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Neurotensin induces calcium oscillations in cultured amacrine cells

Published online by Cambridge University Press:  02 June 2009

Salvador Borges ,
Evanna Gleason ,
Matthew Frerking  and
Martin Wilson
Salvador Borges
Affiliation:
Division of Biological Sciences, Section of Neurobiology, Physiology and Behavior, University of California, Davis
Evanna Gleason
Affiliation:
Division of Biological Sciences, Section of Neurobiology, Physiology and Behavior, University of California, Davis
Matthew Frerking
Affiliation:
Division of Biological Sciences, Section of Neurobiology, Physiology and Behavior, University of California, Davis
Martin Wilson
Affiliation:
Division of Biological Sciences, Section of Neurobiology, Physiology and Behavior, University of California, Davis
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Abstract

The peptide, neurotensin, is found in a class of amacrine cells synapsing chiefly withother amacrine cells in the chicken retina (Li & Lam, 1990; Watt et al., 1991). Toinvestigate the possible effects of neurotensin, we have used Ca2+ imaging to measure cytosolic Ca2+ concentrations in cultured chick amacrine cells. Following a delay of about 2 min, neurotensin (300 nM) induced oscillations in Ca2+ concentration that typically had a period of 2 min and peak values of about 300 nM when averaged over the cell body. The phospholipase C inhibitors U-73, 112 and 4′-bromophenacyl bromide terminated oscillations induced by neurotensin but the protein kinase inhibitors H7 and staurosporine did not inhibit oscillations, increasing their frequency instead. In the absence of external Ca2+, neurotensin induced only a single Ca2+ transient, much briefer than when external Ca2+ was present. Together these results suggest that neurotensin activates phospholipase C, thereby producing IP3 that triggers Ca2+ release from an internal store. Although this released Ca2+ contributes to periodic Ca2+ peaks, the majority of cytosolic Ca2+, even in the first peak, comes from Ca2+ influx across the plasmalemma.

Keywords

RetinaNeurotensinCalciumCalcium oscillationPhospholipase CAmacrine cell
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 2 , March 1996 , pp. 311 - 318
Copyright
Copyright © Cambridge University Press 1996

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