Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-10T13:23:23.115Z Has data issue: false hasContentIssue false
  • English
  • Français

Quantitative estimation of seed desiccation sensitivity using a quantal response model: application to nine species of the genus Coffea L.

Published online by Cambridge University Press:  22 February 2007

Stéphane Dussert ,
Nathalie Chabrillange ,
Florent Engelmann  and
Serge Hamon
Stéphane Dussert*
Affiliation:
ORSTOM, GeneTrop, BP 5045, 34032 Montpellier Cedex 1, France
Nathalie Chabrillange
Affiliation:
ORSTOM, GeneTrop, BP 5045, 34032 Montpellier Cedex 1, France
Florent Engelmann
Affiliation:
IPGRI, Via delle Sette Chiese 142, 00145 Rome, Italy
Serge Hamon
Affiliation:
ORSTOM, GeneTrop, BP 5045, 34032 Montpellier Cedex 1, France
*
*Correspondence Fax: +33 4 67 54 78 00 Email: dussert@orstom.fr
Get access Rights & Permissions [Opens in a new window]

Abstract

Seed desiccation sensitivity was studied in nine species of the genus Coffeaby measuring seed viability after equilibration over various saturated salt solutions. A quantal response model based on the logistic distribution was developed in order to describe the typical S-shaped patterns observed. The closeness of fit of the desiccation sensitivity model was shown, and the assumption that seed desiccation sensitivity follows a continuous distribution within species was verified. For each species, the water content at which 50% of initial viability was reached, WC50, and a specific parameter describing the intra-specific variability, β, were calculated using a non-linear regression. A simplified water sorption model was developed which allowed easy calculation of water activity and water potential corresponding to WC50 (aw50 and Ψ50) for relative humidities ranging between 10 and 100%. Distribution of WC50and Ψ50 (or aw50) in the genus Coffea was homogeneous within the following intervals: from 0.05 to 0.38 g H2O.g−1dw for WC50 and from −168 to −11 MPa for Ψ50. Different classifications of the coffee species studied as regards to their desiccation sensitivity were obtained depending on whether WC50 or Ψ50was used for classification. The continuum for desiccation sensitivity observed within the nine species studied confirmed that coffee is an appropriate material for studying desiccation sensitivity.

Keywords

Coffeaseeddesiccation sensitivity modelsorption isotherm
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 2 , February 1999 , pp. 135 - 144
Copyright
Copyright © Cambridge University Press 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdelnour-Esquivel, A., Villalobos, V. and Engelmann, F. (1992) Cryopreservation of zygotic embryos of Coffea spp. Cryo-Letters 13, 297–302.Google Scholar
Berjak, P. and Pammenter, N.W. (1994) Recalcitrance is not an all-or-nothing situation. Seed Science Research 4, 263–264.CrossRefGoogle Scholar
Berjak, P. and Pammenter, N.W. (1997) Progress in the understanding and manipulation of desiccationsensitive (recalcitrant) seeds. pp 689–703 in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, The Netherlands, Kluwer Academic Publishers.CrossRefGoogle Scholar
Budavari, S., O'Neil, M.J., Smith, A. and Heckelman, P.E. (1989) The Merck Index. Rahway, NJ, USA, Merck & Co. Inc.Google Scholar
Dickie, J.B., May, K., Morris, S.V.A. and Titley, S.E. (1991) The effects of desiccation on seed survival in Acer platanoides L. and Acer pseudoplatanus L. Seed Science Research 1, 149–162.CrossRefGoogle Scholar
Dussert, S., Chabrillange, N., Engelmann, F., Anthony, F., Louarn, J. and Hamon, S. (1998) Cryopreservation of seeds of four coffee species (Coffea arabica, C. costatifructa, C. racemosa and C. sessiliflora): importance of water content and cooling rate. Seed Science Research 8, 9–15.CrossRefGoogle Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1990) An intermediate category of seed storage behaviour? I. Coffee. Journal of Experimental Botany 41, 1167–1174.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1988) Recalcitrance – a current assessment. Seed Science and Technology 16, 155–166.Google Scholar
Finch-Savage, W.E. (1992) Embryo water status and survival in the recalcitrant species Quercus robur L.: evidence for a critical moisture content. Journal of Experimental Botany 43, 663–669.CrossRefGoogle Scholar
Finney, D.J. (1978) Statistical methods in biological assay. (3rd edition) New York, Macmillan.Google Scholar
Hong, T.D. and Ellis, R.H. (1995) Interspecific variation in seed storage behaviour within two genera – Coffea and Citrus. Seed Science and Technology 23, 165–181.Google Scholar
Hong, T.D. and Ellis, R.H. (1996) A protocol to determine seed storage behaviour. Rome, Italy, International Plant Genetic Resources Institute.Google Scholar
Kovach, D.A. and Bradford, K.J. (1992) Imbibitional damage and desiccation tolerance of wild rice (Zizania palustris) seeds. Journal of Experimental Botany 43, 747–757.CrossRefGoogle Scholar
Normah, M.N. and Vengadasalam, M. (1992) Effect of moisture content on cryopreservation of Coffea and Vigna seeds and embryos. Cryo-Letters 13, 199–208.Google Scholar
Pritchard, H.W. (1991) Water potential and embryonic axis viability in recalcitrant seeds of Quercus rubra. Annals of Botany 67, 43–49.CrossRefGoogle Scholar
Probert, R.J. and Longley, P.L. (1989) Recalcitrant seed storage physiology in three aquatic grasses (Zizania palustris, Spartina anglica and Porteresia coarcata). Annals of Botany 63, 53–63.CrossRefGoogle Scholar
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Tompsett, P.B. (1982) The effect of desiccation on the longevity of seeds of Araucaria hunsteinii and A. cunninghamii. Annals of Botany 50, 693–704.CrossRefGoogle Scholar
Tompsett, P.B. (1987) Desiccation and storage studies in Dipterocarpus species. Annals of Applied Biology 110, 371–379.CrossRefGoogle Scholar
Vertucci, C.W. and Farrant, J.M. (1995) Acquisition and loss of desiccation tolerance. pp 237–271 in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Vertucci, C.W. and Leopold, A.C. (1987) Water binding in legume seeds. Plant Physiology 85, 224–231.CrossRefGoogle ScholarPubMed