Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-14T09:34:31.293Z Has data issue: false hasContentIssue false
  • English
  • Français

Multifocal electroretinogram in trichromat and dichromat observers under cone isolating conditions

Published online by Cambridge University Press:  05 April 2005

ANNE KURTENBACH ,
JUDITH HEINE  and
HERBERT JÄGLE
ANNE KURTENBACH
Affiliation:
Department of Pathophysiology of Vision and Neuro-ophthalmology, University Eye Hospital, Tuebingen, Germany
JUDITH HEINE
Affiliation:
Department of Pathophysiology of Vision and Neuro-ophthalmology, University Eye Hospital, Tuebingen, Germany
HERBERT JÄGLE
Affiliation:
Department of Pathophysiology of Vision and Neuro-ophthalmology, University Eye Hospital, Tuebingen, Germany
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of this study was to obtain information about single cone class driven activity in the inner and outer retina in humans. We examined outer retinal activity with the multifocal electroretinogram (mfERG) and inner retinal activity using multifocal oscillatory potentials (mfOPs). A standard (black-white) stimulus was used, as well as stimuli aimed at isolating a single photoreceptor class. The results of 10 trichromats were compared to those of 2 protanopes and 2 deuteranopes. At both retinal layers we find that trichromats show cone isolating response amplitudes that reflect the expected number of cones and that single- gene dichromats have a similar total number of functioning cones as trichromats. The ratio of the responses of the L- and M-cones is slightly smaller for the mfOPs than for the mfERGs. The results indicate that there are major changes in the gain of retinal signals after the inner plexiform layer.

Keywords

mfERGmfOPsdichromacycone-isolationretina
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 3 , May 2004 , pp. 249 - 255
Copyright
© 2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albrecht, J., Jägle, H., Hood, D., & Sharpe, L.T. (2002). The multifocal electroretinogram (mfERG) and cone isolating stimuli: Variation in L- and M-cone driven signals across the retina. Journal of Vision 2, 543–558.Google Scholar
Asenjo, A.B., Rim, J., & Oprian, D.D. (1994). Molecular determinants of human red/green color discrimination. Neuron 12, 1131–138.CrossRefGoogle Scholar
Brindley, G.S. (1956). Responses to illumination recorded by microelectrodes from the frog's retina. Journal of Physiology 134, 360–384.CrossRefGoogle Scholar
Brown, K.T. (1968). The electroretinogram: Its components and their origin. Vision Research 8, 633–677.CrossRefGoogle Scholar
de Vries, H. (1946). The heredity of the relative numbers of red and green receptors in the human eye. Genetica 24, 199–212.Google Scholar
Estévez, O. & Spekreijse, H. (1982). The “silent substitution” method in visual research. Vision Research 22, 681–691.CrossRefGoogle Scholar
Hagstrom, S.A., Neitz, J., & Neitz, M. (1998). Variation in cone populations for red–green color vision examined by analysis of mRNA. NeuroReport 9, 1963–1967.CrossRefGoogle Scholar
Hood, D.C., Seiple, W., & Holopigiam, K. (1997). A comparison fo the components of the multifocal and and the full-field ERGs. Visual Neuroscience 14, 533–544.CrossRefGoogle Scholar
Hood, D.C., Frishman, L.J., Saszik, S., & Viswanathan, S. (2002a). Retinal origins of the primate multifocal ERG: Implication for the human response. Investigative Ophthalmology & Visual Science 43, 1673–1685.Google Scholar
Hood, D.C., Yu, A.L., Zhang, X., Albrecht, J., Jägle, H., & Sharpe, L.T. (2002b). The multifocal visual evoked potential and cone-isolating stimuli: Implications for L- to M-cone ratios and normalization. Journal of Vision 2, 178–189.Google Scholar
Jacobs, G.H., & Neitz, J. (1991). Electrophysiological estimates of individual variation in the L/M cone ratio. In Color Vision Deficiencies XI, ed. Drum, B., pp. 107–112. Dordrecht, Kluwer Academic Publishers.
Kremers, J., Usui, T., Scholl, H.P., & Sharpe, L.T. (1999). Cone signal contributions to electroretinograms in dichromats and trichromats. Investigative Ophthalmology & Visual Science 40, 920–930.Google Scholar
Kurtenbach, A., Langrova, H., & Zrenner, E. (2000). Multifocal oscillatory potentials in Type 1 diabetics without retinopathy. Investigative Ophthalmology and Visual Science 41, 3243–3241.Google Scholar
Merbs, S.L. & Nathans, J. (1992). Absorption spectra of the hybrid pigments responsible for anomalous color vision. Science 258, 464–466.CrossRefGoogle Scholar
Neitz, M., Neitz, J., & Jacobs, G.H. (1995). Genetic basis of photopigment variations in human dichromats. Vision Research 35, 2095–2103.CrossRefGoogle Scholar
Ogden, T.E. (1973). The oscillatory waves of the primate electroretinogram. Vision Research 13, 1059–1074.CrossRefGoogle Scholar
Pokorny, J. & Smith, V. (1987). L/M Cone ratios and the null point of the perceptual red/green opponent system. Die Farbe 34, 53–57.Google Scholar
Rangaswamy, N., Hood, D.C., & Frishman, L.J. (2003) from if Regional variation in local contributions to the photopic flash ERG revealed using the slow-sequence mfERG. Investigative Ophthalmology & Visual Science 44, 3233–3247.CrossRefGoogle Scholar
Stockman, A. & Sharpe, L.T. (2000). The spectral sensitivities of the middle- and long-wavelength sensitive cones derived from measurements in observers of known genotype. Vision Research 40, 1711–1737.CrossRefGoogle Scholar
Sharpe, L.T., Stockman, A., Jägle, H., & Nathans, J. (1999). Cone photopigments, opsin genes and retinal mosaics. In Color vision: From genes to perception, ed. Gegenfurtner, K. & Sharpe, L.T., pp. 3–51. Cambridge, Cambridge University Press.
Sharpe, L.T., Stockman, A., Jägle, H., Knau, H., Klausen, G., Reitner, A., & Nathans, J. (1998). Red, green and red–green hybrid pigments in the human retina: Correlations between deduced protein sequences and psychophysically measured spectral sensitivities. Journal of Neuroscience 18, 10053–10069.Google Scholar
Sutter, E.E. & Tran, D. (1992). The field topography of ERG components in man. I. The photopic luminance response. Vision Research 32, 433–446.Google Scholar
Wu, S. & Sutter, E.E. (1995). A topographic study of oscillatory potentials in man. Visual Neuroscience 12, 1013–1025.CrossRefGoogle Scholar