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The re-establishment of desiccation tolerance in germinated radicles of Medicago truncatula Gaertn. seeds

Published online by Cambridge University Press:  22 February 2007

Julia Buitink ,
Benoit Ly Vu ,
Pascale Satour  and
Olivier Leprince
Julia Buitink*
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
Benoit Ly Vu
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
Pascale Satour
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
Olivier Leprince
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
*
*Correspondence Fax: +33 241 739309 Email: julia.buitink@angers.inra.fr
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Abstract

Germinated seeds of Medicago truncatula Gaertn. with a protruded radicle length of 2.7 mm did not survive drying below 0.2 g H2O g–1 dw, as indicated by vital stain assays and the absence of growth resumption after rehydration. The re-establishment of desiccation tolerance was achieved using an osmotic treatment with polyethylene glycol (PEG), combined with a cold treatment. The ability to regain desiccation tolerance after germination was restricted to a period of growth characterized by radicle lengths between 1 and 3 mm. After PEG treatment of germinated seeds with 2.7 mm long radicles at –1.7 MPa at 10°C for 3 d and subsequent drying to 0.04 g H2O g–1 dw, 90% survived and developed into normal seedlings after rehydration. Desiccation tolerance could also be re-established in excised radicles, demonstrating that cotyledons were not essential for this process. Upon PEG incubation, sucrose accumulated rapidly prior to the re-establishment of desiccation tolerance in germinated radicles, regardless of the presence of cotyledons. Induction of MtDHN (a dehydrin) gene expression was correlated with the re-establishment of desiccation tolerance. Furthermore, the PEG-induced expression of MtDHN was repressed when fluridone was added to the PEG solution.

Keywords

Medicago truncatuladesiccation tolerancesucrosedehydrinsgerminationosmotic shockabscisic acid
Type
Research Article
Information
Seed Science Research , Volume 13 , Issue 4 , December 2003 , pp. 273 - 286
Copyright
Copyright © Cambridge University Press 2003

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References

Amuti, K.S. and Pollard, C.J. (1977) Soluble carbohydrates of dryand developing seeds. Phytochemistry 16, 529–532.CrossRefGoogle Scholar
Bell, C.J., Dixon, R.A., Farmer, A.D., Flores, R., Inman, J., Gonzales, R.A., Harrison, M.J., Paiva, N.L., Scott, A.D., Weller, J.W. and May, G.D. (2001) The Medicago genome initiative: a model legume database. Nucleic Acids Research 29, 114–117.CrossRefGoogle Scholar
Black, M., Corbineau, F., Gee, H. and Côme, D. (1999) Water content, raffinose,and dehydrins in the induction of desiccation tolerance in immature wheat embryos. Plant Physiology 120, 463–471.CrossRefGoogle ScholarPubMed
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteinsand sugars in desiccation tolerance of developing seeds. Plant Physiology 100, 225–230.CrossRefGoogle Scholar
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1995) Desiccation tolerance in developing soybean seeds – the role of stress proteins. Physiologia Plantarum 93, 630–638.CrossRefGoogle Scholar
Bruggink, T. and van der Toorn, P. (1995) Induction of desiccation tolerance in germinated seeds. Seed Science Research 5, 1–4.CrossRefGoogle Scholar
Buitink, J., Leprince, O. and Hoekstra, F.A. (2000) Dehydration-induced redistribution of amphiphilic molecules between cytoplasmand lipids is associated with desiccation tolerance in seeds. Plant Physiology 124, 1413–1426.CrossRefGoogle Scholar
Buitink, J., Hoekstra, F.A. and Leprince, O. (2002) Biochemistryand biophysics of tolerance systems. pp. 293–318in Black, M.;Pritchard, H.W. (Eds) Desiccationand survival in plants. Wallingford, CABI Publishing.Google Scholar
Butler, W.M. and Cuming, A.C. (1993) Differential molecular responses to abscisic acidand osmotic stress in viviparous maize embryos. Planta 189, 47–54.CrossRefGoogle Scholar
Campbell, S.A. and Close, T.J. (1997) Dehydrins: genes, proteinsand associations with phenotypic traits. New Phytologist 137, 61–74.CrossRefGoogle Scholar
Close, T.J. (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiologia Plantarum 97, 795–803.CrossRefGoogle Scholar
Cook, D.R. (1999) Medicago truncatula – a model in the making! Current Opinion in Plant Biology 2, 301–304.CrossRefGoogle Scholar
Finkelstein, R.R. and Lynch, T.J. (2000) The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell 12, 599–609.CrossRefGoogle ScholarPubMed
Golovina, E.A., Hoekstra, F.A. and Van Aelst, A.C. (2001) The competence to acquire cellular desiccation-tolerance is independent of seed morphological development. Journal of Experimental Botany 52, 1015–1027.CrossRefGoogle ScholarPubMed
Hoekstra, F.A., Golovina, E.A. and Buitink, J. (2001) Mechanisms of plant desiccation tolerance. Trends in Plant Science 6, 431–438.CrossRefGoogle ScholarPubMed
Horváth, I., Glatz, A., Varvasovszki, V., Török, Z., Páli, T., Balogh, G., Kovács, E., Nádasdi, L., Benko, S., Joó, F. and Vígh, L. (1998) Membrane physical state controls the signaling mechanism of the heat shock response in Synechocystis PCC 6803: Identification of hsp17 as a ‘fluidity gene’. Proceedings of the National Academy of Sciences, USA 95, 3513–3518.CrossRefGoogle ScholarPubMed
Huang, Z., Fasco, M.J. and Kaminsky, L.S. (1996) Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. BioTechniques 20, 1012–1020.CrossRefGoogle ScholarPubMed
Ingram, J. and Bartels, D. (1996) The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiologyand Plant Molecular Biology 47, 377–403.CrossRefGoogle ScholarPubMed
Kermode, A.R. (1995) Regulatory mechanisms in the transition from seed development to germination: interactions between the embryoand the seed environment. pp. 273–332in Kigel, J.;Galili, G. (Eds) Seed developmentand germination. New York, Marcel Dekker.Google Scholar
Kermode, A.R. (1997) Approaches to elucidate the basis of desiccation-tolerance in seeds. Seed Science Research 7, 75–95.CrossRefGoogle Scholar
Knight, H. and Knight, M.R. (2001) Abiotic stress signalling pathways: specificityand cross-talk. Trends in Plant Science 6, 262–267.CrossRefGoogle Scholar
Koster, K.L. and Leopold, A.C. (1988) Sugarsand desiccation tolerance in seeds. Plant Physiology 88, 829–832.CrossRefGoogle Scholar
Leprince, O., Harren, F.J.M., Buitink., J., Alberda, M. and Hoekstra, F.A. (2000) Metabolic dysfunctionand unabated respiration precede the loss of membrane integrity during dehydration of germinating radicles. Plant Physiology 122, 597–608.CrossRefGoogle Scholar
Lopez-Molina, L., Mongrand, S. and Chua, N.-H. (2001) A postgermination developmental arrest checkpoint is mediated by abscisic acidand requires the ABI5 transcription factor in Arabidopsis. Proceedings of the National Academy of Sciences, USA 98, 4782–4787.CrossRefGoogle Scholar
McKee, J.M.T. and Finch-Savage, W.E. (1989) The effect of abscisic acid on the growthand storage of germinating rape (Brassica napus L.) seed dried following selection on the basis of a newly-emerged radicle. Plant Growth Regulation 8, 77–83.CrossRefGoogle Scholar
Michel, B.E. and Kaufmann, M.R. (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology 51, 914–916.CrossRefGoogle ScholarPubMed
Ooms, J.J.J., van der Veen, R. and Karssen, C.M. (1994) Abscisic acidand osmotic stress or slow drying independently induce desiccation tolerance in mutant seeds of Arabidopsis thaliana. Physiologia Plantarum 92, 506–510.CrossRefGoogle Scholar
Raz, V., Bergervoet, J.H.W. and Koornneef, M. (2001) Sequential steps for developmental arrest in Arabidopsis seeds. Development 128, 243–252.CrossRefGoogle ScholarPubMed
Reisdorph, N.A. and Koster, K.L. (1999) Progressive loss of desiccation tolerance in germinating pea (Pisum sativum) seeds. Physiologia Plantarum 105, 266–271.CrossRefGoogle Scholar
Roberton, M. and Chandler, P.M. (1992) Pea dehydrins: identification, characterizationand expression. Plant Molecular Biology 19, 1031–1044.CrossRefGoogle Scholar
Sambrook, J. and Russell, D.W. (2001) Molecular cloning: A laboratory manual (3rd edition). Cold Spring Harbor, Cold Spring Harbor Laboratory Press.Google Scholar
Shinozaki, K., Yamaguchi-Shinozaki, K. (2000) Molecular responses to dehydrationand low temperature: differencesand cross-talk between two stress signaling pathways. Current Opinion in Plant Biology 3, 217–223.CrossRefGoogle Scholar
Verwoerd, T.C., Dekker, B.M.M. and Hoekema, A. (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17, 2362.CrossRefGoogle ScholarPubMed
Wehmeyer, N. and Vierling, E. (2000) The expression of small heat shock proteins in seeds responds to discrete developmental signalsand suggests a general protective role in desiccation tolerance. Plant Physiology 122, 1099–1108.CrossRefGoogle Scholar
Whalley, W.R., Lipiec, J., Finch-Savage, W.E., Cope, R.E., Clark, L.J. and Rowse, H.R. (2001) Water stress can induce quiescence in newly-germinated onion (Allium cepa L.) seedlings. Journal of Experimental Botany 52, 1129–1133.CrossRefGoogle ScholarPubMed