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Requirement of N-linked glycosylation site in Drosophila rhodopsin

Published online by Cambridge University Press:  02 June 2009

J. E. O'Tousa
J. E. O'Tousa
Affiliation:
Department of Biological Sciences, University of Notre Dame, Notre Dame
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Abstract

In vitro mutagenesis and germline transformation were used to create a Drosophila mutant, ΔAsn20 lacking the N-linked glycosylation site near the amino terminus of the major rhodopsin (Asn20-Gly-Ser changed to Ile-Gly-Ser). Low opsin protein levels are detected in ΔAsn20 photoreceptors. Electroretinogram responses of mutant flies show that the residual rhodopsin found in this mutant is capable of initiating phototransduction. The organization of rhabdomeres, the photoreceptor organelle containing nearly all of the rhodopsin, is aberrant in the ΔAsn20 mutant and undergoes age-dependent deterioration. These results establish that an N-linked glycosylation site, and likely glycosylation itself, plays a critical role in the maturation of Drosophila rhodopsin.

Keywords

RhodopsinN-linked glycosylationPhotoreceptorVisual pigmentsDrosophila
Type
Research Articles
Information
Visual Neuroscience , Volume 8 , Issue 5 , May 1992 , pp. 385 - 390
Copyright
Copyright © Cambridge University Press 1992

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