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The Allelopathic Potential of Kudzu (Pueraria montana)

Published online by Cambridge University Press:  20 January 2017

Md H. Rashid ,
Takashi Asaeda  and
Md N. Uddin
Md H. Rashid
Affiliation:
Department of Environmental Science and Technology, Saitama University, 255 Shimo–okubo, Sakura, Saitama 338–8570, Japan
Takashi Asaeda*
Affiliation:
Department of Environmental Science and Technology, Saitama University, 255 Shimo–okubo, Sakura, Saitama 338–8570, Japan
Md N. Uddin
Affiliation:
Department of Environmental Science and Technology, Saitama University, 255 Shimo–okubo, Sakura, Saitama 338–8570, Japan
*
Corresponding author's E-mail: asaeda@mail.saitama-u.ac.jp
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Abstract

We explored the allelopathic potential of kudzu as a function of its phenolics. Aqueous and methanol extracts of different kudzu organs (leaf, stem, root, and seed) were assayed for allelopathy with the use of lettuce and radish seeds. Both leaf and root extracts significantly inhibited all of the measured germination indices (total germination, speed of germination, and coefficient of the rate of germination) (all P < 0.01). When treated with leaf extract, the total germination of both species was ∼ 20% less than the control. Furthermore, the leaf extract significantly reduced the speed of germination to 38 and 53% that of the lettuce and radish controls, respectively. Lettuce and radish seeds soaked in leaf and root extracts for 24 h imbibed less water (∼ 30% for both species) than those soaked in distilled water (control), suggesting that a reduction of water imbibition might be one of the mechanisms of germination retardation. Stem and seed extracts affected neither the water uptake nor the germination indices of radish and lettuce seeds. Kudzu leaves and roots contain higher amounts of total phenolics (P = 0.001) and soluble phenolics (P = 0.005) than stems and seeds, consistent with the results of the germination bioassays. In agar plate bioassays, both litter and rhizosphere soil had phytotoxic effects on the radicle growth of radish (P = 0.003) and perennial ryegrass (P = 0.001) seedlings. Perennial ryegrass and cobbler's pegs seedlings grown on leaf and root leachate-amended soil gave ∼ 40% shorter roots and shoots and ∼ 50% less dry weight than those grown in leachate-free soil. Kudzu litter was incorporated and incubated in soil for 6 wk, at which point the soluble phenolics in the soil solution were at a level (> 150 ppm) considered to be allelopathic.

Keywords

Cobbler's pegs, Bidens pilosa L.kudzu, Pueraria montana (Lour.)perennial ryegrass, Lolium perenne L.lettuce, Lactuca sativa L.radish, Raphanaus sativus L.Allelopathylitter decompositionphenolicsseedling growthsoil system
Type
Weed Biology and Ecology
Information
Weed Science , Volume 58 , Issue 1 , March 2010 , pp. 47 - 55
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Al-Humaid, A. I. and Warrag, M. O. A. 1998. Allelopathic effects of mesquite (Prosopis juliflora) foliage on seed germination and seedling growth of bermudagrass (Cynodon dactylon). J. Arid Environ. 38:237–243.CrossRefGoogle Scholar
Allaie, R. R., Reshi, Z., Rashid, I., and Wafai, B. A. 2006. Effect of aqueous leaf leachate of Anthemis cotula—an alien invasive species on germination behaviour of some field crops. J. Agron. Crop Sci. 192:186–191.Google Scholar
Anjum, T. and Bajwa, R. 2005. Importance of germination indices in interpretation of allelochemical effects. Int. J. Agric. Biol. 7:417–419.Google Scholar
Bais, H. P., Vepachedu, R., Gilroy, S., Callaway, R. M., and Vivanco, J. M. 2003. Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science. 301:1377–1380.Google Scholar
Bewley, J. D. and Black, M. 1985. Seeds: Physiology of Development and Germination. 1st ed. New York Plenum Press. 367.CrossRefGoogle Scholar
Bradbeer, J. W. 1988. Seed Dormancy and Germination. London Blackie and Son. 146.Google Scholar
Callaway, R. M. 2002. The detection of neighbors by plants. Trends Ecol. Evol. 17:104–105.Google Scholar
Callaway, R. M. and Aschehoug, E. T. 2000. Invasive plants versus their new and old neighbours: a mechanism for exotic invasion. Science. 240:521–523.Google Scholar
Chen, L., Wang, S., and Yu, X. 2005. Effects of phenolics on seedling growth and 15N nitrate absorption of Cunninghamia lanceolata . Allelopathy J. 15:57–66.Google Scholar
Chiapusio, G., Sánchez, A. M., Reigosa, M. J., González, L., and Pellissier, F. 1997. Do germination indices adequately reflect allelochemical effects on the germination process? J. Chem. Ecol. 23:2445–2453.CrossRefGoogle Scholar
Chou, C. H. and Patrick, Z. A. 1976. Identification and phytotoxic activity of compounds produced during decomposition of corn and rye residues in soil. J. Chem. Ecol. 2:369–387.Google Scholar
Emeterio, L. S., Arroyo, A., and Canals, R. M. 2004. Allelopathic potential of Lolium rigidum Gaud. on the early growth of three associated pasture species. Grass Forage Sci. 59:107–112.CrossRefGoogle Scholar
Fujii, Y., Furubayashi, A., and Hiradate, S. 2005. Rhizosphere soil method: a new bioassay to evaluate allelopathy in the field. The Fourth World Congress on Allelopathy. Available via DIALOG. http://www.regional.org.au/au/allelopathy/2005/2/3/2535_fujiiy.htm. Accessed: April 1, 2009.Google Scholar
Fujii, Y., Shibuya, T., Nakatani, K., Itani, T., Hiradate, S., and Parvez, M. M. 2004. Assessment method for allelopathic effect from leaf litter leachates. Weed Biol. Manag. 4:19–23.Google Scholar
Fujii, Y., Shibuya, T., and Usami, Y. 1991. Allelopathic effect of Mucuna pruriens on the appearance of weeds. Weed Res. Japan. 36:43–49. [In Japanese with English summary].Google Scholar
Geissman, T. A. and Crout, D. H. G. 1969. Organic Chemistry of Secondary Plant Metabolism. 1st ed. San Francisco Freeman-Cooper. 429.Google Scholar
Hoffman, M., Weston, L. A., Snyder, J. C., and Regnier, E. E. 1996. Allelopathic influence of germinating seeds and seedlings of cover crops on weed species. Weed Sci. 44:579–584.Google Scholar
Horner, J. D., Gosz, J. R., and Cates, R. G. 1988. The role of carbon-based plant secondary metabolites in decomposition in terrestrial ecosystems. Am. Nat. 132:869–883.Google Scholar
Inderjit, , 1996. Plant phenolics in allelopathy. Bot. Rev. 62:182–202.Google Scholar
Inderjit, , Asakawa, C., and Dakshini, K. M. M. 1999. Allelopathic potential of Verbesina encelioides root leachate in soil. Can. J. Bot. 77:1419–1424.Google Scholar
Inderjit, , and Dakshini, K. M. M. 1999. Bioassay for allelopathy: interaction of soil organic and inorganic constituents. Pages 35–44. in Inderjit, , Dakshini, K. M. M., and Foy, C. F. Principles and Practices in Plant Ecology: Allelochemical Interactions. New York CRC.Google Scholar
Inderjit, , and Foy, C. L. 1999. Nature of interference potential of mugwort (Artemisia vulgaris). Weed Technol. 13:176–182.Google Scholar
Inderjit, , and Weiner, J. 2001. Plant allelochemical interference or soil chemical ecology? Perspect. Plant Ecol. Evol. System. 4:3–12.Google Scholar
Iqbal, Z., Hiradate, S., Noda, A., Isojima, S., and Fujii, Y. 2003. Allelopathic activity of buckwheat: isolation and characterization of phenolics. Weed Sci. 51:657–662.Google Scholar
Johnson, G. N., Young, A. J., Scholes, J. D., and Horton, P. 1993. The dissipation of excess excitation energy in British plant species. Plant Cell Environ. 16:673–679.Google Scholar
Kamo, T., Hiradate, S., and Fujii, Y. 2003. First isolation of natural cyanamide as a possible allelochemical from hairy vetch Vicia vilosa . J. Chem. Ecol. 29:275–283.Google Scholar
Kato-Noguchi, H. 2003a. Allelopathic potential of Pueraria thunbergiana . Physiol. Plant. 47:471–473.Google Scholar
Kato-Noguchi, H. 2003b. Allelopathic substances in Pueraria thunbergiana . Phytochemistry. 63:577–580.Google Scholar
Kirakosyan, A., Kaufman, P. B., Warber, S., Bolling, S., and Duke, J. A. 2003. Quantification of major isoflavonoids and L-canavanine in several organs of kudzu vine (Pueraria montana) and in starch samples derived from kudzu roots. Plant Sci. 164:883–888.Google Scholar
Kumar, V., Brainard, D. C., and Bellinder, R. R. 2009. Suppression of Powell amaranth (Amaranthus powellii) by buckwheat residues: role of allelopathy. Weed Sci. 57:66–73.Google Scholar
Macias, F. A., Castellano, D., and Molinillo, J. M. G. 2000. Search for a standard phytotoxic bioassay for allelochemicals. Selection of target species. J. Agric. Food Chem. 48:2512–2521.Google Scholar
Marwood, C. A., Bestari, K. T., Gensemer, R. W., Solomon, K. R., and Greenberg, B. M. 2003. Chlorophyll fluorescence as a bioindicator of creosote toxicity to plant growth in aquatic microcosms. Environ. Toxicol. Chem. 22:1075–1085.CrossRefGoogle ScholarPubMed
Maxwell, K. and Johnson, G. N. 2000. Chlorophyll fluorescence—a practical guide. J. Exp. Bot. 51:659–668.Google Scholar
Moran, R. 1982. Formulae for determination of chlorophyllous pigments extracted with N, N-dimethylformamide. Plant Physiol. 69:1376–1381.Google Scholar
Moran, R. and Porath, D. 1980. Chlorophyll determination in intact tissues using N, N dimethylformamide. Plant Physiol. 65:478–479.Google Scholar
Pandey, D. K. 1996. Phytotoxicity of sesquiterpene lactone parthenin on aquatic weeds. J. Chem. Ecol. 22:151–160.Google Scholar
Parks, L. J., Tanner, R. D., and Prokop, A. 2002. Kudzu (Pueraria lobata), a valuable potential commercial resource: food, paper, textiles and chemicals. Pages 259–272. in Keung, W. M. Pueraria: the genus Pueraria. London Taylor and Francis.Google Scholar
Pue, K. J., Blum, U., Gerig, T. M., and Shafer, S. R. 1995. Mechanism by which noninhibitory concentration of glucose increases inhibitory activity of p-coumaric acid on morning-glory seedling biomass accumulation. J. Chem. Ecol. 21:833–847.Google Scholar
Putnam, A. R. and Tang, C. S. 1986. Allelopathy: state of the science. Pages 1–19. in Putnam, A. R. and Tang, C. S. The Science of Allelopathy. New York John Wiley & Sons.Google Scholar
Quayyum, H. A., Mallik, A. U., and Lee, P. F. 1999. Allelopathic potential of aquatic plants associated with wild rice (Zizania palustris): I. bioassay with plant and lake sediment samples. J. Chem. Ecol. 25:209–220.CrossRefGoogle Scholar
Reigosa, M. J. and Pazos-Malvido, E. 2007. Phytotoxic effects of 21 plant secondary metabolites on Arabidopsis thaliana germination and root growth. J. Chem. Ecol. 22:1456–1466.Google Scholar
Rice, E. L. 1984. Allelopathy. 2nd ed. New York Academic Press. 368.Google Scholar
Roháček, K. and Barták, M. 1999. Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica. 37:339–363.Google Scholar
Sampietro, D. A., Vattuone, M. A., and Isla, M. I. 2006. Plant growth inhibitors isolated from sugarcane (Saccharum officinarum) straw. J. Plant Physiol. 163:837–846.Google Scholar
Shofield, J. A., Hagerman, A. E., and Harold, A. 1998. Loss of tannin and other phenolics from willow leaf litter. J. Chem. Ecol. 24:1409–1421.Google Scholar
Siddiqui, Z. S. 2007. Allelopathic effects of black pepper leachings on Vigna mungo (L.) Hepper. Acta Physiol. Plant. 29:303–308.Google Scholar
Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am. J. Enol. Vitic. 16:144–158.Google Scholar
Stewart, L. 2005. Allelopathic study of Pueraria lobata on the germination of Quercus palustris and selected vegetables. Oklahoma Junior Academy of Science. Available via DIALOG. http://oas.ucok.edu/OJAS/05/paper/stewart.htm. Accessed: April 1, 2009.Google Scholar
Stowe, R. P., Koenig, D. W., Mishra, S. K., and Pierson, D. L. 1995. Nondestructive and continuous spectrophotometric measurement of cell respiration using a tetrazolium–formazan microemulsion. J. Microbiol. Methods. 22:283–292.Google Scholar
Tawaha, A. M. and Turk, M. A. 2003. Allelopathic effects of black mustard (Brassica nigra) on germination and growth of wild barley (Hordeum spontaneum). J. Agron. Crop Sci. 189:298–303.Google Scholar
Thijs, H., Shann, J. D., and Weidenhamer, J. D. 1994. The effect of phytotoxins on competitive outcome in a model system. Ecology. 75:1959–1964.Google Scholar
Tsanuo, M. K., Hassanali, A., Hooper, A. M., Khan, Z., Kaberia, F., Pickett, J. A., and Wadhams, L. J. 2003. Isoflavanones from the allelopathic aqueous root exudate of Desmodium uncinatum . Phytochemistry. 64:265–273.Google Scholar
Turk, M. A. and Tawaha, A. M. 2002. Inhibitory effects of aqueous extracts of black mustard on germination and growth of lentil. Pak. J. Agron. 1:28–30.Google Scholar
Uraguchi, S., Watanabe, I., Kuno, K., Hoshino, Y., and Fujii, Y. 2003. Allelopathy of floodplain vegetation species in the middle course of Tama River. J. Weed Sci. Technol. 48:117–129. [In Japanese with English summary].Google Scholar
Wardle, D. A., Ahmed, M., and Nicholson, K. S. 1991. Allelopathic influence of nodding thistle (Carduusnutans nutans L.) seeds on germination and radicle growth of pasture plants. N. Z. J. Agric. Res. 34:185–191.Google Scholar
Whitehead, D. C., Dibb, H., and Hartley, R. D. 1981. Extractant pH and the release of phenolic compounds from soil, plant roots and leaf litter. Soil Biol. Biochem. 13:343–348.Google Scholar