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Pigment granule migration in crustacean photoreceptors requires calcium

Published online by Cambridge University Press:  02 June 2009

Christina King-Smith  and
Thomas W. Cronin
Christina King-Smith
Affiliation:
Department of Biological Sciences, University of Maryland – Baltimore County, Baltimore
Thomas W. Cronin
Affiliation:
Department of Biological Sciences, University of Maryland – Baltimore County, Baltimore
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Abstract

We have investigated the role of calcium in the regulation of pigment granule migration in photoreceptors of the semi-terrestrial crab, Sesarma cinereum. Isolated crab eyes (eyecup plus eyestalk) were maintained in crustacean Ringer either prepared normally or calcium-free plus 50 mM EGTA. Pigment granule movement was indirectly observed by monitoring reflectance from the eye during light stimuli using intracellular optical physiological techniques. Electroretinograms (ERGs) were also measured during light stimuli. EGTA treatment caused gradual loss of centripetal migration of pigment granules (normally leading to pupillary closure), and photoreceptors eventually became locked in the open-pupil, dark-adapted state despite repeated stimuli. In contrast, ERG responses continued throughout EGTA treatment, although the size and shape ofthe response was altered. Normal ERG responses and pigment granule movements returned after replacing EGTA-Ringer with normal-calcium medium. These results suggest that centripetal migration of pigment granules in crustacean photoreceptors requires calcium.

Keywords

Pigment granule migrationInvertebrate photoreceptorsExtracellular calciumElectroretinogramIntracellular optical physiology
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 1 , January 1996 , pp. 43 - 49
Copyright
Copyright © Cambridge University Press 1996

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