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Multiple Resistance to Glyphosate and Pyrithiobac in Palmer Amaranth (Amaranthus palmeri) from Mississippi and Response to Flumiclorac

Published online by Cambridge University Press:  20 January 2017

Vijay K. Nandula ,
Krishna N. Reddy ,
Clifford H. Koger ,
Daniel H. Poston ,
Agnes M. Rimando ,
Stephen O. Duke ,
Jason A. Bond  and
Daniela N. Ribeiro
Vijay K. Nandula*
Affiliation:
Crop Production Systems Research Unit, USDA-ARS, Stoneville, MS 38776
Krishna N. Reddy
Affiliation:
Crop Production Systems Research Unit, USDA-ARS, Stoneville, MS 38776
Clifford H. Koger
Affiliation:
Syngenta Crop Protection Inc., 112 Meadowlark Lane, Indianola, MS 38751
Daniel H. Poston
Affiliation:
Pioneer Hi-Bred International–Southern Business Unit, 700 Boulevard S, SW, Suite 302, Huntsville, AL 35802
Agnes M. Rimando
Affiliation:
Natural Products Utilization Research Unit, USDA-ARS, University, MS 38677
Stephen O. Duke
Affiliation:
Natural Products Utilization Research Unit, USDA-ARS, University, MS 38677
Jason A. Bond
Affiliation:
Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776
Daniela N. Ribeiro
Affiliation:
Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS 39762
*
Corresponding author's E-mail: vijay.nandula@ars.usda.gov
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Abstract

Greenhouse and laboratory studies were conducted to confirm and quantify glyphosate resistance, quantify pyrithiobac resistance, and investigate interaction between flumiclorac and glyphosate mixtures on control of Palmer amaranth from Mississippi. The GR50 (herbicide dose required to cause a 50% reduction in plant growth) values for two glyphosate-resistant biotypes, C1B1 and T4B1, and a glyphosate-susceptible (GS) biotype were 1.52, 1.3, and 0.09 kg ae ha−1 glyphosate, respectively. This indicated that the C1B1 and T4B1 biotypes were 17- and 14-fold resistant to glyphosate, respectively, compared with the GS biotype. The C1B1 and T4B1 biotypes were also resistant to pyrithiobac, an acetolactate synthase (ALS) inhibitor, with GR50 values of 0.06 and 0.07 kg ai ha−1, respectively. This indicated that the C1B1 and T4B1 biotypes were 7- and 8-fold, respectively, more resistant to pyrithiobac compared with the GS biotype, which had a GR50 value of 0.009 kg ha−1. Flumiclorac was antagonistic to glyphosate by reducing glyphosate translocation. The C1B1 and T4B1 absorbed less glyphosate 48 h after treatment (HAT) compared with the GS biotype. The majority of the translocated glyphosate accumulated in the shoot above the treated leaf (that contains the apical meristem) in the GS biotype and in the shoot below the treated leaf in the resistant biotypes, C1B1 and T4B1, by 48 HAT. The C1B1 biotype accumulated negligible shikimate levels, whereas the T4B1 and GS biotypes recorded elevated levels of shikimate. Metabolism of glyphosate to aminomethylphosphonic acid was not detected in either of the resistant biotypes or the susceptible GS biotype. The above results confirm multiple resistance to glyphosate and pyrithiobac in Palmer amaranth biotypes from Mississippi and indicate that resistance to glyphosate is partly due to reduced absorption and translocation of glyphosate.

Keywords

FlumicloracglyphosatepyrithiobacPalmer amaranth, Amaranthus palmeri S. Wats. AMAPAAbsorptionAMPAantagonismshikimatetranslocation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 60 , Issue 2 , June 2012 , pp. 179 - 188
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Anonymous. 2006. Resource® herbicide label. Walnut Creek, CA Valent U.S.A. Corporation.Google Scholar
Anonymous. 2009. Roundup WeatherMAX® herbicide label. St. Louis, MO Monsanto Company.Google Scholar
Burke, I. C., Koger, C. H., Reddy, K. N., and Wilcut, J. W. 2007. Reduced translocation is the cause of antagonism of glyphosate by MSMA in browntop millet (Brachiaria ramosa) and Palmer amaranth (Amaranthus palmeri). Weed Technol. 21:166–170.Google Scholar
Colby, S. R. 1967. Calculating synergistic and antagonistic response of herbicide combinations. Weeds. 15:20–22.Google Scholar
Cromartie, T. H. and Polge, N. D. 2000. An improved assay for shikimic acid and its use as a monitor for the activity of sulfosate. Abstr. Weed Sci. Soc. Am. 40:291.Google Scholar
Culpepper, A. S., Grey, T. L., Vencill, W. K., Kichler, K. M., Webster, T. M., Brown, S. M., York, A. C., Davis, J. M., and Hanna, W. W. 2006. Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) confirmed in Georgia. Weed Sci. 54:620–626.Google Scholar
Duke, S. O. 2011. Glyphosate metabolic degradation in glyphosate resistant crops and weeds vs. susceptible crops and weeds. J. Agric. Food Chem. 59:5835–5841.Google Scholar
Duke, S. O., Baerson, S. R., and Rimando, A. M. 2003. Herbicides: glyphosate. in Plimmer, J. R., Gammon, D. W., and Ragsdale, N. N., eds. Encyclopedia of Agrochemicals. New York Wiley. http://www.mrw.interscience.wiley.com/eoa/articles/agr119/frame.html. Accessed: August 26, 2011.Google Scholar
Franz, J. E., Mao, M. K., and Sikorski, J. A. 1997. Glyphosate: A Unique Global Herbicide. Washington, DC American Chemical Society Monograph 189. 653 p.Google Scholar
Gaines, T. A., Zhang, W., Wang, D., et al. 2010. Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc. Natl. Acad. Sci. U.S.A. 107:1029–1034.Google Scholar
Heap, I. M. 2011. International Survey of Herbicide Resistant Weeds. www.weedscience.org. Accessed: September 19, 2011.Google Scholar
Koger, C. H., Burke, I. C., Miller, D. K., Kendig, J. A., Reddy, K. N., and Wilcut, J. W. 2007. MSMA antagonizes glyphosate and glufosinate efficacy on broadleaf and grass weeds. Weed Technol. 21:159–165.Google Scholar
Koger, C. H. and Reddy, K. N. 2005. Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis). Weed Sci. 53:84–89.Google Scholar
Nandula, V. K., Reddy, K. N., Poston, D. H., Rimando, A. M., and Duke, S. O. 2008. Glyphosate tolerance mechanism in Italian ryegrass (Lolium multiflorum) from Mississippi. Weed Sci. 56:344–349.Google Scholar
Norsworthy, J. K., Griffith, G. M., Scott, R. C., Smith, K. L., and Oliver, L. R. 2008a. Confirmation and control of glyphosate-resistant Palmer amaranth (Amaranthus palmeri) in Arkansas. Weed Technol. 22:108–113.Google Scholar
Norsworthy, J. K., Scott, R. C., Smith, K. L., and Oliver, L. R. 2008b. Response of northeastern Arkansas Palmer amaranth (Amaranthus palmeri) accessions to glyphosate. Weed Technol. 22:408–413.Google Scholar
Pratley, J., Baines, P., Eberbach, P., Incerti, M., and Broster, J. 1996. Glyphosate resistance in annual ryegrass. Page 126 in Virgona, J., and Michalk, D., eds. Proceedings of the 11th Annual Conference of the Grasslands Society of New South Wales. Wagga Wagga, Australia The Grasslands Society of NSW.Google Scholar
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Kruger, R. 1999. Resistance to glyphosate in Lolium rigidum . I. Bioevaluation. Weed Sci. 47:405–411.Google Scholar
Reddy, K. N., Rimando, A. M., Duke, S. O., and Nandula, V. K. 2008. Aminomethylphosphonic acid accumulation in plant species treated with glyphosate. J. Agric. Food Chem. 56:2125–2130.Google Scholar
Ribeiro, D. N., Dayan, F. E., Pan, Z., Duke, S. O., Shaw, D. R., Nandula, V. K., and Baldwin, B. S. 2011. EPSPS gene amplification inheritance in glyphosate-resistant Amaranthus palmeri from Mississippi. Proc. South. Weed Sci. Soc. 64:137.Google Scholar
Shaner, D. L., Nadler-Hassar, T., Henry, W. B., and Koger, C. H. 2005. A rapid in vivo shikimate accumulation assay with excised leaf discs. Weed Sci. 53:769–774.Google Scholar
Sosnoskie, L. M., Kichler, J. M., Wallace, R. D., and Culpepper, A. S. 2011. Multiple resistance in Palmer Amaranth to glyphosate and pyrithiobac confirmed in Georgia. Weed Sci. 59:321–325.Google Scholar
Steckel, L. E., Main, C. L., Ellis, A. T., and Mueller, T. C. 2008. Palmer amaranth (Amaranthus palmeri) in Tennessee has low level glyphosate resistance. Weed Technol. 22:119–123.Google Scholar