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Soybean seed coat cup unloading on plants with low-raffinose, low-stachyose seeds

Published online by Cambridge University Press:  01 September 2009

Suzanne M. Kosina ,
Alexander Castillo ,
Steven R. Schnebly  and
Ralph L. Obendorf
Suzanne M. Kosina
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University, Ithaca, NY14853, USA
Alexander Castillo
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University, Ithaca, NY14853, USA
Steven R. Schnebly
Affiliation:
Pioneer Hi-Bred, A DuPont Business, 810 Sugar Grove Ave., Hwy44, Dallas Center, IA50063, USA
Ralph L. Obendorf*
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University, Ithaca, NY14853, USA
*
*Correspondence Fax: +1 607 255 2644 Email: rlo1@cornell.edu
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Abstract

Sucrose, raffinose and stachyose accumulate in soybean [Glycine max L. (Merrill)] embryos during seed maturation. To determine the relationship of plant maternal composition on seed composition, soluble carbohydrates in three 1-cm2 leaf punches at three plant growth stages (R2, R3, R6) and in seed coat cup exudates in planta were analysed at four 30-min intervals on soybean plants (R5) with low-raffinose, low-stachyose (LRS) seeds expressing the mutant stc1 phenotype; low-raffinose, low-stachyose and low-phytin (LRSP1, LRSP2) seeds expressing the mutant mips phenotype; or normal raffinose, stachyose and phytin (CHECK) seeds expressing the Stc1 and Mips phenotype. Leaf sucrose (23.6 μg cm− 2), myo-inositol (9.3 μg cm− 2), d-chiro-inositol (6.7 μg cm− 2), d-ononitol (0.76 μg cm− 2), d-pinitol (50.1 μg cm− 2) and total soluble carbohydrates (107.1 μg cm− 2) were not significantly different between phenotypes. d-chiro-Inositol, myo-inositol, d-pinitol and sucrose were unloaded from soybean seed coat cups in planta at decreasing rates over the four sequential periods of sampling. Unloading rates of sucrose and myo-inositol were highest for LRS, d-pinitol was highest for LRSP2, and d-chiro-inositol was not different between LRS, LRSP1, LRSP2 and CHECK. Free cyclitols were 60% of total soluble carbohydrates in leaves and 20% in seed coat cup exudates. Except for sucrose and d-pinitol, seed phenotype had little influence on the composition of compounds unloaded from seed coats to maturing embryos of low-raffinose, low-stachyose seeds. Maternally supplied cyclitols may contribute, in part, to changes in the composition of cyclitol galactosides stored in mature seeds.

Keywords

d-chiro-inositolGlycine max (L.) Merrillmyo-inositold-pinitolseed coat cup unloadingsucrose
Type
Research Article
Information
Seed Science Research , Volume 19 , Issue 3 , September 2009 , pp. 145 - 153
Copyright
Copyright © Cambridge University Press 2009

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