Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-10T17:47:42.642Z Has data issue: false hasContentIssue false
  • English
  • Français

NADPH-diaphorase neurones of human retinae have a uniform topographical distribution

Published online by Cambridge University Press:  02 June 2009

Jan M. Provis  and
John Mitrofanis
Jan M. Provis
Affiliation:
Department of Anatomy, University of Sydney, Australia Save Sight and Eye Health Institute, University of Sydney, Australia
John Mitrofanis
Affiliation:
Department of Anatomy, University of Sydney, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

We have examined the morphology and distribution of neurones that contain nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase in human retinae. NADPH-diaphorase reactivity was observed in three different classes of amacrine cells (ND1, ND2, ND3 cells) and in the cone photoreceptors. ND1 cells had relatively large somata (mean, 12.3 ¼m) located in the inner nuclear layer (INL) and in the ganglion cell layer (GCL). Their dendrites were often strongly labeled and spread into either the middle or outer strata of the inner plexiform layer (IPL). The somata of ND2 cells were medium-sized (mean, 8.2 ¼m) and located in the INL and in the GCL; their dendrites were usually beaded and often spread in either the middle or outer strata of the IPL. ND3 cells had small, round somata (mean, 5.2 ¼m) located in either the INL or GCL, and were without labeled processes. The total number of NADPH-diaphorase cells (all classes) was estimated at 118,000, with a mean density of about 100/mm2. The most striking fea ture of NADPH-diaphorase cells in humans was that their distribution was relatively uniform across the retina, with no evidence of a peak in density at the foveal rim.

Keywords

Amacrine cellsConesFovea centralisRetinal topography
Type
Short Communication
Information
Visual Neuroscience , Volume 4 , Issue 6 , June 1990 , pp. 619 - 623
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cobcroft, M.D., Vaccaro, T.M. & Mitrofanis, J. (1989). Distinct patterns of distribution among NADPH-diaphorase neurones of the guinea pig retina. Neuroscience Letters 103, 1–7.CrossRefGoogle ScholarPubMed
Halasz, P. & Martin, P. (1984). A microcomputer-based system for semi-automatic analysis of histochemical sections. Royal Microscopic Society Proceedings 19, 312.Google Scholar
Hughes, A. (1971). The topographical relationships between the anatomy and physiology of the rabbit visual system. Documenta Ophthalmologia 30, 33–159.CrossRefGoogle ScholarPubMed
Kolb, H., Nelson, R. & Mariani, A.P. (1981). Amacrine cells, bipolars, and ganglion cells of the cat retina: a golgi study. Vision Research 21, 1081–1114.CrossRefGoogle ScholarPubMed
Kolb, H. & Nelson, R. (1985). Functional neurocircuitry of amacrine cells in the cat retina. In Neurocircuitry of the Retina. A Cajal Memorial, ed. Gallego, A. & Gouras, P., pp. 215–232. Amsterdam: Elsevier.Google Scholar
Mitrofanis, J. (1989). The development of NADPH-diaphorase cells in the rat's retina. Neuroscience Letters 102, 165–172.CrossRefGoogle ScholarPubMed
Mitrofanis, J., Robinson, S.R. & Provis, J.M. (1989). Somatostatinergic neurones of the developing human and cat retinae. Neuroscience Letters 104, 209–216.CrossRefGoogle ScholarPubMed
Mitrofanis, J. & Provis, J.M. (1990). A unique soma size gradient among catecholaminergic neurones of human retinae. Brain Research (in press).CrossRefGoogle Scholar
Müller, F., Wässle, H. & Brecha, N. (1988). NADPH-diaphorase positive amacrine cells show GABA-like immunoreactivity in cat retina. European Journal of Neuroscience (Suppl.) 1, 153.Google Scholar
Osterberg, G. (1935). Topography of the layer of rods and cones in the human retina. Acta Opthalmology (Suppl.) Kbh, 6.Google Scholar
Provis, J.M., van, Driel D., Billson, F.A. & Russell, P. (1985). Development of the human retina: patterns of cell distribution and redistribution in the ganglion cell layer. Journal of Comparative Neurology 233, 429–451.CrossRefGoogle ScholarPubMed
Rapaport, D.H. & Stone, J. (1983). Time course of morphological differentiation of cat retinal ganglion cells. Journal of Comparative Neurology 221, 42–52.CrossRefGoogle ScholarPubMed
Sagar, S.M. (1986). NADPH-diaphorase histochemistry in the rabbit retina. Brain Research 373, 153–158.Google Scholar
Sagar, S.M. & Marshall, P.E. (1988). Somatostatin-like immunoreactive material in associational ganglion cells of human retina. Neuroscience 27, 507–516.CrossRefGoogle ScholarPubMed
Sandell, J.H. (1985). NADPH-diaphorase cells in the mammalian inner retina. Journal of Comparative Neurology 238, 466–472.CrossRefGoogle ScholarPubMed
Stone, J. & Johnston, E. (1981). The topography of primate retina: a study of the human, bushbaby, and New- and Old-World monkeys. Journal of Comparative Neurology 196, 205–223.CrossRefGoogle ScholarPubMed
Straznicky, C. & Hiscock, J. (1989). Neuropeptide Y immunoreactivity in neurones of the human retina. Vision Research 29, 1041–1048.Google Scholar
Vaccaro, T.M., Cobcroft, M.D., Provis, J.M. & Mitrofanis, J. (1990). NADPH-diaphorase reactivity in adult and developing cat retinae. Cell and Tissue Research (submitted).Google Scholar
Vaney, D.I. & Young, H.M. (1988). GABA-like immunoreactivity in NADPH-diaphorase amacrine cells of the rabbit retina. Brain Research 474, 380–385.CrossRefGoogle ScholarPubMed
Wässle, H., Hoon, Chun M. & Müller, F. (1987). Amacrine cells in the ganglion cell layer of the cat retina. Journal of Comparative Neurology 265, 391–408.CrossRefGoogle ScholarPubMed
Young, H.M. & Vaney, D.I. (1989). GABA-like immunoreactivity in NADPH-diaphorase amacrine cells of rabbit and rat retinae. Neuroscience Letters (Suppl.) 27, S114.Google Scholar