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Neonatal administration of the neurotoxin 5,7-dihydroxytryptamine results in synaptic reorganization in the superficial gray layer of the hamster's superior colliculus

Published online by Cambridge University Press:  02 June 2009

Erick A. Arce ,
Robert W. Rhoades  and
Richard D. Mooney
Erick A. Arce
Affiliation:
Department of Anatomy, Medical College of Ohio, Toledo
Robert W. Rhoades
Affiliation:
Department of Anatomy, Medical College of Ohio, Toledo
Richard D. Mooney
Affiliation:
Department of Anatomy, Medical College of Ohio, Toledo
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Abstract

Neonatal subcutaneous administration of the neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) to hamsters results in a marked depletion of serotonin (5-HT) in cortex and an increase in the concentration of this amine in the superior colliculus (SC). To determine whether this increase was associated with an alteration in the synaptic organization of 5-HT-containing axons in the superficial gray layer of the SC, immunocytochemistry was combined with electron microscopy. In normal adult hamsters, only 4.0% of 500 5-HT-immunoreactive profiles make synaptic contacts in the superficial gray layer of the hamster's SC. In 5,7-DHT-treated animals, examination of 400 individual profiles indicated that 25.5% of 5-HT-positive profiles made synaptic contacts (P < 0.05). Given the recently demonstrated effect of 5-HT on retinotectal transmission in this species, the present results suggest that the functional organization of the SC may also be markedly altered in animals that sustain neonatal 5,7-DHT administration.

Keywords

SerotoninImmunocytochemistry5, 7-DihydroxytryptamineElectron microscopy
Type
Research Articles
Information
Visual Neuroscience , Volume 12 , Issue 4 , July 1995 , pp. 779 - 783
Copyright
Copyright © Cambridge University Press 1995

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