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Relation between protein composition and germination capacity of sunflower seeds

Published online by Cambridge University Press:  19 September 2008

C. Reuzeau ,
D. Goffner  and
G. Cavalié
C. Reuzeau*
Affiliation:
University Paul Sabatier, Plant Physiology Institute, URA CNRS no 1457, 118, route de Narbonne, 31062 Toulouse, France
D. Goffner
Affiliation:
University Paul Sabatier, Plant Physiology Institute, URA CNRS no 1457, 118, route de Narbonne, 31062 Toulouse, France
G. Cavalié
Affiliation:
University Paul Sabatier, Plant Physiology Institute, URA CNRS no 1457, 118, route de Narbonne, 31062 Toulouse, France
*
* Correspondence
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Abstract

Protein composition of sunflower seeds (Helianthus annuus L.) was analysed to identify a possible marker for germination capacity and to evaluate seed viability precociously after harvest and after natural ageing during storage. Electrophoresis on 2-D gels was used to compare total soluble and in vivo synthesized proteins in two seed lots showing different germination capacities. Differences were observed to a greater extent in in vivo protein synthesis profiles than in total soluble proteins. Inhibition of [35S]methionine uptake and incorporation in seeds with low germinability was also observed. The precise relationship between protein composition and prediction of germinability could not be determined. Other aspects such as membrane damage, capacity for protein synthesis and seed lot homogeneity in relation to germination are discussed.

Keywords

germinability markergermination capacityin vivo protein synthesismembrane damageproteinseedsunflower
Type
Research Papers
Information
Seed Science Research , Volume 2 , Issue 4 , December 1992 , pp. 223 - 230
Copyright
Copyright © Cambridge University Press 1992

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References

Bewley, J.D., Larsen, K.M. and Papp, J.E.T. (1983) Water stress induced changes in the pattern of protein synthesis in maize seedling mesocotyls: a comparison with the effects of heat shock. Journal of Experimental Botany 34, 1126–1133.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1985) Seeds. Physiology of development and germination. Plenum Publishing Corporation, New York.CrossRefGoogle Scholar
Blowers, L.E., Stormonth, D.A. and Bray, C.M. (1980) Nucleic acid and protein synthesis and loss of vigour in germinating wheat embryos. Planta 150, 19–25.CrossRefGoogle ScholarPubMed
Blowers, L.E., Stormonth, D.A. and Bray, C.M. (1985) Protein synthesis and loss of vigour in germinating wheat embryos. Plant Science Letters 37, 257–264.CrossRefGoogle Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 243–254.CrossRefGoogle ScholarPubMed
Bray, C.M. and Smith, C.A.D. (1985) Stored polyadenylated RNA and loss of vigour in germinating wheat embryos. Plant Science 38, 71–79.CrossRefGoogle Scholar
Brocklehurst, P.A. and Fraser, R.S.S. (1980) Ribosomal RNA integrity and rate of seed germination. Planta 148, 417–421.CrossRefGoogle ScholarPubMed
Cocucci, S. and Cocucci, M. (1977) Effects of ABA, GA3, and FCon the development of potassium uptake in germinating radish seeds. Plant Science Letters 10, 85–95.CrossRefGoogle Scholar
Cocucci, S.M., Ranieri, A. and Zocchi, G. (1986) Potassium-dependent increase in RNA and protein synthesis in the early phase of incubation of the thermodormant Phacelia tanacetifolia seeds. Plant Physiology 80, 891–894.CrossRefGoogle ScholarPubMed
Dalgalarrondo, M., Raymond, J. and Azanza, J.L. (1984) Sunflower seed proteins characterization and subunit composition of the globulin fraction. Journal of Experimental Botany 35, 1613–1628.CrossRefGoogle Scholar
Damerval, C., Le Guilloux, M., Blaisonneau, J. and De Vienne, D. (1987) A simplification of Heukeshoven and Dermick's silver staining of proteins. Electrophoresis 8, 158–159.CrossRefGoogle Scholar
Davies, H.V. and Chapman, J.M. (1979) The control of food mobilization in seeds of Cucumis sativus L. II. The role of the embryonic axis. Planta 146, 585–590.CrossRefGoogle ScholarPubMed
Dell' Aquila, A. (1987) Mean germination time as a monitor of the seed ageing. Plant Physiology and Biochemistry 25, 761–768.Google Scholar
De Meillon, S., Small, J.G.C. and Van de Venter, H.A. (1990) The respiratory metabolism of Strelitzia juncea Ait seeds: the effect of dormancy release through oxygen incubation of the seeds on the activity of glucose-6-phosphatedehydrogenase and 6-phosphogluconate dehydrogenase. Journal of Experimental Botany 41, 709–714.CrossRefGoogle Scholar
Di Nola, L. and Mayer, A.M. (1986) Effect of temperature on glycerol metabolism in membranes and on phospholipases C and D of germinating pea embryos. Phytochemistry 25, 2255–2259.CrossRefGoogle Scholar
Francis, A. and Coolbear, P. (1984) Changes in the membrane phospholipid composition of tomato seeds accompanying loss of germination capacity causedby controlled deterioration. Journal of Experimental Botany 35, 1764–1770.CrossRefGoogle Scholar
Ghosh, B. and Chaudhuri, M.M. (1984) Ribonucleic acid breakdown and loss of protein synthetic capacity with loss of viability of rice embryos (Oryza sativa). Seed Science and Technology 12, 669–677.Google Scholar
Gidrol, X., Noubhani, A., Mocquot, B., Fournier, A. and Pradet, A. (1988) Effect of accelerated aging on protein synthesis in two legume seeds. Plant Physiology and Biochemistry 26, 281–288.Google Scholar
Goffner, D. (1989) Contribution à l'étude dela synthèse des protéines dans la graine de tournesol. Thèse de Doctorat d'Université, Toulouse.Google Scholar
Hailstones, M.D. and Smith, M.T. (1988) Lipid peroxidation in relation to declining vigour in seeds of soya and cabbage. Journal of Plant Physiology 133, 452–456.CrossRefGoogle Scholar
Helm, K.W., Petersen, N.S. and Abernethy, R.H. (1989) Heat shock response of germinating embryos of wheat. Effects of imbibition time and seed vigor. Plant Physiology 90, 598–605.CrossRefGoogle ScholarPubMed
Howarth, C.J. (1990) Heat shock proteins in sorghum and pearl millet; ethanol, sodium arsenite, sodium malonate and the development of thermotolerance. Journal of Experimental Botany 41, 877–883.CrossRefGoogle Scholar
Hurkman, W.J. and Tanaka, C.K. (1988) Polypeptide changes induced by salt stress, water deficit, and osmotic stress in barley roots: a comparison using two-dimensional gel electrophoresis. Electrophoresis 9, 781–787.CrossRefGoogle Scholar
Kermode, A.R. and Bewley, J.D. (1985) The role of maturation drying in the transition from seed development to germination. I. Acquisition of desiccation-tolerance and germinability during development of Ricinus communis L. seeds. Journal of Experimental Botany 36, 1906–1915.CrossRefGoogle Scholar
Kermode, A.R. and Bewley, J.D. (1989) Developing seeds of Ricinus communis L., when detached and maintained in an atmosphere of high relative humidity, switch to a germinative mode without the requirement for complete desiccation. Plant Physiology 90, 702–707.CrossRefGoogle Scholar
Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Lin, C., Roberts, J.K. and Key, J.L. (1984) Acquisition of thermotolerance in soybean seedlings. Synthesis and accumulation of heat shock proteins and their cellular localization. Plant Physiology 74, 152–160.CrossRefGoogle ScholarPubMed
Merrien, A., Quinsac, A. and Maisonneuve, C. (1988) Variabilité de la teneur en protéines des graines de tournesol en relation avec l'état protéique foliaire. pp. 158–169. in Proceedings of the 12th Inte rnational Sunflower Conference, Novi-Sad, Yugoslavia, 25–29 July 1988, Vol. 1.Google Scholar
Meyer, Y., Grosset, J., Chartier, Y. and Cleyet-Marel, J.C. (1988) Preparation by two-dimensional electrophoresis of proteins for antibody production: antibodies against proteins whose synthesis is reduced by auxin in tobacco mesophyll protoplasts. Electrophoresis 9, 704–712.CrossRefGoogle ScholarPubMed
O'Farrell, P.Z., Goodman, H.M. and O'Farrell, P.H. (1977) High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133–1142.CrossRefGoogle ScholarPubMed
Parrish, D.J. and Leopold, A.C. (1978) Confounding of alternate respiration by lipoxygenase activity. Plant Physiology 62, 470–472.CrossRefGoogle ScholarPubMed
Petruzzelli, L., Lioi, L., Carello, G., Morgutti, S. and Cocucci, S. (1982) The effect of fusicoccin and monovalent cations on the viability of wheat seed. Journal of Experimental Botany 33, 118–124.CrossRefGoogle Scholar
Petruzzelli, L. and Taranto, G. (1984) Phospholipid changes in wheat embryos aged under different storage conditions. Journal of Experimental Botany 35, 517–520.CrossRefGoogle Scholar
Priestley, D.A., Werner, B.G. and Leopold, A.C. (1985) The susceptibility of soybean seed lipids to artificially enhanced atmospheric oxidation. Journal of Experimental Botany 36, 1653–1659.CrossRefGoogle Scholar
Pukacka, S. and Kuiper, P.J.C. (1988) Phospholipid compositionand fatty acid peroxidation during ageing of Acer platanoides seeds. Physiologia Plantarum 72, 89–93.CrossRefGoogle Scholar
Schopfer, P. and Plachy, C. (1984) Control of seed germination by abscisic acid. II. Effect on embryo water uptake in Brassica napus L. Plant Physiology 76, 155–160.CrossRefGoogle ScholarPubMed
Shutov, A.D. and Vaintraub, I.A. (1987) Degradation of storage proteins in germinating seeds. Phytochemistry 26, 1557–1566.CrossRefGoogle Scholar
Smith, C.A.D., Rushton, P. and Bray, C.M. (1986) Polyadenylated RNA metabolism and loss of vigour and viability in germinating wheat embryo. Physiologia Plantarum 67, 310–343.CrossRefGoogle Scholar
This, P., Goffner, D., Raynal, M., Chartier, Y. and Delseny, M. (1988) Characterization of major storage proteins of sunflower and their accumulation. Plant Physiology and Biochemistry 26, 125–132.Google Scholar
Thompson, S., Bryant, J.A. and Brocklehurst, P.A. (1987) Changes in levels and integrity of ribosomal RNA during seed maturation and germination in carrot (Daucus carota). Journal of Experimental Botany 38, 1343–1350.CrossRefGoogle Scholar
Welbaum, G.E. and Bradford, K.J. (1988) Water relations of seed development and germination in muskmelon (Cucumis melo L.). I. Water relationsof seed and fruit development. Plant Physiology 86, 406–411.CrossRefGoogle Scholar
Wilson, D.O. and MacDonald, J.R. (1986) The lipid peroxidationmodel of seed ageing. Seed Science and Technology 14, 269–300.Google Scholar