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Reversal potential of GABA-induced currents in rod bipolar cells of the rat retina

Published online by Cambridge University Press:  02 June 2009

Masayuki Yamashita  and
Heinz Wässle
Masayuki Yamashita
Affiliation:
Max-Planck-Institut fü Hirnforschung, Deutschordenstrasse 46, D-6000 Frankfurt 71, Germany
Heinz Wässle
Affiliation:
Max-Planck-Institut fü Hirnforschung, Deutschordenstrasse 46, D-6000 Frankfurt 71, Germany
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Abstract

GABA-induced whole-cell currents were measured in rod bipolar cells dissociated from the adult rat retina. The patch-clamp electrode contained nystatin, which made the cell membrane electrically permeable without rupture, thus retarding the rate of diffusion of Cl– ions from the patch pipette to the cell interior. The reversal potential of the GABA-induced currents was around –70 mV at an extracellular Cl-–concentration of 149 mM. We conclude that GABA generates hyperpolarizing responses in rod bipolar cells of the rat retina.

Keywords

RatRetinaRod bipolar cellGamman-aminobutyic acidReversal potentialChlorideNystatin
Type
Short Communication
Information
Visual Neuroscience , Volume 6 , Issue 4 , April 1991 , pp. 399 - 401
Copyright
Copyright © Cambridge University Press 1991

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