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Light-modulated subcellular localization of the alpha-subunit of GTP-binding protein Gq in crayfish photoreceptors

Published online by Cambridge University Press:  02 June 2009

Akihisa Terakita ,
Hideki Takahama ,
Satoshi Tamotsu ,
Tatsuo Suzuki ,
Takahiko Hariyama  and
Yasuo Tsukahara
Akihisa Terakita
Affiliation:
Institute of Biology, Oita University, Oita 870-11, Japan
Hideki Takahama
Affiliation:
Institute of Biology, Oita University, Oita 870-11, Japan
Satoshi Tamotsu
Affiliation:
First Department of Physiology, Hamamatsu University School of Medicine, Hamamatsu 431-31, Japan
Tatsuo Suzuki
Affiliation:
Department of Pharmacology, Hyogo College of Medicine, Nishinomiya 663, Japan
Takahiko Hariyama
Affiliation:
Graduate School of Information Sciences, SKK Building, Tohoku University, Sendai 980-77, Japan
Yasuo Tsukahara
Affiliation:
Graduate School of Information Sciences, SKK Building, Tohoku University, Sendai 980-77, Japan Photodynamics Research Center, The Institute of Chemical and Physical Research (RIKEN), Aoba-ku Koeji, Sendai 980, Japan
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Abstract

Gq-type GTP-binding protein (Gq) plays an important role in invertebrate visual phototransduction. The subcellular localization of the alpha subunit of visual Gq in crayfish photoreceptor was investigated immunocytochemically and biochemically to demonstrate the details of the rhodopsin-Gq interaction. The localization of Gq(alpha) changed depending on the light condition. In the dark, Gq(alpha) was localized in the whole rhabdoms as the membrane-bound form. In the light, half of the Gq(alpha) was localized in the cytoplasm as the soluble form. The translocation of Gq(alpha) was reversible. The light-modulated translocation possibly controls the amount of Gq that can be activated by rhodopsin. In vitro hydroxylamine treatment of rhabdomeric membranes suggested that the translocation was regulated by the fatty-acid modification of Gq(alpha).

Keywords

Rhabdomeric photoreceptorGq type G-proteinSubcellular localizationPhototransductionPalmitoylation
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 3 , May 1996 , pp. 539 - 547
Copyright
Copyright © Cambridge University Press 1996

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