Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-11T05:04:33.707Z Has data issue: false hasContentIssue false
  • English
  • Français

Organization and development of horizontal cells in the goldfish retina, II: Use of monoclonal antibody MH1

Published online by Cambridge University Press:  02 June 2009

You-Wei Peng  and
Dominic Man-Kit Lam
You-Wei Peng
Affiliation:
Alice R. McPherson Laboratory of Retina Research, the Center for Biotechnology, Baylor College of Medicine The Woodlands Texas
Dominic Man-Kit Lam
Affiliation:
Alice R. McPherson Laboratory of Retina Research, the Center for Biotechnology, Baylor College of Medicine The Woodlands Texas
Get access Rights & Permissions [Opens in a new window]

Abstract

We have produced and characterized a monoclonal antibody, MH1, which selectively labels rod horizontal cells and Miiller cells in the goldfish retina. Biochemical and tissue distribution studies indicate that MH1 may recognize four out of five classes of intermediate filament proteins in goldfish: vimentin, desmin, glial fibrillary acidic protein (GFAP), and keratin, but not neurofilament. The intermediate filament which is labeled strongest in the retina is vimentin. In the goldfish retina, the only type of horizontal cells recognized by MH1 appear to be rod horizontal cells. This result suggests that the rod horizontal cell, an interneuron, and Miiller (glial) cells share a common antigen: vimentin, which is usually only expressed in mesenchymal origin cells.

The development of rod horizontal cells in the goldfish retina was also studied using MH1. The cells were not labeled by MH1 until 4–6 weeks posthatching, a stage in which the animals are already visually active. MH1 also did not label any horizontal cell in the region close to the ora terminalis in the goldfish retina. These results suggest that either the emergence and maturation of rod horizontal cells occur late during goldfish retinal development or the expression of vimentin itself occurs late in the development of rod horizontal cells.

Keywords

Horizontal cellsMüller cellsRetinal organization and developmentGoldfishCell-type specific monoclonal antibody
Type
Research Articles
Information
Visual Neuroscience , Volume 8 , Issue 3 , March 1992 , pp. 231 - 241
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ayoub, G.S. & Lam, D.M.K. (1984). The release of γ-amino butyric acid from horizontal cells of the goldfish (Carassium auratus) retina. Journal of Physiology 55, 191–214.Google Scholar
DrÄGer, U.C. (1983). Coexistence of neurofilaments and vimentin in a neurone of adult mouse retina. Nature 303, 169.CrossRefGoogle Scholar
Dräger, U.C, Edwards, D.L. & Barnstable, C.J. (1984). Antibodies against filamentous components in discrete cell types of the mouse retina. Journal of Neuroscience 4, 2025–2042.CrossRefGoogle ScholarPubMed
Gallego, A. (1982). Organization of the outer plexiform layer of the tetrapod retina: Horizontal cells of mammalian and avian retina. In The Structure of the Eye, ed Hollyfield, J.G., pp. 151–164. New York: Elsevier.Google Scholar
Goldstein, M.E., Sternberger, L.A. & Sternberger, N.H. (1983). Microheterogeneity (”neurotypy”) of neurofilament proteins. Proceedings of the National Academy of Science of the U.S.A. 80, 3103–3105.Google Scholar
Gown, A.M. & Vogel, A.M. (1982). Monoclonal antibodies to intermediate filament proteins of human cells: Unique and cross-reacting antibodies. Journal of Cell Biology 95, 414–424.CrossRefGoogle ScholarPubMed
Johns, P.R. (1977). Growth of the adult goldfish eye. III. Source of the new retinal cells. Journal of Comparative Neurology 176, 343–358.Google Scholar
Johns, P.R. (1982). Formation of photoreceptors in the growing retinas of larval and adult goldfish. Journal of Neuroscience 2, 179–198.CrossRefGoogle Scholar
Johns, P.R. & Easter, S.S. (1977). Growth of the adult goldfish eye—increase in retinal cell number. Journal of Comparative Neurology 176, 331–342.CrossRefGoogle Scholar
Julien, J.P. & Mushynski, W.E. (1983). The distribution of phosphorylation sites among identified proteolytic fragments of mammalian neurofilaments. Journal of Biological Chemistry 258, 4109–4125.CrossRefGoogle ScholarPubMed
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680–685.CrossRefGoogle ScholarPubMed
Lam, D.M.K. (1975). Biosynthesis of gamma-amino butyric acid by isolated axons of cone horizontal cells in the goldfish retina. Nature 254, 345–347.CrossRefGoogle Scholar
Lam, D.M.K. (1976). Synaptic chemistry of identified cells in the vertebrate retina. Cold Spring Harbor Symposia on Quantitative Biology XL, 571–579.Google Scholar
Lee, V., Wu, H.L. & Schlaepfer, W.W. (1982). Monoclonal antibodies recognize individual neurofilament triplet proteins. Proceedings of the National Academy of Science of the U.S.A. 79, 6089–6092.Google Scholar
Lee, V., Page, C., Wu, H.L. & Schlaepfer, W.W. (1982). Monoclonal antibodies to gel-excised glial filament protein and their reactivities with other intermediate filament proteins. Journal of Neurochemistry 42, 25–32.CrossRefGoogle Scholar
Lee, V., Carden, M.J. & Trojanowski, J.Q. (1986). Novel monoclonal antibodies provide evidence for the in situ existence of a nonphosphorylated form of the largest neurofilament subunit. Journal of Neuroscience 6, 850–858.CrossRefGoogle ScholarPubMed
Lowry, O., Raseburough, K.O., Farr, A. & Ranall, R. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Marc, R.E. (1986). The development of retinal networks. In The Retina: A Model for Cell Biology Studies, Part I, ed. Adler, R. & Farber, D., pp. 17–65.Google Scholar
Marc, R.E., Stell, W.K., Bok, D. & Lam, D.M.K. (1978). GABAergic pathways in the goldfish retina. Journal of Comparative Neurology 182, 221–246.Google Scholar
Naka, K.I. (1977). Functional organization of catfish retina. Journal of Neurophysiology 40, 26–43.Google Scholar
O'Farrell, P.H. (1975). High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–4021.Google Scholar
Peng, Y.W. & Lam, D.M.K. (1991). Organization and development of horizontal cells in the goldfish retina, I: The use of monoclonal antibody AT101. Visual Neuroscience 6, 357–370.CrossRefGoogle ScholarPubMed
Pruss, R.M., Mirsky, R., Raff, M.C., Thorpe, R., Dowding, L. & Anderton, B.H. (1981). All classes of intermediate filaments share a common antigen determinant defined by a monoclonal antibody. Cell 27, 419–428.CrossRefGoogle Scholar
Raymond, P.A. (1985). Cytodifferentiation of photoreceptors in larval goldfish: delayed maturation of rods. Journal of Comparative Neurology 236, 90–105.Google Scholar
Raymond, P.A. & Rivlin, P.K. (1987). Germinal cells in the goldfish retina that produce rod photoreceptors. Developmental Biology 122, 120–138.CrossRefGoogle ScholarPubMed
Raymond, P.A. (1991). In Development of the Visual System, ed Lam, D.M.K. & Shatz, C.J., pp. 59–78.Google Scholar
Sharma, S.C. & Ungar, F. (1980). Histogenesis of the goldfish retina. Journal of Comparative Neurology 191, 373–382.CrossRefGoogle ScholarPubMed
Shaw, G. & Weber, K. (1983). The structure and development of the rat retina: an immunofluorescence microscopical study using anti-bodies specific for intermediate filament proteins. European Journal of Cell Biology 30, 219.Google Scholar
Stell, W.K. & Lightfoot, D.O. (1975). Color-specific interconnections of cones and horizontal cells in the retina of the goldfish. Journal of Comparative Neurology 159, 473–502.CrossRefGoogle ScholarPubMed
Sternberger, L.A. & Sternberger, N.H. (1983). Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ. Proceedings of the National Academy of Science of the U.S.A. 80, 6126–6130.CrossRefGoogle ScholarPubMed
Towbin, H., Stalhelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the U.S.A. 76, 4350–4354.CrossRefGoogle ScholarPubMed
Trojanowski, J.Q., Obrocka, M.A. & Lee, V. (1985). Distribution of neurofilament subunits in neurons and neural processes: Immunohistochemical studies of bovine cerebellum with subunit-specific monoclonal antibodies. Journal of Histochemical Cytochemistry 33, 557–563.Google Scholar
Wray, W., Baulibas, J., Wrap, V.P. & Hancock, R. (1981). Silver staining of proteins in polyacrylamide gels. Analytical Biochemistry 118, 197–203.CrossRefGoogle ScholarPubMed