) Official methods of analysis of the Association of Official Analytical Chemists. Vol. 1. 15th ed. Chap. 3. Plants. Arlington, VA., Association of Official Analytical Chemists (AOAC), Inc.Google Scholar
Ishida, N., Kano, H., Kobayashi, T., Hamaguchi, H. and Yoshida, T. (1988) The relationship between imbibitional damage and initial water content of soybeans. Agricultural and Biological Chemistry 52, 2771–2775.Google Scholar
Larson, L.A. (1968) The effect soaking pea seeds with or without seedcoats has on seedling growth. Plant Physiology 43, 255–259.CrossRefGoogle ScholarPubMed
Lolas, G.M. and Markakis, P. (1975) Phytic acid and other phosphorus compounds of beans (Phaseolus vulgaris L.). Journal of Agricultural and Food Chemistry 23, 13–15.CrossRefGoogle Scholar
Lolas, G.M., Palamidis, N. and Markakis, P. (1976) The phytic acid-total phosphorus relationship in barley, oats, soybeans, and wheat. Cereal Chemistry 53, 867–871.Google Scholar
Loomis, E.L. and Smith, O.E. (1980) The effect of artificial aging on the concentration of Ca, Mg, Mn, K, and Cl in imbibing cabbage seed. Journal of the American Society for Horticultural Science 105, 647–650.CrossRefGoogle Scholar
Lott, J.N.A. (1984) Accumulation of seed reserves of phosphorus and other minerals. pp. 139–166 in Murray, D.R. (Ed.) Seed Physiology. Vol. 1. Development. Sydney, Academic Press.Google Scholar
Lott, J.N.A., Randall, P.J., Goodchild, D.J. and Craig, S. (1985) Occurrence of globoid crystals in cotyledonary protein bodies of Pisum sativum as influenced by experimentally induced changes in Mg, Ca and K contents of seeds. Australian Journal of Plant Physiology 12, 341–353.Google Scholar
Lott, J.N.A., Cavdek, V. and Carson, J. (1991) Leakage of K, Mg, Cl, Ca and Mn from imbibing seeds, grains and isolated seed parts. Seed Science Research 1, 229–233.CrossRefGoogle Scholar
Maga, J.A. (1982) Phytate: its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis. Journal of Agricultural and Food Chemistry 30, 1–9.CrossRefGoogle Scholar
McDonald, M.B. Jr., Vertucci, C.W. and Roos, E.E. (1988) Seed coat regulation of soybean seed imbibition. Crop Science 28, 987–992.CrossRefGoogle Scholar
McKersie, B.D. and Stinson, R.H. (1980) Effect of dehydration on leakage and membrane structure in Lotus corniculatus L. seeds. Plant Physiology 66, 316–320.CrossRefGoogle ScholarPubMed
Mengel, K. and Kirkby, E.A. (1982) Principles of plant nutrition. 3rd ed. Bern, Switzerland, International Potash Institute.Google Scholar
O'Dell, B.L., de Boland, A.R. and Koirtyohann, S.R. (1972) Distribution of phytate and nutritionally important elements among the morphological components of cereal grains. Journal of Agricultural and Food Chemistry 20, 718–721.CrossRefGoogle Scholar
Parrish, F.W., Madacsi, J.P., Phillippy, B.Q., Wilfred, A.G. and Buco, S.M. (1990) Determination of phytic acid in cottonseed by near-infrared reflectance spectroscopy. Journal of Agricultural and Food Chemistry 38, 407–409.CrossRefGoogle Scholar
Paul, A.A. and Southgate, D.A.T. (1978) The composition of foods. 4th ed. Amsterdam, New York, Oxford, Elsevier/North-Holland Biomedical Press.Google Scholar
Plaami, S. and Kumpulainen, J. (1991) Determination of phytic acid in cereals using ICP-AES to determine phosphorus. Journal of the Association of Official Analytical Chemists 74, 32–36.Google ScholarPubMed
Prattley, C.A. and Stanley, D.W. (1982) Protein-phytate interactions in soybeans. I. Localization of phytate in protein bodies and globoids. Journal of Food Biochemistry 6, 243–253.CrossRefGoogle Scholar
Raboy, V. (1990) Biochemistry and genetics of phytic acid synthesis. pp 55–76 in Morré, D.J., Boss, W.F. and Loewus, F.A. (Eds.) Inositol metabolism in plants. Vol. 9. New York, Wiley-Liss, Inc.Google Scholar
Raboy, V. and Dickinson, D.B. (1984) Effect of phosphorus and zinc nutrition on soybean seed phytic acid and zinc. Plant Physiology 75, 1094–1098.CrossRefGoogle ScholarPubMed
Reichert, R.D. and MacKenzie, S.L. (1982) Composition of peas (Pisum sativum) varying widely in protein content. Journal of Agricultural and Food Chemistry 30, 312–317.CrossRefGoogle Scholar
Roberts, E.H. and Roberts, D.L. (1972) Moisture content of seeds. pp 424–429 in Roberts, E.H. (Ed.) Viability of seeds. London, Chapman and Hall Ltd.CrossRefGoogle Scholar
Rowland, G.G. and Gusta, L.V. (1977) Effects of soaking, seed moisture content, temperature and seed leakage on germination of faba beans (Vicia faba) and peas (Pisum sativum). Canadian Journal of Plant Science 57, 401–406.CrossRefGoogle Scholar
Sandstead, H.H. (1992) Fiber, phytates, and mineral nutrition. Nutrition Reviews 50, 30–31.CrossRefGoogle ScholarPubMed
Schoettle, A.W. and Leopold, A.C. (1984) Solute leakage from artificially aged soybean seeds after imbibition. Crop Science 24, 835–838.CrossRefGoogle Scholar
Stewart, A., Nield, H. and Lott, J.N.A. (1988) An investigation of the mineral content of barley grains and seedlings. Plant Physiology 86, 93–97.CrossRefGoogle ScholarPubMed
Takemasa, M. and Hijikuro, S. (1984) Relationship between total phosphorus and phytate phosphorus in plant-source ingredients, especially in domestic barley. Bulletin of National Institute of Animal Industry 42, 37–39.Google Scholar
Thornton, J.M., Powell, A.A. and Matthews, S. (1990) Investigation of the relationship between seed leachate conductivity and the germination of Brassica seed. Annals of Applied Biology 117, 129–135.CrossRefGoogle Scholar
Welch, R.M., House, W.A. and Allaway, W.H. (1974) Availability of zinc from pea seeds to rats. Journal of Nutrition 104, 733–740.CrossRefGoogle ScholarPubMed
West-Knights, J. (1880) On the estimation of phosphoric acid in potable water. Analyst 5, 195–196.CrossRefGoogle Scholar