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Vicia faba germination: Synchronized cell growth and localization of nucleolin and α-tubulin.

Published online by Cambridge University Press:  22 February 2007

Yuzo Fujikura ,
Jaroslav Doležel ,
Jarmira Cíhalíková ,
Laszlo Bögre ,
Erwin Heberle-Bors ,
Heribert Hirt  and
Pavla Binarová
Yuzo Fujikura*
Affiliation:
Institute of Experimental Botany, Norman Borlaug Center for Plant Science De Montfort University, Sokolovska 6, 77200 Olomouc, Czech Republic
Jaroslav Doležel
Affiliation:
Institute of Experimental Botany, Norman Borlaug Center for Plant Science De Montfort University, Sokolovska 6, 77200 Olomouc, Czech Republic
Jarmira Cíhalíková
Affiliation:
Institute of Experimental Botany, Norman Borlaug Center for Plant Science De Montfort University, Sokolovska 6, 77200 Olomouc, Czech Republic
Laszlo Bögre
Affiliation:
Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Dr. Bohrgasse 9, A-1030, Austria
Erwin Heberle-Bors
Affiliation:
Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Dr. Bohrgasse 9, A-1030, Austria
Heribert Hirt
Affiliation:
Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Dr. Bohrgasse 9, A-1030, Austria
Pavla Binarová
Affiliation:
Institute of Experimental Botany, Norman Borlaug Center for Plant Science De Montfort University, Sokolovska 6, 77200 Olomouc, Czech Republic
*
*Correspondence Department of Pathophysiology, 1st Medical Faculty, Charles University, U nemocnice 5, 12853 Prague 2, Czech Republic Tel: +420.2.24.91.49.29 Fax: +420.2.24.91.28.34 Email: fuji@lf1.cuni.cz
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Abstract

The first cell cycle of Vicia faba L. seeds, which begins upon imbibition of dry seeds and is completed at the first mitosis after radicle protrusion, was characterised by the flow cytometry and immunodetection of nucleolin and tubulins in root tip meristems. Flow cytometry revealed highly synchronised profiles from the quiescent G1 phase to the late G2 phase, indicating uniform cell cycle progression within a root tip until the first mitosis. Using immunoblotting, nucleolin was detected in two distinct bands with the apparent molecular masses of 89 and 99 kD; the former was detected only in seeds imbibed at 4°C for 1 day whereas the latter was found at all stages examined, suggesting that the 89 kD nucleolin may be seed-specific. Unusual localization of nucleolin in cold-imbibed seeds, undetectable in half of the cells and present in nucleoplasm, was revealed by immunofluorescence microscopy. While α- and β-tubulin were detected at all stages and no significant changes in accumulation of the proteins were observed, few microtubules were detected at the beginning of germination when cells were still in the G1 phase, suggesting that microtubules may be depolymerized in the dry seeds.

Keywords

Cell cycleflow cytometrygerminationimmunofluorescence microscopymicrotubulesnucleolinVicia faba L.
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 4 , April 1999 , pp. 297 - 304
Copyright
Copyright © Cambridge University Press 1999

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