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Xenia in bahiagrass: gene expression at initial seed formation

Published online by Cambridge University Press:  21 December 2018

Florencia I. Pozzi ,
Guillermo R. Pratta ,
Carlos A. Acuña  and
Silvina A. Felitti
Florencia I. Pozzi
Affiliation:
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA Zavalla, Provincia de Santa Fe, Argentina
Guillermo R. Pratta
Affiliation:
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA Zavalla, Provincia de Santa Fe, Argentina
Carlos A. Acuña
Affiliation:
Instituto de Botánica del Nordeste (IBONE), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sargento Cabral 2131, W3402BKG Corrientes, Argentina
Silvina A. Felitti*
Affiliation:
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA Zavalla, Provincia de Santa Fe, Argentina
*
Author for correspondence: Silvina A. Felitti, Email: felitti@iicar-conicet.gob.ar
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Abstract

Xenia is the direct effect of pollen genotype on the development and characteristics of the seed and fruit in the period that spans from fertilization to seed germination. Xenia effects cause phenotypic variations in the seed and fruit, which have importance for seed and fruit production but are not heritable to the progeny. Two hypotheses have been proposed as a mechanism for xenia: the hormonal hypothesis and the mobile mRNAs hypothesis. Although xenia effects have been studied in seeds and fruits in many crops, its effects and mechanism have been poorly studied at the molecular level. The aim of this work was to perform an initial screening of the xenia effect in the hybrid endosperm at the molecular level by differential gene expression among different pollen genotype sources from Paspalum notatum Flüggé. In order to characterize xenia effects and mechanisms, crosses were made between an emasculated mother plant with donors from two different pollen genotypes. RNA was isolated from ovaries 3 h after pollination. Some of the 24 differentially expressed transcript-derived fragments (DETDFs) provided relevant information. Four of those DETDFs were related to germination, pollen tube growth and pollen–pistil interaction. Seven DETDFs were associated with seed development and production. Finally, four DETDFs were predicted to encode for mobile mRNAs. These DETDFs might be involved in xenia effects and mechanisms in P. notatum.

Keywords

endospermgene expressionmobile mRNAspseudogamyseedxenia
Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 1 , March 2019 , pp. 29 - 37
Copyright
Copyright © Cambridge University Press 2018 

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