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Patterns of amplified restriction fragment polymorphism in the germination of Festuca hallii seeds

Published online by Cambridge University Press:  07 June 2010

Jie Qiu ,
Yuguang Bai ,
Yong-Bi Fu  and
John F. Wilmshurst
Jie Qiu
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SKS7N 5A8, Canada
Yuguang Bai*
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SKS7N 5A8, Canada
Yong-Bi Fu
Affiliation:
Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SKS7N 0X2, Canada
John F. Wilmshurst
Affiliation:
Jasper National Parks of Canada, Parks Canada, PO Box 10, Jasper, ABT0E 1E0, Canada
*
*Correspondence Fax: +1 306 966 5015 Email: yuguang.bai@usask.ca
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Abstract

Timing of seed germination influences plant lifetime fitness and can affect the ability of plant populations to colonize and persist in changing environments. However, the genetic variation of the seed germination response remains poorly understood. The amplified restriction fragment polymorphism (AFLP) technique was applied to characterize the genetic variation of germinated seeds collected from three Festuca hallii populations in the Canadian prairie. Three subpopulations with early, intermediate and late germination were identified from each population, based on germination tests at 10, 15 and 20°C in controlled growth chambers. Three AFLP primer pairs were employed to screen a total of 540 assayed seedling samples and 188 polymorphic AFLP bands were scored for each sample. None of the assayed AFLP bands were significantly associated with seed germination, but marked differences in estimates of mean band frequency were observed for various groups of germinating seeds under different test temperatures. Partitioning of the total AFLP variation showed that 5.9% AFLP variation was present among seeds of the three populations, 0.3% among seeds of three germination subpopulations, and 0.5% among seeds grouped for germination temperature. Genetic differentiation was significant among 27 groups of seeds representing population, germination timing and test temperature. Subpopulations with early and intermediate germination shared similar genetic backgrounds and were genetically differentiated from the late germination subpopulation. These results indicate that seed genotypes respond slightly differently to environmental variation, resulting in significant but weak genetic differentiation in the germination of F. hallii seeds. Implications for plant establishment and fescue restoration are discussed.

Keywords

AFLPFestuca halliigenetic variationgermination temperaturegermination timing
Type
Research Article
Information
Seed Science Research , Volume 20 , Issue 3 , September 2010 , pp. 153 - 161
Copyright
Copyright © Cambridge University Press 2010

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References

Aiken, S.G., Dallwitz, M.J., McJannet, C.L. and Consaul, L.L. (1996 onwards) Festuca of North America: descriptions, illustrations, identification, and information retrieval. Version 19 October 2005. Available athttp://delta-intkey.com (accessed 5 February 2009).Google Scholar
Aiken, S.G., Dallwitz, M.J., McJannet, C.L. and Consaul, L.L. (1997) Biodiversity among Festuca (Poaceae) in North America: diagnostic evidence from DELTA and clustering programs, and an INTKEY package for interactive, illustrated identification and information retrieval. Canadian Journal of Botany 75, 1527–1555.CrossRefGoogle Scholar
Anderson, D.G. (2006) Festuca hallii (Vasey) Piper (Hall's fescue): a technical conservation assessment (online).USDA Forest Service, Rocky Mountain Region. Available athttp://www.fs.fed.us/r2/projects/scp/assessments/festucahallii.pdf (accessed 5 February 2009).Google Scholar
Bewley, J.D. (1997) Seed germination and dormancy. Plant Cell 9, 1055–1066.CrossRefGoogle ScholarPubMed
Boyd, E.W., Dorn, L.A., Weinig, C. and Schmitt, J. (2007) Maternal effects and germination timing mediate the expression of winter and spring annual life histories in Arabidopsis thaliana. International Journal of Plant Science 168, 205–214.CrossRefGoogle Scholar
Cabin, R.J., Evans, A.S. and Mitchell, R.J. (1997) Genetic effects of germination timing and environment: an experimental investigation. Evolution 51, 1427–1434.CrossRefGoogle ScholarPubMed
Cabin, R.J., Mitchell, R.J.andMarshall, D.L. (1998) Do surface plant and soil seed bank populations differ genetically? A multipopulation study of the desert mustard Lesquerella fendleri (Brassicaceae). American Journal of Botany 85, 1098–1109.CrossRefGoogle ScholarPubMed
Clerkx, E.J., El-Lithy, M.E., Vierling, E., Ruys, G.J., Blankestijn-De Vries, H., Groot, S.P., Vreugdenhil, D. and Koornneef, M. (2004) Analysis of natural allelic variation of Arabidopsis seed germination and seed longevity traits between the accessions Landsberg erecta and Shakdara, using a new recombinant inbred line population. Plant Physiology 135, 432–443.CrossRefGoogle ScholarPubMed
Donohue, K. (2002) Germination timing influences natural selection on life-history characters in Arabidopsis thaliana. Ecology 83, 1006–1016.CrossRefGoogle Scholar
Donohue, K. (2005) Seeds and seasons: interpreting germination timing in the field. Seed Science Research 15, 175–187.CrossRefGoogle Scholar
Donohue, K., Dorn, L., Griffith, C., Kim, E., Aguilera, A., Polisetty, C.R. and Schmitt, J. (2005a) The evolutionary ecology of seed germination of Arabidopsis thaliana: variable natural selection on germination timing. Evolution 59, 758–770.Google ScholarPubMed
Donohue, K., Dorn, L., Griffith, C., Kim, E., Aguilera, A., Polisetty, C.R. and Schmitt, J. (2005b) Niche construction through germination cueing: life-history responses to timing of germination in Arabidopsis thaliana. Evolution 59, 771–785.Google ScholarPubMed
Donohue, K., Dorn, L., Griffith, C., Kim, E., Aguilera, A., Polisetty, C.R. and Schmitt, J. (2005c) Environmental and genetic influences on the germination of Arabidopsis thaliana in the field. Evolution 59, 740–757.Google ScholarPubMed
Excoffier, L., Smouse, P.E. and Quattro, J.M. (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479–491.CrossRefGoogle ScholarPubMed
Fu, Y.-B., Coulman, B.E., Ferdinadez, Y.S.N., Cayouette, J. and Peterson, P.M. (2005a) Genetic diversity of fringed brome (Bromus ciliatus) as determined by amplified fragment length polymorphism. Canadian Journal of Botany 83, 1322–1328.CrossRefGoogle Scholar
Fu, Y.B., Thompson, D., Willms, W. and Mackay, M. (2005b) Long-term grazing effects on genetic variability in mountain rough fescue. Rangeland Ecology and Management 58, 637–642.CrossRefGoogle Scholar
Garcia-Huidobro, J., Monteith, J.L. and Squire, G.R. (1982) Time, temperature and germination of pearl millet (Pennisetum typhoides S. & H.). I. Constant temperature. Journal of Experimental Botany 33, 288–296.CrossRefGoogle Scholar
Holdsworth, M.J., Finch-Savage, W.E. and Grappin, P. (2008) Post-genomics dissection of seed dormancy and germination. Trends in Plant Science 13, 7–13.CrossRefGoogle ScholarPubMed
Huff, D.R., Quinn, J.A., Higgins, B. and Palazzo, A.J. (1998) Random amplified polymorphic DNA (RAPD) variation among native little bluestem [Schizachyrium scoparium (Michx.) Nash] populations from sites of high and low fertility in forest and grassland biomes. Molecular Ecology 7, 1591–1598.CrossRefGoogle Scholar
Johnston, A. and MacDonald, M.D. (1967) Floral initiation and seed production in Festuca scabrella Torr. Canadian Journal of Plant Science 47, 577–583.CrossRefGoogle Scholar
Kebreab, E. and Murdoch, A.J. (1999a) A model of the effects of a wide range of constant and alternating temperatures on seed germination of four Orobanche species. Annals of Botany 84, 549–557.CrossRefGoogle Scholar
Kebreab, E. and Murdoch, A.J. (1999b) Modelling the effects of water stress and temperature on germination rate of Orobanche aegyptiaca seeds. Journal of Experimental Botany 50, 655–664.CrossRefGoogle Scholar
Lacey, E.P. (1996) Parental effects in Plantago lanceolata L. I.: a growth chamber experiment to examine pre- and postzygotic temperature effects. Evolution 50, 865–878.CrossRefGoogle Scholar
Larson, S.R., Waldron, B.L., Monsen, S.B., St John, L., Palazzo, A.J., McCracken, C.L. and Harrison, R. (2001a) AFLP variation in agamospermous and dioecious bluegrasses of western North America. Crop Science 41, 1300–1305.CrossRefGoogle Scholar
Larson, S.R., Cartier, E., McCracken, C.L. and Dyer, D. (2001b) Mode of reproduction and amplified fragment length polymorphism variation in purple needlegrass (Nassella pulchra): utilization of natural germplasm sources. Molecular Ecology 10, 1165–1177.CrossRefGoogle ScholarPubMed
Liptay, A. and Davidson, D. (1971) Coleoptile growth: variation in elongation patterns of individual coleoptiles. Annals of Botany 35, 991–1002.CrossRefGoogle Scholar
Luzuriaga, A.L., Escudero, A. and Perez-Garcia, F. (2006) Environmental maternal effects on seed morphology and germination in Sinapis arvensis (Cruciferae). Weed Research 46, 163–174.CrossRefGoogle Scholar
Mandak, B., Bimova, K. and Plackova, I. (2006) Genetic structure of experimental populations and reproductive fitness in a heterocarpic plant Atriplex tatarica (Chenopodiaceae). American Journal of Botany 93, 1640–1649.CrossRefGoogle Scholar
Marshall, B., Dunlop, G., Ramsay, G. and Squire, G.R. (2000) Temperature-dependent germination traits in oilseed rape associated with 5′-anchored simple sequence repeat PCR polymorphisms. Journal of Experimental Botany 51, 2075–2084.CrossRefGoogle ScholarPubMed
Pendleton, R.L. and Meyer, S.E. (2004) Habitat-correlated variation in blackbrush (Coleogyne ramosissima: Rosaceae) seed germination response. Journal of Arid Environments 59, 229–243.CrossRefGoogle Scholar
Qiu, J. (2009) Patterns of genetic variation in Festuca hallii (Vasey) Piper across the Canadian Prairie. PhD thesis, University of Saskatchewan, Canada.Google Scholar
Qiu, J., Bai, Y., Coulman, B. and Romo, J.T. (2006) Using thermal time models to predict seedling emergence of orchardgrass (Dactylis glomerata L.) under alternating temperature regimes. Seed Science Research 16, 261–271.CrossRefGoogle Scholar
Qiu, J., Fu, Y.B., Bai, Y. and Wilmshurst, J.F. (2007) Patterns of amplified restriction fragment polymorphism in natural populations and corresponding seed collections of plains rough fescue (Festuca hallii). Canadian Journal of Botany 85, 484–492.CrossRefGoogle Scholar
Qiu, J., Fu, Y.B., Bai, Y. and Wilmshurst, J.F. (2009) Genetic variation in remnant Festuca hallii populations is weakly differentiated but geographically associated across the Canadian Prairie. Plant Species Biology 24, 156–168.CrossRefGoogle Scholar
Rajjou, L., Gallardo, K., Debeaujon, I., Vandekerckhove, J., Job, C. and Job, D. (2004) The effect of α-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiology 134, 1598–1613.CrossRefGoogle ScholarPubMed
Roach, D.A. and Wulff, R.D. (1987) Maternal effects in plants. Annual Review of Ecology and Systematics 18, 209–235.CrossRefGoogle Scholar
Rohlf, F.J. (1997) NTSYS-pc 2.1. Numerical taxonomy and multivariate analysis system. New York, Exeter Software.Google Scholar
Romo, J.T. (2003) Reintroducing fire for conservation of Fescue Prairie Association remnants in the northern Great Plains. Canadian Field-Naturalist 117, 89–99.CrossRefGoogle Scholar
Romo, J.T., Grilz, P.L., Bubar, C.J. and Young, J.A. (1991) Influences of temperature and water stress on germination of plains rough fescue. Journal of Range Management 44, 75–81.CrossRefGoogle Scholar
Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.Google Scholar
Squire, G.R., Marshall, B., Dunlop, G. and Wright, G. (1997) Genetic basis of rate-temperature characteristics for germination in oilseed rape. Journal of Experimental Botany 48, 869–875.CrossRefGoogle Scholar
Still, D.W. and Bradford, K.J. (1997) Endo-[beta]-mannanase activity from individual tomato endosperm caps and radicle tips in relation to germination rates. Plant Physiology 113, 21–29.CrossRefGoogle Scholar
Still, D.W., Dahal, P. and Bradford, K.J. (1997) A single-seed assay for endo-β-mannanase activity from tomato endosperm and radicle tissues. Plant Physiology 113, 13–20.CrossRefGoogle ScholarPubMed
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van De Lee, T., Hornes, M., Frijters, A., Peleman, J., Kuiper, M. and Zabeau, M. (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407–4414.CrossRefGoogle ScholarPubMed
Wang, R., Bai, Y. and Tanino, K. (2004) Effect of seed size and sub-zero imbibition-temperature on the thermal time model of winterfat (Eurotia lanata (Pursh) Moq.). Environmental and Experimental Botany 51, 183–197.CrossRefGoogle Scholar
Zhang, W. (2008) A comprehensive study on the role of hormones, seed coat and genes during the germination of canola (Brassica napus) seed under adverse environmental conditions. PhD thesis, University of Saskatchewan, Canada.Google Scholar