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A partial purification and characterization of organellar DNA polymerases in germinating maize

Published online by Cambridge University Press:  19 September 2008

Jorge M. Vázquez-Ramos  and
Gloria Martínez
Jorge M. Vázquez-Ramos*
Affiliation:
Departamento de Bioquimica, Conjunto E, Facultad de Quimica, UNAMAvenida Universidad y Copilco, Mexico 04510, D.F. México
Gloria Martínez
Affiliation:
Departamento de Bioquimica, Conjunto E, Facultad de Quimica, UNAMAvenida Universidad y Copilco, Mexico 04510, D.F. México
*
* Correspondence
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Abstract

DNA synthesis in organelles seems to be an early event during maize germination. Mitochondria and plastids from maize embryo axes have been separated using differential and gradient centrifugations and DNA polymerase activity has been studied in extracts from each organelle. It has been found that the enzymes from both organelles show some differences from other plant organellar polymerases in terms of response to specific inhibitors, DNA templates and cations. A comparison of different plant organellar DNA polymerases is presented.

Keywords

DNA polymerasesorganellesseed germinationmaize
Type
Research papers
Information
Seed Science Research , Volume 4 , Issue 1 , March 1994 , pp. 43 - 48
Copyright
Copyright © Cambridge University Press 1994

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