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Contributions of AMPA- and kainate-sensitive receptors to the photopic electroretinogram of the Xenopus retina

Published online by Cambridge University Press:  04 May 2001

T. SZIKRA  and
P. WITKOVSKY
T. SZIKRA
Affiliation:
MTA-ELTE Neurobiology Group, Department of Neurobiology and Physiology, Eotvos Lorand University, Budapest, Hungary Department of Ophthalmology, New York University School of Medicine, New York
P. WITKOVSKY
Affiliation:
Department of Ophthalmology, New York University School of Medicine, New York Department of Physiology and Neuroscience, New York University School of Medicine, New York
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Abstract

The effects of kainate receptor-preferring glutamate ligands were tested on the electroretinogram (ERG) of the Xenopus retina. Kainate, domoic acid, and 5-iodowillardiine (20–100 μM) acted similarly in every respect. They increased peak amplitudes of the ERG a-, b-, and d-waves significantly over controls. The AMPA-specific antagonist, GYKI 53655, prevented a kainate-induced increase in ERG a- and d-waves, but was without effect on an increase in the b-wave. Once the effect of agonist on the b-wave had peaked, the ERG began to subside, leading to its nearly complete disappearance within 20 min. Prior exposure to GYKI followed by a combination of GYKI + agonist did not significantly slow the rate of b-wave disappearance. Our results indicate that (1) AMPA receptors contribute to ERG a- and d-waves. (2) The kainate-evoked increase in ERG a-, b-, and d-waves probably results, in part, from an excitotoxic swelling of inner retinal processes. (3) The inner retina has a population of GYKI-resistant, kainate-sensitive receptors which may contribute to b-wave generation.

Keywords

KainateAMPAElectroretinogramXenopusExcitotoxicity
Type
Research Article
Information
Visual Neuroscience , Volume 18 , Issue 2 , March 2001 , pp. 187 - 196
Copyright
2001 Cambridge University Press

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