Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-10T16:05:11.149Z Has data issue: false hasContentIssue false
  • English
  • Français

Sugarbeet seed priming: solubilization of the basic subunit of 11-S globulin in individual seeds

Published online by Cambridge University Press:  22 February 2007

Sophie Bourgne ,
Claudette Job  and
Dominique Job
Sophie Bourgne
Affiliation:
Laboratoire Mixte CNRS/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
Claudette Job
Affiliation:
Laboratoire Mixte CNRS/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
Dominique Job*
Affiliation:
Laboratoire Mixte CNRS/Aventis (UMR1932), Aventis CropScience, 14–20 rue Pierre Baizet, 69263, Lyon CEDEX 9, France
*
*Correspondence Tel: +33 4 72 85 21 79 Fax: +33 4 72 85 22 97 Email: dominique.job@aventis.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Priming of sugarbeet (Beta vulgaris L.) seeds induces increased solubilization of the basic B-subunit of 11-S globulin (a major seed storage protein in sugarbeet). Using a sensitive single-seed ELISA, the soluble and total B-subunit contents of individual untreated and primed sugarbeet seeds were measured. With the untreated seeds, there was a 160-fold range of the soluble B-subunit content among individual seeds. The individual primed seeds also exhibited large variations in their soluble B-subunit content, yet only over a five-fold range. Furthermore, the frequency distributions of soluble B-subunit content were markedly different for the primed and untreated seed populations; the primed seed population exhibited a substantially higher median than that for the untreated seed population. In marked contrast, and as expected from results with pooled seed samples, the distributions of total B-subunit content were superimposed when comparing untreated and primed seed populations.

Keywords

Beta vulgarisseed germinationseed primingsingle-seed assay11-S globulin mobilizationstorage proteinssugarbeet
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 2 , June 2000 , pp. 153 - 161
Copyright
Copyright © Cambridge University Press 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bedi, S. and Basra, A.S. (1993) Chilling injury in germinating seeds: basic mechanisms and agricultural implications. Seed Science Research 3, 219–229.CrossRefGoogle Scholar
Bradford, K.J. (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience 21, 1105–1112.CrossRefGoogle Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Chareyre, S., Kersulec, A., Job, D. and Job, C. (1998) The use of an ELISA to quantitate the extent of 11S globulin mobilization in untreated and primed sugar beet seed lots. Comptes Rendus de l'Académie des Sciences Paris Série III 321, 705–711.Google Scholar
Fujikura, Y., Kraak, H.L., Basra, A.S. and Karssen, C.M. (1993) Hydropriming, a simple and inexpensive priming method. Seed Science and Technology 21, 639–642.Google Scholar
Hegarty, T.W. (1978) The physiology of seed hydration and dehydration, and the relation between water stress and the control of germination: a review. Plant, Cell and Environment 1, 101–119.CrossRefGoogle Scholar
Heydecker, W. and Coolbear, P. (1977) Seed treatments for improved performance – survey and attempted prognosis. Seed Science and Technology 5, 353–425.Google Scholar
Heydecker, W., Higgins, J. and Gulliver, R.L. (1973) Accelerated germination by osmotic seed treatment. Nature 246, 42–44.CrossRefGoogle Scholar
Job, C., Kersulec, A., Ravasio, L., Chareyre, S., Pepin, R. and Job, D. (1997) The solubilization of the basic subunit of sugarbeet seed 11-S globulin during priming and early germination. Seed Science Research 7, 225–243.CrossRefGoogle Scholar
Karssen, C.M., Haigh, A., van der Toorn, P. and Weges, R. (1989) Physiological mechanisms involved in seed priming. pp. 269–280in Taylorson, R.B. (Ed) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Lawrence, D.M., Halmer, P. and Bowles, D.J. (1990) Mobilisation of storage reserves during germination and early seedling growth of sugar beet. Physiologia Plantarum 78, 421–429.CrossRefGoogle Scholar
Parera, C.A. and Cantliffe, D.J. (1994) Presowing seed priming. Horticultural Reviews 16, 109–141.Google Scholar
Rowse, H.R. (1996) Drum priming – a non-osmotic method of priming seeds. Seed Science and Technology 24, 281–294.Google Scholar
Shewry, P.R., Napier, J.A. and Tatham, A.S. (1995) Seed storage proteins: structures and biosynthesis. The Plant Cell 7, 945–956.Google ScholarPubMed
Still, D.W. and Bradford, K.J. (1997) Endo-β-mannanase activity from individual tomato endosperm caps and radicle tips in relation to germination rates. Plant Physiology 113, 21–29.CrossRefGoogle ScholarPubMed
Tarquis, A.M. and Bradford, K.J. (1992) Prehydration and priming treatments that advance germination also increase the rate of deterioration of lettuce seeds. Journal of Experimental Botany 43, 307–317.CrossRefGoogle Scholar
Taylor, A.G., Klein, D.E. and Whitlow, T.H. (1988) SMP: solid matrix priming of seeds. Scientia Horticulturae 37, 1–11.CrossRefGoogle Scholar