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Recalcitrant and orthodox Spartina seeds or isolated embryos exhibit similar leachate patterns immediately following desiccation

Published online by Cambridge University Press:  25 March 2015

James H. Chappell Jr ,
Yi Wang  and
Marc Alan Cohn
James H. Chappell Jr
Affiliation:
Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
Yi Wang
Affiliation:
Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
Marc Alan Cohn*
Affiliation:
Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
*
*Correspondence E-mail: mcohn@lsu.edu
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Abstract

Solute leakage is used as an indicator of membrane damage during desiccation of recalcitrant seeds. We re-examined this phenomenon and its utility by comparing recalcitrant Spartina alterniflora and orthodox Spartina pectinata seeds, as well as drying isolated embryos or embryos isolated after whole-seed drying. During drying, intact seed leakage (electrical conductivity and absorbance at 280 nm) was independent of seed moisture content, dormant/non-dormant state or desiccation tolerance. Embryos from both Spartina species, isolated after drying within the intact seed, exhibited increased leakage, especially below 40% (dry weight basis, DWB) seed moisture, the critical water content for viability loss in Spartina alterniflora. When isolated embryos were dried, the pattern and extent of increased solute leakage were similar in both Spartina species, even though S. pectinata embryos were >95% viable and S. alterniflora embryos were < 20% viable. We conclude that increased solute leakage is an artefact of embryo excision and not an accurate indicator of desiccation damage to recalcitrant Spartina alterniflora seeds.

Keywords

desiccation toleranceelectrical conductivitymembrane damagerecalcitrant seedssolute leakageSpartinaviability
Type
Research Papers
Information
Seed Science Research , Volume 25 , Issue 3 , September 2015 , pp. 247 - 254
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Copyright
Copyright © Cambridge University Press 2015 

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