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Characterization of inhibitory postsynaptic currents in rod bipolar cells of the mouse retina

Published online by Cambridge University Press:  01 July 2004

MORITZ J. FRECH  and
KURT H. BACKUS
MORITZ J. FRECH
Affiliation:
Max-Planck-Institut für Hirnforschung, Neuroanatomical Department, Frankfurt am Main, Germany Institute of Physiology II, Cellular Neurophysiology, University of Frankfurt, Frankfurt, Germany
KURT H. BACKUS
Affiliation:
Max-Planck-Institut für Hirnforschung, Neuroanatomical Department, Frankfurt am Main, Germany Institute of Physiology II, Cellular Neurophysiology, University of Frankfurt, Frankfurt, Germany
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Abstract

The synaptic terminals of mammalian rod bipolar cells are the targets of multiple presynaptic inhibitory inputs arriving from glycinergic and GABAergic amacrine cells. To investigate the contribution of these different inhibitory receptor types, we have applied the patch-clamp technique in acutely isolated slices of the adult mouse retina. By using the whole-cell configuration, we measured and analyzed the spontaneous postsynaptic currents (PSCs) in rod bipolar cells. The spontaneous synaptic activity of rod bipolar cells was very low. However, when amacrine cells were depolarized by AMPA or kainate, the PSC frequency in rod bipolar cells increased significantly. These PSCs comprised several types that could be distinguished by pharmacological and kinetic criteria. Strychnine-sensitive, glycinergic PSCs were characterized by a mean peak amplitude of −43.5 pA and a weighted decay time constant (τw) of 10.9 ms. PSCs that persisted in the presence of strychnine, but were completely inhibited by bicuculline, were mediated by GABAARs. They had a mean peak amplitude of −20.0 pA and a significantly faster τw of 5.8 ms. Few PSCs remained in the presence of strychnine and bicuculline, suggesting that they were mediated by GABACRs. These PSCs were characterized by much smaller amplitudes (−6.2 pA) and a significantly slower decay kinetics (τw = 51.0 ms). We conclude that rod bipolar cells express at least three types of functionally different inhibitory receptors, namely GABAARs, GABACRs, and GlyRs that may ultimately regulate the Ca2+ influx into rod bipolar cell terminals, thereby modulating their glutamate release.

Keywords

Patch-clampPostsynaptic currentsGABAA receptorGABAC receptorGlycine receptor
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 4 , July 2004 , pp. 645 - 652
Copyright
2004 Cambridge University Press

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