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Similar properties of cGMP-activated channels between cones and rods in the carp retina

Published online by Cambridge University Press:  02 June 2009

Shu-Ichi Watanabe  and
Motohiki Murakami
Shu-Ichi Watanabe
Affiliation:
Department of Physiology, Keio University of Medicine, Shinjuki-ju, Tokoyo, 160 Japan
Motohiki Murakami
Affiliation:
Department of Physiology, Keio University of Medicine, Shinjuki-ju, Tokoyo, 160 Japan
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Abstract

Using patch-clamp techniques, properties of cGMP-activated channel were studied at a single-channel level in order to examine (1) whether any differences are recognized between the cGMP-activated channels of rods and cones in the same animal species, and (2) whether the channel properties of the same photoreceptor class differ in different animal species. Experiments were performed on inside-out membrane patches excised from outer segments of rods and morphological subtypes of cones in the carp retina. Single-channel activities could be recorded when the patches were perfused with low concentrations of cGMP (<10 μM). Throughout five morphological subtypes of cones and rod, single-channel currents showed no significant rectification at membrane hyperpolarization in a low divalent cation solution, and single-channel conductances were almost the same: 13.8 ± 0.2 pS (mean ± s.e.m., n = 23) in cones and 12.7 ± 0.8 pS (n = 3) in rods. These values were significantly smaller than that reported in catfish cones (about 50 pS), and that in rods of the toad and the tiger salamander (about 25 pS). In rods and all subtypes of cones of the carp, open durations of cGMP activated channels were brief. In addition, kinetic parameters of channel openings and closings showed no differences throughout all subtypes of cones and rod.

Keywords

RetinaPhotoreceptorIon channelsCyclic GMPCarp
Type
Research Articles
Information
Visual Neuroscience , Volume 6 , Issue 6 , June 1991 , pp. 563 - 568
Copyright
Copyright © Cambridge University Press 1991

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