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Pre-harvest stress cracks in maize (Zea mays L.) kernels as characterized by visual, X-ray and low temperature scanning electron microscopical analysis: effect on kernel quality

Published online by Cambridge University Press:  22 February 2007

Maria Laene Moreira de Carvalho ,
Adriaan C. van Aelst ,
Jan Walter van Eck  and
Folkert A. Hoekstra
Maria Laene Moreira de Carvalho*
Affiliation:
Department of Agriculture, Universidade Federal de Lavras, P.O.Box 37, 37750–000 Lavras, MG, Brazil Laboratories of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The, Netherlands
Adriaan C. van Aelst*
Affiliation:
Plant Cytology and Morphology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The, Netherlands
Jan Walter van Eck*
Affiliation:
DLO-Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA Wageningen, The, Netherlands
Folkert A. Hoekstra*
Affiliation:
Laboratories of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The, Netherlands
*
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
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Abstract

Internal cracks caused by high temperature or excessive moisture during maize (Zea mays L.) kernel development were characterized, and their effects on kernel quality were assessed. Pre-harvest stress cracks are often located near the middle of the kernel along the embryo axis, but they were also detected in other positions, irrespective of the shape of the kernel. X-ray analysis enabled visualisation of stress cracks that are invisible to the human eye and, therefore, gave a better estimate of the percentage of cracks. However, low temperature scanning electron microscopy of the surface of milled kernels revealed small cracks not noticed by visual or X-ray inspection. All kernels tested in this way had a crack of some sort in the endosperm tissue. Cracks were also frequent in the scutellum, but rare in the embryo axis. Endosperm cracks followed the boundary of the starch granules, but did not extend into the pericarp tissue. In contrast to external cracks caused by mechanical impact, pre-harvest internal stress cracks generally are not detrimental to germination and vigour. However, if the crack is located inside or perpendicular to the embryo axis, it may affect the quality of the kernel, probably by impeding nutrient translocation to the embryo.

Keywords

cracksgerminationkernelscanning electron microscopyvigourX-ray analysisZea maysL
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 3 , March 1999 , pp. 227 - 236
Copyright
Copyright © Cambridge University Press 1999

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