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Protein and gene expression patterns of endo-β-mannanase following germination of rice

Published online by Cambridge University Press:  01 September 2008

Yanfang Ren ,
J. Derek Bewley  and
Xiaofeng Wang
Yanfang Ren
Affiliation:
Laboratory of Seed Science and Technology, College of Life Sciences, South China Agricultural University, Guangzhou510642, China
J. Derek Bewley
Affiliation:
Department of Molecular and Cellular Biology, University of Guelph, Guelph, OntarioN1G 2W1, Canada
Xiaofeng Wang*
Affiliation:
Laboratory of Seed Science and Technology, College of Life Sciences, South China Agricultural University, Guangzhou510642, China
*
*Correspondence Fax: (86) 20-85282180xfwang@scau.edu.cn
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Abstract

The rice (Oryza sativa L.) cv. Taichung 65, a japonica subspecies, was used to characterize the isoform, protein and gene expression patterns of endo-β-mannanase during and after seed germination. Activity assays and isoform analyses of whole grains or seed parts (scutellum, aleurone layer and starchy endosperm) revealed that seeds began to express endo-β-mannanase activity at 48 h from the start of imbibition at 25°C, after the completion of germination of most seeds. Three isoforms of endo-β-mannanase (pI 8.86, pI 8.92 and pI 8.98) were detected in the aleurone layer and starchy endosperm, but only two (pI 8.86 and pI 8.92) were present in the scutellum. The endo-β-mannanase in the starchy endosperm was mainly from the aleurone layer. Western blot analysis, using a tomato anti-endo-β-mannanase antibody, indicated that an endo-β-mannanase protein was present in an inactive form in dry grains. The amount of this protein decreased in the scutellum, but increased in the aleurone layer during and after germination. Thus, the increase in endo-β-mannanase activity in rice grains may be due to the activation of extant proteins and/or the de novo synthesis of the enzyme. Northern blot analysis showed that four putative rice endo-β-mannanase genes (OsMAN1, OsMAN2, OsMAN6 and OsMANP) were expressed in germinating and germinated rice grains. However, OsMANP was not expressed in the scutellum. The amount of OsMAN6 mRNA decreased after the completion of germination and paralleled the decline in endo-β-mannanase protein. In the aleurone layer, the increase of OsMAN2, OsMAN6 and OsMANP mRNA was prior to the increase of endo-β-mannanase protein.

Keywords

aleurone layerendo-β-mannanaseendospermgerminationOryza sativascutellum
Type
Research Opinion
Information
Seed Science Research , Volume 18 , Issue 3 , September 2008 , pp. 139 - 149
Copyright
Copyright © Cambridge University Press 2008

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References

Bewley, J.D. (1997) Breaking down the walls – a role for endo-β-mannanase in release from seed dormancy? Trends in Plant Science 2, 464–469.CrossRefGoogle Scholar
Bewley, J.D., Burton, R.A., Morohashi, Y. and Fincher, G.B. (1997) Molecular cloning of a cDNA encoding a (1,4)-β-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum). Planta 203, 454–459.CrossRefGoogle Scholar
Bewley, J.D., Banik, M., Bourgault, R., Feurtado, J.A., Toorop, P. and Hilhorst, H.W.M. (2000) Endo-β-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening. Journal of Experimental Botany 51, 529–538.CrossRefGoogle ScholarPubMed
Bourgault, R. and Bewley, J.D. (2002) Gel diffusion assays for endo-β-mannanase and pectin methylesterase can underestimate enzyme activity due to proteolytic degradation: a remedy. Analytical Biochemistry 300, 87–93.CrossRefGoogle ScholarPubMed
DeMason, D.A., Sexton, R., Gorman, M. and Reid, J.S.G. (1985) Structure and biochemistry of endosperm breakdown in date palm (Phoenix dactylifera L.) seeds. Protoplasma 126, 159–167.CrossRefGoogle Scholar
Dirk, L.M.A., Griffen, A.M., Downie, B. and Bewley, J.D. (1995) Multiple isozymes of endo-β-D-mannanase in dry and imbibed seeds. Phytochemistry 40, 1045–1056.CrossRefGoogle Scholar
Dulson, J. and Bewley, J.D. (1989) Mannanase from Lactuca sativa: metabolic requirements for production and partial purification. Phytochemistry 28, 363–369.CrossRefGoogle Scholar
Gong, X. (2007) Seed germination and the roles of GA-regulated genes: endo-β-mannanase and GAMYB. PhD thesis, University of Guelph, Canada.Google Scholar
Gong, X. and Bewley, J.D. (2007) Sorting out the LeMans: endo-β-mannanase genes and their encoded proteins in tomato. Seed Science Research 17, 143–154.CrossRefGoogle Scholar
Gong, X., Bassel, G.W., Wang, A.X., Greenwood, J. and Bewley, J.D. (2005) The emergence of embryos from hard seeds is related to the structure of the cell walls of the micropylar endosperm, and not to endo-β-mannanase activity. Annals of Botany 96, 1165–1173.CrossRefGoogle Scholar
Groot, S.P.C., Kieliszewska-Rokicka, B., Vermeer, E. and Karssen, C.M. (1988) Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion. Planta 174, 500–504.CrossRefGoogle ScholarPubMed
Halmer, P. (1989) De novo synthesis of mannanase by the endosperm of Lactuca sativa. Phytochemistry 28, 371–377.CrossRefGoogle Scholar
Halmer, P. and Bewley, J.D. (1979) Mannanase production by the lettuce endosperm: control by the embryo. Planta 144, 333–340.CrossRefGoogle ScholarPubMed
Homrichhausen, T.M., Hewitt, J.R. and Nonogaki, H. (2003) Endo-β-mannanase activity is associated with the completion of embryogenesis in imbibed carrot (Daucus carota L.) seeds. Seed Science Research 13, 219–227.CrossRefGoogle Scholar
Hrmova, M., Burton, R.A., Biely, P., Lahnstein, J. and Fincher, G.B. (2006) Hydrolysis of (1,4)-β-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-β-D-mannan endohydrolase and β-D-mannosidase. Biochemical Journal 399, 77–90.CrossRefGoogle Scholar
Malek, L. and Bewley, J.D. (1991) Endo-β-mannanase activity and reserve mobilization in excised endosperms of fenugreek is affected by volume of incubation and abscisic acid. Seed Science Research 1, 45–49.CrossRefGoogle Scholar
Marraccini, P., Rogers, W.J., Allard, C., Andre, M.L., Gaillet, V., Lacoste, N., Lausanne, F. and Michaux, S. (2001) Molecular and biochemical characterization of endo-β-mannanases from germinating coffee (Coffea arabica) grains. Planta 213, 296–308.CrossRefGoogle ScholarPubMed
Nomaguchi, M., Nonogaki, H. and Morohashi, Y. (1995) Development of galactomannan-hydrolyzing activity in the micropylar endosperm tip of tomato seed prior to germination. Physiologia Plantarum 94, 105–109.CrossRefGoogle Scholar
Nonogaki, H. and Morohashi, Y. (1996) An endo-β-mannanase develops exclusively in the micropylar endosperm of tomato seeds prior to radicle emergence. Physiologia Plantarum 110, 555–559.CrossRefGoogle ScholarPubMed
Nonogaki, H. and Morohashi, Y. (1999) Temporal and spatial pattern of the development of endo-β-mannanase activity in germinating and germinated lettuce seeds. Journal of Experimental Botany 50, 1307–1313.Google Scholar
Nonogaki, H., Matsushima, H. and Morohashi, Y. (1992) Galactomannan hydrolyzing activity develops during priming in the micropylar endosperm tip of tomato seeds. Physiologia Plantarum 85, 167–172.CrossRefGoogle Scholar
Nonogaki, H., Nomaguchi, M. and Morohashi, Y. (1995) Endo-β-mannanase in the endosperm of germinated tomato seeds. Physiologia Plantarum 94, 328–334.CrossRefGoogle Scholar
Nonogaki, H., Nomaguchi, M., Morohashi, Y. and Matsushima, H. (1998) Development and localization of endo-β-mannanase in the embryo of germinating and germinated tomato seeds. Journal of Experimental Botany 49, 1501–1507.Google Scholar
Nonogaki, H., Gee, O.H. and Bradford, K.J. (2000) A germination-specific endo-β-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds. Plant Physiology 123, 1235–1246.CrossRefGoogle ScholarPubMed
Sánchez, R.A. and de Miguel, L. (1997) Phytochrome promotion of mannan-degrading enzyme activities in the micropylar endosperm of Datura ferox seeds requires the presence of the embryo and gibberellin synthesis. Seed Science Research 7, 27–33.CrossRefGoogle Scholar
Sánchez, R., Sunell, L., Labavitch, J. and Bonner, B. (1990) Changes in endosperm cell walls of two Datura species before radicle protrusion. Plant Physiology 93, 89–97.CrossRefGoogle ScholarPubMed
Shen, W.B., Wang, R., Wang, Y.H., Zheng, T.Q. and Wan, J.M. (2003) A novel method of extracting total RNA from rice embryo samples. Hereditas 25, 208–210.Google ScholarPubMed
Thévenot, C., Simond-Côte, E. and Daussant, J. (1991) Contribution of aleurone layer and scutellum to α-amylase synthesis and secretion in wheat and rice grains. Physiologia Plantarum 82, 249–256.CrossRefGoogle Scholar
Toorop, P.E., Bewley, J.D. and Hilhorst, H.W.M. (1996) Endo-β-mannanase isoforms are present in the endosperm and embryo of tomato seeds, but are not essentially linked to the completion of germination. Planta 200, 153–158.CrossRefGoogle Scholar
Voigt, B. and Bewley, J.D. (1996) Developing tomato seeds when removed from the fruit tissue produce multiple forms of germinative and post-germinative endo-β-mannanase. Responses to desiccation, abscisic acid, and osmoticum. Planta 200, 71–77.CrossRefGoogle Scholar
Wang, A.X., Li, J.R. and Bewley, J.D. (2004) Molecular cloning and characterization of an endo-β-mannanase gene expressed in the lettuce endosperm following radicle emergence. Seed Science Research 14, 267–276.CrossRefGoogle Scholar
Wang, A., Wang, X., Ren, Y., Gong, X. and Bewley, J.D. (2005) Endo-β-mannanase and β-mannosidase activities in rice grains during and following germination, and the influence of gibberellin and abscisic acid. Seed Science Research 15, 219–227.CrossRefGoogle Scholar
Yuan, J.S., Yang, X., Lai, J., Lin, H., Cheng, Z.M., Nonogaki, H. and Chen, F. (2007) The endo-β-mannanase gene families in Arabidopsis, rice, and poplar. Functional and Integrative Genomics 7, 1–16.CrossRefGoogle ScholarPubMed
Zhao, T., Yan, M., Lu, Y.P., Yang, F., Huang, J. and Wang, X. (2005) Genetic purity testing of two-line hybrid rice seeds by ultrathin-layer isoelectric focusing of proteins. Seed Science and Technology 33, 45–52.CrossRefGoogle Scholar