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Perfusion system components release agents that distort functional properties of rod cyclic nucleotide-gated ion channels

Published online by Cambridge University Press:  01 November 1998

JENNIFER I. CRARY ,
SHARONA E. GORDON  and
ANITA L. ZIMMERMAN
JENNIFER I. CRARY
Affiliation:
Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence
SHARONA E. GORDON
Affiliation:
Department of Physiology, University of Texas-Southwestern Medical Center, Dallas
ANITA L. ZIMMERMAN
Affiliation:
Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence
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Abstract

In switching from studying native cyclic nucleotide-gated (CNG) ion channels in rod cells to studying the corresponding cloned channels expressed in Xenopus oocytes, we changed our perfusion system to a more efficient one. This change involved replacing culture flasks and a small plexiglass/glass chamber with plastic syringes, metal needles, and plastic petri dishes. We now report that these new perfusion system components release agents that distort or obscure measured functional properties of rod CNG channels. The magnitude and time course of appearance of the artifacts vary widely among individual components (e.g. from syringe to syringe). The effects most resemble voltage-dependent block of the channels, giving a decrease in current at positive potentials, and producing distortions of the kinetics and voltage dependence of channel activation.

Keywords

PhotoreceptorsRodsCyclic GMPCNG ion channelsChannel block
Type
SHORT COMMUNICATIONS
Information
Visual Neuroscience , Volume 15 , Issue 6 , November 1998 , pp. 1189 - 1193
Copyright
1998 Cambridge University Press

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