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Microtubule-associated protein 1A (MAP 1A) is a ganglion cell marker in adult rat retina

Published online by Cambridge University Press:  02 June 2009

Lisa McKerracher ,
Richard B. Vallee  and
Albert J. Aguayo
Lisa McKerracher
Affiliation:
Neurosciences Unit, Montreal General Hospital and McGill University, Montreal, Quebec, Canada
Richard B. Vallee
Affiliation:
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts
Albert J. Aguayo
Affiliation:
Neurosciences Unit, Montreal General Hospital and McGill University, Montreal, Quebec, Canada
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Abstract

We have used antibodies raised against a cytoskeletal protein, microtubule-associated protein 1A (MAP 1A), to stain adult rat retina. In cryostat and polyethylene glycol-embedded radial sections, the fiber layer, ganglion cell layer, and inner plexiform layer were highly immunoreactive, a finding that suggested that the ganglion cell somata, axons, and dendrites were recognized by these antibodies. Retrograde labeling of retinal cell somata from the superior colliculus and dorso-lateral geniculate nucleus to identify ganglion cells showed colocalization of the tracer and immunoreactive cells. Double labeling with nuclear stains revealed that many cells in the ganglion cell layer, which are likely displaced amacrine cells, were not recognized by these antibodies. Furthermore, transection of ganglion cell axons, a procedure that causes retrograde degeneration of many of the axotomized ganglion cells, led to a decrease in the number of anti-MAP 1A immunoreactive cells in retinal wholemounts. Thus, MAP 1A antibodies preferentially stain ganglion cell somata and dendrites but not amacrine cells. These antibodies should be useful ganglion cell markers.

Keywords

Microtubule-associated proteinGanglion cell markerCytoskeletonRetina
Type
Research Article
Information
Visual Neuroscience , Volume 2 , Issue 4 , April 1989 , pp. 349 - 356
Copyright
Copyright © Cambridge University Press 1989

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