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A meta-analysis of the effects of treatments used to break dormancy in seeds of the megagenus Astragalus (Fabaceae)

Published online by Cambridge University Press:  04 November 2020

Elias Soltani Open the ORCID record for Elias Soltani [Opens in a new window] ,
Jerry M. Baskin ,
Carol C. Baskin  and
Fatemeh Benakashani
Elias Soltani*
Affiliation:
Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Iran
Jerry M. Baskin
Affiliation:
Department of Biology, University of Kentucky, Lexington, KY40506-0225, USA
Carol C. Baskin
Affiliation:
Department of Biology, University of Kentucky, Lexington, KY40506-0225, USA Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY40546-0312, USA
Fatemeh Benakashani
Affiliation:
Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Iran
*
Author for Correspondence: Elias Soltani, E-mail: elias.soltani@ut.ac.ir
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Abstract

Astragalus is the largest genus of seed plants with 3000 or more species that occurs naturally on several continents. The genus has some use as a forage and medicine and in industry, and many of the species are rare endemics threatened with extinction. The seeds are reported to be dormant at maturity, and various treatments have been used in an attempt to germinate them. Our primary aim was to determine via a meta-analysis the most effective way(s) to break dormancy in seeds of this species-rich genus. Mechanical and chemical (conc. sulphuric acid) scarification were by far the best of 12 treatments for breaking seed dormancy of the 40 species included in our meta-analysis, whereas prechilling, gibberellin and smoke were ineffective. These results along with those of imbibition tests confirm that seeds of the examined Astragalus species have physical dormancy (PY). Further, PY in these 40 species and (its documented occurrence) in 118 species that could not be included in our meta-analysis transcends climatic and geographic boundaries, edaphic conditions, life cycle/life form types and infrageneric phylogeny. Thus, it seems likely that most species of Astragalus have PY. However, in addition to PY, physiological dormancy (PD), that is, combinational dormancy (PY + PD), has been reported in a few species of Astragalus. This study should be useful to both basic and applied scientists who want to germinate seeds of Astragalus.

Keywords

chemical scarificationcombinational dormancydormancy-breaking treatmentsimpermeable seed coatmechanical scarificationphysical dormancy
Type
Research Paper
Information
Seed Science Research , Volume 30 , Issue 3 , September 2020 , pp. 224 - 233
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Abudureheman, B, Liu, H, Zhang, D and Guan, K (2014) Identification of physical dormancy and dormancy release patterns in several species (Fabaceae) of the cold desert, north-west China. Seed Science Research 24, 133–145.CrossRefGoogle Scholar
Acharya, SN, Kokko, EG and Fraser, J (1993) Storage duration and freeze-thaw effects on germination and emergence of cicer milkvetch (Astragalus cicer) seeds. Journal of Seed Technology 17, 9–21.Google Scholar
Acharya, SN, Kastelic, JP, Beauchemin, KA and Messenger, DF (2006) A review of research progress on cicer milkvetch (Astragalus cicer L.). Canadian Journal of Plant Science 86, 49–62.10.4141/P04-174CrossRefGoogle Scholar
Agh, K, Esmaeili, MM, Hossinimoghaddam, H and Mostafalo, H (2017) The effect of different treatments on seed dormancy and germination of Astragalus podolobus. Nova Biologica Reperta 4, 147–154 (in Persian).Google Scholar
Albrecht, MA and Long, QG (2019) Habitat suitability and herbivores determine reintroduction success of an endangered legume. Plant Diversity 41, 109–117.10.1016/j.pld.2018.09.004CrossRefGoogle ScholarPubMed
Albrecht, MA and McCue, KA (2010) Changes in demographic processes over long time scales reveal the challenge of restoring an endangered plant. Restoration Ecology 18, 235–243.10.1111/j.1526-100X.2009.00584.xCrossRefGoogle Scholar
Albrecht, MA and Penagos, JC (2012) Seed germination ecology of three imperiled plants of rock outcrops in the southeastern United States. Journal of the Torrey Botanical Society 139, 86–96.10.3159/TORREY-D-11-00066.1CrossRefGoogle Scholar
Arbabian, S, Moghanloo, M and Majd, A (2009) Seed dormancy breakage methods in the endangered species Astragalus fridae Rech. Journal of Animal Physiology and Development 2, 45–50 (in Persian).Google Scholar
Arène, F, Affre, L, Doxa, A and Saatkamp, A (2017) Temperature but not moisture response of germination shows phylogenetic constraints while both interact with seed mass and life form. Seed Science Research 27, 110–120.10.1017/S0960258517000083CrossRefGoogle Scholar
Bacchetta, G, Fenu, G, Mattana, E and Pontecorvo, C (2011) Ecological remarks on Astragalus maritimus and A. verrucosus, two threatened exclusive endemic species of Sardinia. Acta Botanica Gallica 158, 79–91.10.1080/12538078.2011.10516256CrossRefGoogle Scholar
Baer, KC and Maron, JL (2018) Pre-dispersal seed predation and pollen limitation constrain population growth across the geographic distribution of Astragalus utahensis. Ecology 106, 1646–1659.10.1111/1365-2745.12932CrossRefGoogle Scholar
Barneby, RC (1964) Atlas of North American Astragalus. Memoirs of the New York Botanical Garden 13, 1–1188.Google Scholar
Baskin, JM and Baskin, CC (1981) A contribution to the ecological life cycle of Astragalus tennesseensis. Journal of the Tennessee Academy of Science 56, 4–6.Google Scholar
Baskin, CC and Baskin, JM (2014) Seeds: ecology, biogeography, and evolution of dormancy and germination (2nd edn). San Diego, Elsevier/Academic Press.Google Scholar
Baskin, CC and Quarterman, E (1969) Germination requirements of seeds of Astragalus tennesseensis. Bulletin of the Torrey Botanical Club 96, 315–321.10.2307/2483736CrossRefGoogle Scholar
Becker, T (2010) Explaining rarity of the dry grassland perennial Astragalus exscapus. Folia Geobotanica 45, 303–321.10.1007/s12224-010-9068-3CrossRefGoogle Scholar
Becker, T, Voss, N and Durka, W (2011) Pollen limitation and inbreeding depression in an ‘old rare’ bumblebee pollinated grassland herb. Plant Biology 13, 857–864.CrossRefGoogle Scholar
Bowles, ML (1988) Memo: Astragalus tennesseensis restoration at Wea Gravel Prairie, Tippecanoe County, Indiana. Unpublished Report to Indian Division of Nature Preserves for the Report to the U.S. Fish and Wildlife Service Endangered Species Program.Google Scholar
Bowles, ML, Bachtell, KR, DeMauro, MM, Sykora, LG and Bautista, CR (1988) Propagation techniques used in establishing a greenhouse population of Astragalus tennesseensis Gray. Natural Areas Journal 8, 121–122.Google Scholar
Bowles, ML, Betz, RF and DeMauro, MM (1993) Propagation of rare plants from historic seed collections: implications for species restoration and herbarium management. Restoration Ecology 1, 101–106.CrossRefGoogle Scholar
Bushman, BS, Johnson, DA, Connors, KJ and Jones, TA (2015) Germination and seedling emergence of three semiarid western North American legumes. Rangeland Ecology & Management 68, 501–506.10.1016/j.rama.2015.08.002CrossRefGoogle Scholar
Bushman, BS, Horning, ME, Shock, CC, Feibert, EB and Johnson, DA (2019) Dryland seedling emergence of basalt milkvetch (Astragalus filipes) and western prairie clover (Dalea ornata) under different planting seasons and seed treatments. Native Plants Journal 20, 239–243.10.3368/npj.20.3.239CrossRefGoogle Scholar
Butkutė, B, Padarauskas, A, Cesevičienė, J, Taujenis, L and Norkevičienė, E (2018) Phytochemical composition of temperate perennial legumes. Crop and Pasture Science 69, 1020–1030.10.1071/CP18206CrossRefGoogle Scholar
Cavieres, LA and Sierra-Almeida, A (2018) Assessing the importance of cold-stratification for seed germination in alpine plant species of the High-Andes of central Chile. Perspectives in Plant Ecology, Evolution and Systematics 30, 125–131.10.1016/j.ppees.2017.09.005CrossRefGoogle Scholar
Chaudhary, LB, Rana, TS and Anand, KK (2008) Current status of the systematics of Astragalus L. (Fabaceae) with special reference to the Himalayan species in India. Taiwania 53, 338–355.Google Scholar
Cho, HJ and Widholm, JM (2002) Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus. Plant Cell, Tissue and Organ Culture 69, 259–269.10.1023/A:1015624316573CrossRefGoogle Scholar
Cook, D, Ralphs, MH, Welch, KD and Stegelmeier, BL (2009) Locoweed poisoning in livestock. Rangelands 31, 16–21.10.2111/1551-501X-31.1.16CrossRefGoogle Scholar
Cook, D, Gardner, DR, Martinez, A, Robles, CA and Pfister, JA (2017) Screening for swainsonine among South American Astragalus species. Toxicon 139, 54–57.10.1016/j.toxicon.2017.09.014CrossRefGoogle ScholarPubMed
Cox, RD, Chou, YF and Wester, DB (2017) Smoke water and heat influence emergence of shortgrass prairie species. Fire Ecology 13, 138–148.10.4996/fireecology.1301138CrossRefGoogle Scholar
Decker, K (2005) Astragalus wetherillii Jones (Wetherill's milkvetch): a technical conservation assessment. Lakewood, CO, USDA Forest Service, Rocky Mountain Region.Google Scholar
Decker, K (2006) Astragalus missouriensis Nutt. var. humistratus Isely (Missouri milkvetch): a technical conservation assessment. Lakewood, CO, USDA Forest Service, Rocky Mountain Region.Google Scholar
Dianati Tilaki, GA, Naghipour Borj, AA, Tavakoli, H and Haidarian Aghakhani, M (2010) The effects of exclosure on plants in the semi-arid rangeland of North Khorasan province, Iran. Desert 15, 45–52.Google Scholar
Dilaver, Z, Mirzapour, M and Kendir, H (2017) Breaking seed dormancy and micropropagation of perennial vulneraria milkvetch (Astragalus vulnerariae DC.). Acta Scientiarum Polonorum, Hortorum Cultus 16, 79–88.CrossRefGoogle Scholar
Dziurka, K, Skrzypek, E and Dubert, F (2019) Breaking seed dormancy of Astragalus penduliflorus Lam. Acta Societatis Botanicorum Poloniae 88, 3617.CrossRefGoogle Scholar
Eisvand, H, Madah, AH and Tavakol, AR (2005) Study of dormancy breakage and germination in seeds of Astragalus siliquosus. Rangelands and Forests Plant Breeding and Genetic Research 13, 67–84 (in Persian).Google Scholar
Eisvand, HR, Arefi, HM and Tavakol-Afshari, R (2006) Effects of various treatments on breaking seed dormancy of Astragalus siliquosus. Seed Science and Technology 34, 747–752.CrossRefGoogle Scholar
Erisen, S, Yorgancilar, M, Atalay, E, Babaoglu, M and Duran, A (2010) Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey. Electronic Journal of Biotechnology 13, 13–14.CrossRefGoogle Scholar
Fateh, E, Majnoun, HN, Madah, AH and Sharifzadeh, F (2006) Seed dormancy methods [of] breakage in Astragalus tribuloides. Rangelands and Forests Plant Breeding and Genetic Research 13, 345–360 (in Persian).Google Scholar
Ferhi, F, Das, S, Elaloui, E, Moussaoui, Y and Yanez, JG (2014) Chemical characterisation and suitability for papermaking applications studied on four species naturally growing in Tunisia. Industrial Crops and Products 61, 180–185.CrossRefGoogle Scholar
Fotheringham, CJ and Keeley, JE (1998) Ecology and distribution of Braunton's milkvetch (Astragalus brauntonii) and Lyon's pentachaeta (Pentachaeta lyonii). Long Beach, CA, USA, California Department of Fish and Game.Google Scholar
Frodin, DG (2004) History and concepts of big plant genera. Taxon 53, 753–776.CrossRefGoogle Scholar
Fund, AJ, Hulvey, KB, Jensen, SL, Johnson, DA, Madsen, MD, Monaco, TA, Tilley, DJ, Arora, E and Teller, B (2019) Basalt milkvetch responses to novel restoration treatments in the Great Basin. Rangeland Ecology and Management 72, 492–500.CrossRefGoogle Scholar
Gurevitch, J and Hedges, LV (1999) Statistical issues in ecological meta-analyses. Ecology 80, 1142–1149.CrossRefGoogle Scholar
Han, YJ, Baskin, JM, Tan, DY, Baskin, CC and Wu, MY (2018) Effects of predispersal insect seed predation on the early life history stages of a rare cold sand-desert legume. Scientific Reports 8, 3240.CrossRefGoogle ScholarPubMed
Hedges, LV, Gurevitch, J and Curtis, PS (1999) The meta-analysis of response ratios in experimental ecology. Ecology 80, 1150–1156.CrossRefGoogle Scholar
Hou, SW and Jia, JF (2004) High frequency plant regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis. Plant Cell, Tissue and Organ Culture 79, 95–100.CrossRefGoogle Scholar
Hung, CY and Xie, J (2008) Development of an efficient plant regeneration system for the selenium-hyperaccumulator Astragalus racemosus and the nonaccumulator Astragalus canadensis. HortScience 43, 2138–2142.CrossRefGoogle Scholar
Ikram, RM, Tanveer, A, Ata, Z and Saqib, M (2014) Dormancy studies on Euphorbia dracunculoides and Astragalus spp.: major weeds of arid areas. Planta Daninha 32, 747–753.CrossRefGoogle Scholar
International Union for the Conservation of Nature (IUCN) (2019) The IUCN red list of threatened species. Available at: http://www.iucnredlist.org/ (accessed 1 October 2019).Google Scholar
Jones, TA, Johnson, DA, Bushman, BS, Connors, KJ and Smith, RC (2016) Seed dormancy mechanisms in basalt milkvetch and western prairie clover. Rangeland Ecology & Management 69, 117–122.CrossRefGoogle Scholar
Kaye, TN (1997) Seed dormancy in high elevation plants: implications for ecology and restoration, pp. 115–120 in Kaye, TN; Liston, A; Love, RM; Luoma, DL; Meinke, RJ and Wilson, MV (Eds) Conservation and management of native plants and fungi. Corvallis, OR, Native Plant Society of Oregon.Google Scholar
Kaye, TN (1999) From flowering to dispersal: reproductive ecology of an endemic plant, Astragalus australis var. olympicus (Fabaceae). American Journal of Botany 86, 1248–1256.CrossRefGoogle Scholar
Keshtkar, AR, Keshtkar, HR, Razavi, SM and Dalfardi, S (2008) Methods to break seed dormancy of Astragalus cyclophyllon. African Journal of Biotechnology 7, 3874–3877.Google Scholar
Khayat Moghadam, M and Sadrabadi Haghighi, R (2015) Evaluation of seed dormancy breaking methods in Astragalus parrowianus. International Journal of Farming and Allied Sciences 4, 473–476.Google Scholar
Kheloufi, A, Mansouri, LM, Bouafia, B, Khamari, Y, Kheloufi, H and Bouguern, Y (2018) Morphological characteristics and seed germination improvement of two ecotypes of Astragalus armatus Willd. subsp. armatus in Algeria. Cercetări Agronomice în Moldova 1, 96–107.CrossRefGoogle Scholar
Kildisheva, OA, Erickson, TE, Merritt, DJ, Madsen, MD, Dixon, KW, Vargas, J, Amarteifio, R and Kramer, AT (2018) Do abrasion- or temperature-based techniques more effectively relieve physical dormancy in seeds of cold desert perennials? Rangeland Ecology & Management 71, 318–322.CrossRefGoogle Scholar
Kildisheva, OA, Erickson, TE, Madsen, MD, Dixon, KW and Merritt, DJ (2019) Seed germination and dormancy traits of forbs and shrubs important for restoration of North American dryland ecosystems. Plant Biology 21, 458–469.CrossRefGoogle ScholarPubMed
Kim, SY, Oh, SH, Hwang, WH, Kim, SM, Choi, KJ and Kang, HW (2008) Physical dormancy in seeds of Chinese milk vetch (Astragalus sinicus L.) from Korea. Korean Journal of Crop Science 53, 421–426.Google Scholar
Kimura, E and Islam, MA (2012) Seed scarification methods and their use in forage legumes. Research Journal of Seed Science 5, 38–50.Google Scholar
Kondo, T and Takeuchi, S (2004) Breaking seed dormancy and growth after germination of Astragalus adsurgens (Leguminosae), a rare species in Hokkaido. Journal of the Japanese Society of Revegetation 29, 495–502.CrossRefGoogle Scholar
Kunz, M, Randall, JL, Gray, JB, Wall, WA and Hohmann, MG (2016) Germination and propagation of Astragalus michauxii, a rare southeastern US endemic legume. Native Plants Journal 17, 47–52.CrossRefGoogle Scholar
Langran, X (2010) 139. Astragalus Linnaeus. Flora of China, Species Plantarum 2: 755.Google Scholar
Lee, YH, Hong, SH, Kang, BH, Lee, JK, Shim, SI and Kim, W (2006) Effect of stratification on the dormancy of Chinese milk vetch seeds (Astragalus sinicus L.). Korean Journal of Plant Breeding 38, 250–254.Google Scholar
Lesica, P (1995) Demography of Astragalus scaphoides and effects of herbivory on population growth. The Great Basin Naturalist 55, 142–150.Google Scholar
Li, S, Li, SK, Gan, RY, Song, FL, Kuang, L and Li, HB (2013) Antioxidant capacities and total phenolic contents of infusions from 223 medicinal plants. Industrial Crops and Products 51, 289–298.CrossRefGoogle Scholar
Li, X, Qu, L, Dong, Y, Han, L, Liu, E, Fang, S, Zhang, Y and Wang, T (2014) A review of recent research progress on the Astragalus genus. Molecules 19, 18850–18880.CrossRefGoogle ScholarPubMed
Liu, P, Zhao, H and Luo, Y (2017) Anti-aging implications of Astragalus membranaceus (Huangqi): a well-known Chinese tonic. Aging and Disease 8, 868–886.CrossRefGoogle ScholarPubMed
Long, Y, Tan, DY, Baskin, CC and Baskin, JM (2012) Seed dormancy and germination characteristics of Astragalus arpilobus (Fabaceae, subfamily Papilionoideae), a central Asian desert annual ephemeral. South African Journal of Botany 83, 68–77.CrossRefGoogle Scholar
Lôpez-Franco, Y and Higuera-Ciapara, I (2009) Other exudates: tragacanth, karaya, mesquite gum and larchwood arabinogalactans, pp. 495–525 in Phillips, GO; Williams, PA (Eds) Handbook of hydrocolloids. Boca Raton, CRC Press.CrossRefGoogle Scholar
Luo, JP and Jia, JF (1998) Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens. Plant Cell Reports 17, 567–570.CrossRefGoogle ScholarPubMed
Mabberley, DJ (2008) Mabberley's plant-book. A portable dictionary of plants, their classification and uses (3rd edn). Cambridge, Cambridge University Press.Google Scholar
Martínez-Fernández, V, Martínez-García, F and Pérez-García, F (2014) Census, reproductive biology, and germination of Astragalus gines-lopezii (Fabaceae), a narrow and endangered endemic species of SW Spain. Turkish Journal of Botany 38, 686–695.CrossRefGoogle Scholar
Masumi, AA (2018) Flora of Iran. Fabaceae family, Astragalus III genus. Karaj, Iran, Research Institute of Forest Research Publishing (in Persian).Google Scholar
Maunder, M (1992) Plant reintroduction: an overview. Biodiversity & Conservation 1, 51–61.CrossRefGoogle Scholar
Meinke, R, Meyers, S, Amsberry, K, Wilson, C, Groberg, M, Woolverton, R and Brown, J (2013) Assessing the population genetics, taxonomy, reproductive ecology, and life history traits of Humboldt milk-vetch (Astragalus agnicidus) in relation to conservation and management. Eureka, CA, California Department of Fish and Wildlife, Eureka Field Office.Google Scholar
Miklas, PN, Townsend, CE and Ladd, SL (1987) Seed coat anatomy and the scarification of cicer milkvetch seed. Crop Science 27, 766–772.CrossRefGoogle Scholar
Moammeri, M, Abasi Khalaki, M and Tavili, A (2012) Investigation effect of different seed dormancy breaking treatments on germination of, Astragalus gossypinus Fisch. and Zygophyllum eurypterum Boiss. & Buhse seeds. Watershed Management Research (Pajouhesh and Sazandegi) 101, 12–22 (in Persian).Google Scholar
Molnár, VA, Sonkoly, J, Lovas-Kiss, Á, Fekete, R, Takacs, A, Somlyay, L and Toeroek, P (2015) Seed of the threatened annual legume, Astragalus contortuplicatus, can survive over 130 years of dry storage. Preslia 87, 319–328.Google Scholar
Moshtaghyan, MB, Keshtkar, HR, Sharif, ME and Razavi, SM (2009) Planting methods effect on Astragalus cyclophyllon establishment. Iranian Journal of Range and Desert Research 16, 79–84 (in Persian).Google Scholar
Naseri, S and Adibi, MA (2016) Reintroduction of endangered species Astragalus fridae Rech. F. to the nature. Second International Conference of IALE-Iran, Isfahan, Iran, University of Technology.Google Scholar
NatureServe (2019) NatureServe Explorer: an online encyclopedia of life. Available at: http://explorer.natureserve.org (accessed 1 October 2019).Google Scholar
Neyeloff, JL, Fuchs, SC and Moreira, LB (2012) Meta-analyses and forest plots using a Microsoft Excel spreadsheet: step-by-step guide focusing on descriptive data analysis. BMC Research Notes 5, 52.CrossRefGoogle ScholarPubMed
Patanè, C and Gresta, F (2006) Germination of Astragalus hamosus and Medicago orbicularis as affected by seed-coat dormancy breaking techniques. Journal of Arid Environments 67, 165–173.CrossRefGoogle Scholar
Pearson, T (2015) Astragalus peckii population establishment: developing protocols for seed germination, seedling cultivation, and planting. Honors Scholar thesis, Oregon State University, Corvallis.Google Scholar
Pickart, AJ, Hiss, AE and Enberg, AW (1992) Return from extinction: recovery of the Humboldt milk-vetch, pp. 255–261 in Proceedings of the symposium on biodiversity of northwest California, October 28–30, Santa Rosa, California.Google Scholar
Ramos, S, Rincón, S and Vázquez, FM (2010) Distribution and germination characteristics of Astragalus gines-lopezii: an endangered species. Annales Botanici Fennici 47, 330–338.Google Scholar
Rittenhouse, B and Rosentreter, R (1994) The autecology of Challis milkvetch, an endemic of east-central Idaho. Natural Areas Journal 14, 22–30.Google Scholar
Robertson, AW, Trass, A, Ladley, JJ and Kelly, D (2006) Assessing the benefits of frugivory for seed germination: the importance of the deinhibition effect. Functional Ecology 20, 58–66.CrossRefGoogle Scholar
Rosbakh, S, Baskin, CC and Baskin, JM (2020) Nikolaeva et al.'s reference book on seed dormancy and germination. Ecology 101, e03049.CrossRefGoogle ScholarPubMed
Rosenberg, MS, Garrett, KA, Su, Z and Bowden, RL (2004) Meta-analysis in plant pathology: synthesizing research results. Phytopathology 94, 1013–1017.CrossRefGoogle ScholarPubMed
Rubio de Casas, R, Willis, CG, Pearse, WD, Baskin, CC, Baskin, JM and Cavender-Bares, J (2017) Global biogeography of seed dormancy is determined by seasonality and seed size: a case study in the legumes. New Phytologist 214, 1527–1535.CrossRefGoogle ScholarPubMed
Sahbaz, R, Lieberei, R and Aniszewski, T (2012) Polyphenol oxidase (PPO, catecholase) activity during germination and early seedling growth of Cicer milkvetch (Astragalus cicer L.). Journal of Applied Botany and Food Quality 82, 163–169.Google Scholar
Schnadelbach, A, Veiga-Barbosa, L, Ruiz, C, Pita, JM and Pérez-García, F (2016) Dormancy breaking and germination of Adenocarpus desertorum, Astragalus gines-lopezii and Hippocrepis grosii (Fabaceae) seeds, three threatened endemic Spanish species. Seed Science and Technology 44, 1–14.CrossRefGoogle Scholar
Schurr, L, Affre, L, Flacher, F, Tatoni, T, Pecheux, LLM and Geslin, B (2019) Pollination insights for the conservation of a rare threatened plant species, Astragalus tragacantha (Fabaceae). Biodiversity and Conservation 28, 1389–1409.CrossRefGoogle Scholar
Seglias, AE, Williams, E, Bilge, A and Kramer, AT (2018) Phylogeny and source climate impact seed dormancy and germination of restoration relevant forb species. PLoS ONE 13, e0191931.CrossRefGoogle ScholarPubMed
Segura, F, Vicente, MJ, Franco, JA and Martínez-Sánchez, JJ (2015) Effects of maternal environmental factors on physical dormancy of Astragalus nitidiflorus seeds (Fabaceae), a critically endangered species of SE Spain. Flora 216, 71–76.CrossRefGoogle Scholar
Shibata, T and Hatakeyama, Y (1995) Breaking of dormancy in the seeds of Astragalus mongholicus Bunge (Leguminosae). Journal of Plant Physiology 146, 366–368.CrossRefGoogle Scholar
Siles, G, Torres, JA, Ruiz-Valenzuela, L and García-Fuentes, A (2016) Germination trials of annual autochthonous leguminous species of interest for planting as herbaceous cover in olive groves. Agriculture Ecosystems & Environment 217, 119–127.Google Scholar
Siles, G, Zafra, ÁG, Torres, JA, Fuentes, AG and Valenzuela, LR (2017) Germination success under different treatments and pod sowing depths in six legume species present in olive groves. Spanish Journal of Agricultural Research 15, e1007.CrossRefGoogle Scholar
Smreciu, EA, Currah, RS and Toop, E (1988) Viability and germination of herbaceous perennial species native to southern Alberta grasslands. Canadian Field-Naturalist 102, 31–38.Google Scholar
Soltani, E and Soltani, A (2015) Meta-analysis of seed priming effects on seed germination, seedling emergence and crop yield: Iranian studies. International Journal of Plant Production 9, 413–432.Google Scholar
Soltani, E, Baskin, CC, Baskin, JM, Heshmati, S and Mirfazeli, MS (2018) A meta-analysis of the effects of frugivory (endozoochory) on seed germination: role of seed size and kind of dormancy. Plant Ecology 219, 1283–1294.CrossRefGoogle Scholar
Spellenberg, R (1976) Chromosome numbers and their cytotaxonomic significance for North American Astragalus (Fabaceae). Taxon 25, 463–476.CrossRefGoogle Scholar
Statwick, JM (2016) Germination pretreatments to break hard-seed dormancy in Astragalus cicer L.(Fabaceae). PeerJ 4, e2621.CrossRefGoogle Scholar
Tahmasebi, P, Manafian, N, Ebrahimi, A, Omidipour, R and Faal, M (2020) Managing grazing intensity linked to forage quantity and quality trade-off in semiarid rangelands. Rangeland Ecology and Management 73, 53–60.CrossRefGoogle Scholar
Tavili, A, Abasi, M and Moameri, M (2012) The effects of different dormancy breaking treatments on germination and seedling growth of Astragalus gossypinus. Iranian Journal of Seed Science and Technology 1, 64–72 (in Persian).Google Scholar
Tavili, A, Mirdashtvan, M, Alijani, R, Yousefi, M and Zare, S (2014) Effect of different treatments on improving seed germination characteristics of Astragalus adscendens and Astragalus podolobus. Journal of Rangeland Science 4, 110–117.Google Scholar
Townsend, CE and McGinnies, WJ (1972) Mechanical scarification of cicer milkvetch (Astragalus cicer L.) seed. Crop Science 12, 392–394.CrossRefGoogle Scholar
Traveset, A and Verdu, M (2002) A meta-analysis of the effect of gut treatment on seed germination, pp. 339–350 in Levey, DJ; Silva, WR and Galetti, M (Eds) Seed dispersal and frugivory: ecology, evolution and conservation. Wallingford, CABI International.Google Scholar
Turgut-Kara, N and Ari, S (2008) In vitro plant regeneration from embryogenic cell suspension culture of Astragalus chrysochlorus (Leguminoseae). African Journal of Biotechnology 7, 1250–1255.Google Scholar
Uranbey, S, Çöçü, S, Sancak, C, Parmaksız, İ, Khawar, KM, Mirici, S and Özcan, S (2003) Efficient adventitious shoot regeneration in cicer milkvetch. Biotechnology and Biotechnological Equipment 17, 33–37.CrossRefGoogle Scholar
Weeks, SC (2004) Factors limiting growth in Astragalus michauxii (Sandhills milk-vetch). PhD thesis, North Carolina State University, Raleigh.Google Scholar
Wells, GG (2006) Life history and reproductive biology of Astragalus crassicarpus var. trichocalyx. Masters thesis, Southern Illinois University Edwardsville, Edwardsville, IL.Google Scholar
Willis, CG, Baskin, CC, Baskin, JM, Auld, JR, Venable, DL, Cavender-Bares, J, Donohue, K, Rubio de Casas, R and NESCent Germination Working Group (2014) The evolution of seed dormancy: environmental cues, evolutionary hubs, and diversification of the seed plants. New Phytologist 203, 300–309.CrossRefGoogle ScholarPubMed
Yorgancilar, M and Erisen, S (2011) The effect of thidiazuron (TDZ) on shoot regeneration of Astragalus schizopterus. Journal of Animal and Plant Science 21, 519–524.Google Scholar
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