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Stringent Programming of DNA Methylation in Humans

Published online by Cambridge University Press:  21 February 2012

Hnin T. Aung ,
Dion K. Harrison ,
Ian Findlay ,
John S. Mattick ,
Nicholas G. Martin  and
Bernard J. Carroll
Hnin T. Aung
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
Dion K. Harrison
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
Ian Findlay
Affiliation:
Australian Genome Research Facility, The University of Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, Australia.
John S. Mattick
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, Australia.
Nicholas G. Martin
Affiliation:
Queensland Institute of Medical Research, Brisbane, Australia.
Bernard J. Carroll*
Affiliation:
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, Australia. b.carroll@uq.edu.au
*
*Address for correspondence: Bernard J. Carroll, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.

Abstract

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We describe a PCR-based method called Amplified Methylation Polymorphism (AMP) for scanning genomes for DNA methylation changes. AMP detects tissue-specific DNA methylation signatures often representing junctions between methylated and unmethylated DNA close to intronexon junctions and/or associated with CpG islands. Identical AMP profiles are detected for healthy, young, monozygotic twins.

Keywords

DNA methylationmethylation-sensitive PCRtissue-specificidentical twins
Type
Articles
Information
Twin Research and Human Genetics , Volume 13 , Issue 5 , 01 October 2010 , pp. 405 - 411
Copyright
Copyright © Cambridge University Press 2010