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Organization and development of horizontal cells in the goldfish retina, II: Use of monoclonal antibody MH1

Published online by Cambridge University Press:  02 June 2009

You-Wei Peng  and
Dominic Man-Kit Lam
You-Wei Peng
Affiliation:
Alice R. McPherson Laboratory of Retina Research, the Center for Biotechnology, Baylor College of Medicine The Woodlands Texas
Dominic Man-Kit Lam
Affiliation:
Alice R. McPherson Laboratory of Retina Research, the Center for Biotechnology, Baylor College of Medicine The Woodlands Texas
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Abstract

We have produced and characterized a monoclonal antibody, MH1, which selectively labels rod horizontal cells and Miiller cells in the goldfish retina. Biochemical and tissue distribution studies indicate that MH1 may recognize four out of five classes of intermediate filament proteins in goldfish: vimentin, desmin, glial fibrillary acidic protein (GFAP), and keratin, but not neurofilament. The intermediate filament which is labeled strongest in the retina is vimentin. In the goldfish retina, the only type of horizontal cells recognized by MH1 appear to be rod horizontal cells. This result suggests that the rod horizontal cell, an interneuron, and Miiller (glial) cells share a common antigen: vimentin, which is usually only expressed in mesenchymal origin cells.

The development of rod horizontal cells in the goldfish retina was also studied using MH1. The cells were not labeled by MH1 until 4–6 weeks posthatching, a stage in which the animals are already visually active. MH1 also did not label any horizontal cell in the region close to the ora terminalis in the goldfish retina. These results suggest that either the emergence and maturation of rod horizontal cells occur late during goldfish retinal development or the expression of vimentin itself occurs late in the development of rod horizontal cells.

Keywords

Horizontal cellsMüller cellsRetinal organization and developmentGoldfishCell-type specific monoclonal antibody
Type
Research Articles
Information
Visual Neuroscience , Volume 8 , Issue 3 , March 1992 , pp. 231 - 241
Copyright
Copyright © Cambridge University Press 1992

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