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Synthesis of extrachromosomal DNA and telomere-related sequences in germinating wheat embryos

Published online by Cambridge University Press:  19 September 2008

M. Bucholc  and
J. Buchowicz
M. Bucholc
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka, 02-532 Warsaw, Poland
J. Buchowicz*
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka, 02-532 Warsaw, Poland
*
* Correspondence
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Abstract

Nuclear DNA of wheat embryos was probed with a synthetic oligonucleotide (C3TA3C3TA3) to assess changes in the length of telomeres during storage and germination. Chromosomal DNA of dry embryos, especially those derived from long-term-stored caryopses, was considerably depleted of telomeric repeats, but, shortly after the start of imbibition, the chromosomal DNA became rich in sequences hybridizing to the telomeric probe. Simultaneously, newly synthesized telomere-related sequences appeared in an extrachromosomal form that could be separated from bulk nuclear DNA by velocity and isopycnic centrifugations. It is concluded that, in addition to typical repair events, rapid synthesis of telomeric repeats occurs in wheat embryos at the onset of germination.

Keywords

extrachromosomal DNAgerminationsenescencetelomereTriticum aestivum L.
Type
Research Papers
Information
Seed Science Research , Volume 2 , Issue 3 , September 1992 , pp. 141 - 146
Copyright
Copyright © Cambridge University Press 1992

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