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A second episode of ganglion cell death takes place when an optic nerve regenerates for a second time in the frog

Published online by Cambridge University Press:  02 June 2009

L. D. Beazley  and
J.E. Darby
L. D. Beazley
Affiliation:
Neurobiology Laboratory, Department of Psychology, The University of Western Australia, Nedlands, Australia
J.E. Darby
Affiliation:
Neurobiology Laboratory, Department of Psychology, The University of Western Australia, Nedlands, Australia
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Abstract

We have previously reported that during optic nerve regeneration in the frog, 30–40% of retinal ganglion cells die, the loss being complete within 10 weeks. In the present study, we crushed the optic nerve, waited 10 weeks, and then recrushed the nerve at the same site. Retinae were examined 10 weeks later. We estimated ganglion cell numbers from cresyl-violet-stained wholemounts and found a fall of 53% compared to normals. The loss was significantly greater than the losses of 36% and 35%, respectively, in frogs which received a single optic nerve crush and were examined 10 or 20–24 weeks later. The results indicate that a second episode of ganglion cell death took place when the optic nerve regenerated a second time. We conclude that ganglion cells in the frog are not comprised of two subpopulations, only one of which intrinsically possesses the ability to regenerate.

Keywords

FrogOptic nerve regenerationGanglion cell deathRepeated optic nerve crush
Type
Research Article
Information
Visual Neuroscience , Volume 10 , Issue 2 , March 1993 , pp. 297 - 301
Copyright
Copyright © Cambridge University Press 1993

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