Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-10T12:20:28.959Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphology and distribution of serotonin-like immunoreactive amacrine cells in the retina of Bufo marinus

Published online by Cambridge University Press:  02 June 2009

Baosong Zhu  and
Charles Straznicky
Baosong Zhu
Affiliation:
Department of Anatomy and Histology, School of Medicine, The Flinders University of South Australia, Bedford Park, Australia
Charles Straznicky
Affiliation:
Department of Anatomy and Histology, School of Medicine, The Flinders University of South Australia, Bedford Park, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

Using an antibody against serotonin (5-hydroxytryptamine, 5-HT), serotonin-like immunoreactive (serotonin IR) neurons were demonstrated in the retina of adult Bufo marinus. All immunoreactive neurons were identified as amacrine cells (ACs). The dendrites of serotonin-IR ACs branched diffusely and densely throughout all levels of the inner plexiform layer (IPL) of the retina. The great majority of these cell somata were located in the vitread part of the inner nuclear layer (INL) and a few of them (ranging from 9–29 cells) were displaced into the ganglion cell layer (GCL). On the basis of the soma sizes, two populations of serotonin-IR ACs, large (type A) and small (type B), were distinguished. 6-Hydroxydopamine (6-OHDA) injected into the eye abolished immunoreactivity in the recently reported tyrosine hydroxylase (TH)-IR ACs (Zhu & Straznicky, 1990), whereas serotonin-IR ACs remained unaffected.

The number of serotonin-IR cells per retina ranged from 23,750–27,390, with a ratio of 1:1.6 to 1:1.9 between type A and B cells. Both cell types were distributed nonuniformly across the retina. Cell densities were slightly lower in the peripheral (96 cells/mm2) than in the central (164 cells/mm2) retina. Linear regression analysis confirmed the presence of a decreasing density gradient from the retinal center to the retinal margin for both small and large cell types. The analysis of the nearest neighbor distances showed that the retinal distribution of serotonin-IR ACs was orderly.

These results have been taken to indicate that 5-HT-IR cells correspond to a population of serotonincontaining ACs. It is suggested that dopamine and serotonin are contained in two different populations of ACs in the rtina of Bufo marinus.

Keywords

Serotonin (5-hydroxytryptamine, 5-HT)ImmunohistochemistryAmacrine cellsRetinaBufo marinus
Type
Research Article
Information
Visual Neuroscience , Volume 5 , Issue 4 , October 1990 , pp. 371 - 378
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Costa, M., Furness, J.B., Cuello, A.C., Verhofstad, A.A.J., Steinbush, H.W.J. & Elde, R.P. (1982). Neurons with 5-hydroxytrypta- mine-like immunoreactivity in the enteric nervous system: their visualization and reactions to drug treatment. Neuroscience 7, 351–363.CrossRefGoogle Scholar
Ehinger, B. & Florén, I. (1976). Indoleamine-accumulating neurons in the retina of rabbit, cat, and goldfish. Cell and Tissue Research 197, 37–48.Google Scholar
Engbretson, G.A. & Battelle, B.-A. (1987). Serotonin and dopamine in the retina of a lizard. Journal of Comparative Neurology 257, 140–147.CrossRefGoogle ScholarPubMed
Frederick, J.M., Rayborn, M.E. & Hollyfield, J.G. (1989). Serotoninergic neurons in the retina of Xenopus laevis: selective staining, identification, development, and content. Journal of Comparative Neurology 281, 516–531.CrossRefGoogle ScholarPubMed
Gläsener, G., Schmidt, C. & Himstedt, W. (1988). Two populations of serotonin-immunoreactive neurons in the frog (Rana esculenta) retina. Neuroscience Letters 84, 251–254.CrossRefGoogle ScholarPubMed
Halász, P. & Martin, P. (1984). A microcomputer based system for semiautomatic analysis of histochemical sections. Royal Microscopical Society Proceedings 19, 312.Google Scholar
Hiscock, J. & Straznicky, C. (1989 a). Morphological characterization of substance P-like immunoreactive amacrine cells in the anuran retina. Vision Research 29, 293–301.CrossRefGoogle ScholarPubMed
Hiscock, J. & Straznicky, C. (1989 b). Neuropeptide Y-like immunoreactive amacrine cells in the retina of Bufo marinus. Brain Research 494, 55–64.CrossRefGoogle ScholarPubMed
Matthews, D.E. & Farewell, V.T. (1985). Using and understanding medical statistics. Basel; New York: Karger, pp. 123–138.Google Scholar
Nguyen, V.S. & Straznicky, C. (1989). The development and the topographic organization of the retinal ganglion cell layer in Bufo marinus. Experimental Brain Research 75, 345–353.CrossRefGoogle ScholarPubMed
Osborne, N.N., Nesselhut, T., Nicholas, D.A., Patel, S. & Cuello, C. (1982). Serotonin-containing neurons in the vertebrate retinas. Journal of Neurochemistry 39, 1519–1528.CrossRefGoogle ScholarPubMed
Redburn, D.A. (1985). Serotonin transmitter systems in the vertebrate retina. In Retinal Transmitters and Modulators: Models for the Brain, Vol. 2, ed. Morgan, R.A., pp. 107–122. Boca Raton: CRC Press.Google Scholar
Redburn, D.A. & Churchill, L. (1987). An indoleamine system in photoreceptor cell terminals of the Long-Evans rat retina. Journal of Neuroscience 7, 319–329.CrossRefGoogle ScholarPubMed
Reh, T.A. (1986). Regulation of tyrosine hydroxylase-containing amacrine cell number in larval frog retina. Developmental Biology 114, 463–469.CrossRefGoogle ScholarPubMed
Sandell, J.H. & Masland, R.H. (1986). A system of indoleamine-accumulating neurons in the rabbit retina. Journal of Neuroscience 6, 3331–3347.CrossRefGoogle ScholarPubMed
Schütte, M. & Weiler, R. (1987). Morphometric analysis of serotoninergic bipolar cells in the retina and its implications for retinal image processing. Journal of Comparative Neurology 260, 619–626.Google Scholar
Vaney, D. (1986). Morphological identification of serotonin-accumulating neurons in the living retina. Science 233, 444–446.CrossRefGoogle ScholarPubMed
Wässle, H. & Riemann, H.J. (1978). The mosaic of nerve cells in the mammalian retina. Proceedings of the Royal Society B (London) 200, 441–461.Google ScholarPubMed
Wässle, H., Voigt, T. & Patel, B. (1987). Morphological and immunocytochemical identification of indoleamine-accumulating neurons in the cat retina. Journal of Neuroscience 7, 1574–1585.CrossRefGoogle ScholarPubMed
Weiler, R. & Schütte, M. (1985). Morphological and pharmacological analysis of putative serotonergic bipolar and amacrine cells in the retina of a turtle, Pseudomys scripta elegans. Cell and Tissue Research 241, 373–382.CrossRefGoogle Scholar
Witkovsky, P., Eldred, W. & Karten, H.J. (1984). Catecholamineand indolamine-containing neurons in the turtle retina. Journal of Comparative Neurology 228, 217–225.CrossRefGoogle Scholar
Witkovsky, P., Alones, V. & Piccolino, M. (1987). Morphological changes induced in turtle retinal neurons by exposure to 6-hydroxy- dopamine and 5,6-dihydroxytryptamine. Journal of Neurocytology 16, 55–67.CrossRefGoogle Scholar
Yang, S.-Z., Lam, D.M.-K. & Watts, C.B. (1989). Localization of serotonin-like immunoreactive amacrine cells in the larval tiger salamander retina. Journal of Comparative Neurology 287, 28–37.CrossRefGoogle Scholar
Zhu, B.-S. & Straznicky, C. (1990). Dendritic morphology and retinal distribution of tyrosine hydroxylase-like immunoreactive amacrine cells in Bufo marinus. Anatomy and Embryology 181, 365–371.CrossRefGoogle ScholarPubMed
Zhu, B.-S., Hiscock, J. & Straznicky, C. (1990). The changing distribution of neurons in the inner nuclear layer from metamorphosis to adult: a morphometric analysis of the anuran retina. Anatomy and Embryology 181, 585–594.CrossRefGoogle ScholarPubMed