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Carbohydrate, phytohormone, and associated transcriptome changes during storage root formation in alligatorweed (Alternanthera philoxeroides)

Published online by Cambridge University Press:  11 May 2020

Junliang Yin Open the ORCID record for Junliang Yin [Opens in a new window] ,
Jie Tian ,
Gang Li ,
Yongxing Zhu ,
Xiaokang Zhou ,
Yang He ,
Peiyao Nie ,
Yanmeng Su ,
Qiwen Zhong  and
Zhongyi Chen
Junliang Yin
Affiliation:
Researcher, Academy of Agriculture and Forestry Sciences of Qinghai University (Qinghai Academy of Agriculture and Forestry Sciences), Qinghai Key Laboratory of Vegetable Genetics and Physiology, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China Researcher, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China
Jie Tian
Affiliation:
Researcher, Academy of Agriculture and Forestry Sciences of Qinghai University (Qinghai Academy of Agriculture and Forestry Sciences), Qinghai Key Laboratory of Vegetable Genetics and Physiology, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China
Gang Li
Affiliation:
Researcher, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China
Yongxing Zhu
Affiliation:
Researcher, Academy of Agriculture and Forestry Sciences of Qinghai University (Qinghai Academy of Agriculture and Forestry Sciences), Qinghai Key Laboratory of Vegetable Genetics and Physiology, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China Researcher, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China
Xiaokang Zhou
Affiliation:
Researcher, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China
Yang He
Affiliation:
Researcher, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China
Peiyao Nie
Affiliation:
Researcher, Biomarker Technologies, Beijing, China
Yanmeng Su
Affiliation:
Researcher, Suzhou Grace Biotechnology Co. Ltd., Suzhou, China
Qiwen Zhong*
Affiliation:
Professor, Academy of Agriculture and Forestry Sciences of Qinghai University (Qinghai Academy of Agriculture and Forestry Sciences), Qinghai Key Laboratory of Vegetable Genetics and Physiology, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China
Zhongyi Chen*
Affiliation:
Professor, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China
*
Authors for correspondence: Qiwen Zhong, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining810016, China. (Email: jerusalemyys@aliyun.com) Zhongyi Chen, College of Horticulture and Gardening, Yangtze University, Jingzhou434000, Hubei, China. (Email: czy@yangtzeu.edu.cn)
Authors for correspondence: Qiwen Zhong, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining810016, China. (Email: jerusalemyys@aliyun.com) Zhongyi Chen, College of Horticulture and Gardening, Yangtze University, Jingzhou434000, Hubei, China. (Email: czy@yangtzeu.edu.cn)
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Abstract

The storage root of alligatorweed [Alternanthera philoxeroides (Mart.) Griseb.] growing in terrestrial habitats is an important metamorphic organ for its propagation, overwintering, and spread. However, the regulatory mechanism adventitious root expansion to form storage roots is still unclear. To reveal the changes accompanying the root-swelling process, we quantified sugar, soluble protein, and phytohormone content in adventitious and storage roots. Results demonstrated that sucrose, fructose, and soluble protein increased in storage roots, whereas abscisic acid (ABA), indoleacetic acid (IAA), brassinosteroid (BR), gibberellin, jasmonic acid, and cytokinin (trans-zeatin [tZ] and isopentenyladenine [iP] and the corresponding ribosides tZR and iPR). tZ-type (tZR and tZ) content decreased, suggesting the involvement of sugars and hormones in the formation of storage roots. To further reveal the molecular basis of A. philoxeroides’s ability to form storage roots and provide candidate genes for molecular function analyses, we assembled a de novo transcriptome of A. philoxeroides based on four sets of RNA-sequencing data. According to functional annotation and expression profiling, 42 unigenes involved in sucrose synthesis and hydrolysis were identified, in addition to 70, 58, and 78 unigenes in ABA, BR, and IAA signal transduction, respectively. The quantitative reverse transcriptase polymerase chain reaction analysis revealed 21 unigenes involved in sugar metabolism and hormone signal transduction were differentially expressed during the formation of storage roots. These results revealed metabolic changes during the formation of storage roots and provide candidate genes involved in sugar and phytohormone metabolism in A. philoxeroides.

Keywords

Alligator weedbioinformaticssignaling transductionsugar metabolismroot swelling

Information

Type
Research Article
Information
Weed Science , Volume 68 , Issue 4 , July 2020 , pp. 382 - 395
Copyright
© Weed Science Society of America, 2020

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Footnotes

Associate Editor: William Vencill, University of Georgia

*

These authors contributed equally to this work.

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