Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-28T04:57:03.506Z Has data issue: false hasContentIssue false
  • English
  • Français

Multiple Resistance to Herbicides from Four Site-of-Action Groups in Waterhemp (Amaranthus tuberculatus)

Published online by Cambridge University Press:  20 January 2017

Michael S. Bell ,
Aaron G. Hager  and
Patrick J. Tranel
Michael S. Bell
Affiliation:
Department of Crop Sciences, University of Illinois at Urbana–Champaign, Urbana, IL 61801
Aaron G. Hager
Affiliation:
Department of Crop Sciences, University of Illinois at Urbana–Champaign, Urbana, IL 61801
Patrick J. Tranel*
Affiliation:
Department of Crop Sciences, University of Illinois at Urbana–Champaign, Urbana, IL 61801
*
Corresponding author's E-mail: tranel@illinois.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

In 2006 and 2007, farmers from two counties in Illinois reported failure to control waterhemp with glyphosate. Subsequent onsite field experiments revealed that the populations might be resistant to multiple herbicides. Greenhouse experiments therefore were conducted to confirm glyphosate resistance, and to test for multiple resistance to other herbicides, including atrazine, acifluorfen, lactofen, and imazamox. In glyphosate dose-response experiments, both populations responded similarly to a previously characterized glyphosate-resistant population (MO1). Both Illinois populations also demonstrated high frequencies of resistance to the acetolactate synthase (ALS) inhibitor, imazamox. Additionally, one of the populations demonstrated high frequencies of resistance to both atrazine and the protoporphyrinogen oxidase (PPO) inhibitor, lactofen. Furthermore, using combinations of sequential and tank-mix herbicide applications, individual plants resistant to herbicides spanning all four site-of-action groups were identified from one population. Molecular experiments were performed to provide an initial characterization of the resistance mechanisms and to provide confirmation of the presence of multiple resistance traits within the two populations. Both populations contained the W574L ALS mutation and the ΔG210 PPO mutation, previously shown to confer resistance to ALS and PPO inhibitors, respectively. Atrazine resistance in both populations is suspected to be metabolism-based, because a triazine target-site mutation was not identified. A P106S EPSPS mutation, previously reported to confer glyphosate resistance, was identified in one population. This mutation was identified in both resistant and sensitive plants from the population; however, and so more research is needed to determine the glyphosate-resistance mechanism(s). This is the first known case of a weed population in the United States possessing multiple resistance to herbicides from four site-of-action groups.

Keywords

Acifluorfenatrazineglyphosateimazamoximazethapyrlactofencommon waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif AMATUALS resistanceglyphosate resistancemultiple-herbicide resistancePPO resistanceresistance mechanismtriazine resistance
Type
Weed Management
Information
Weed Science , Volume 61 , Issue 3 , September 2013 , pp. 460 - 468
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Baerson, S. R., Rodriguez, D. J., Tran, M., Feng, Y., Biest, N. A., and Dill, G. M. 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265–1275.Google Scholar
Bernards, M. L., Crespo, R. J., Kruger, G. R., Gaussoin, R. E., and Tranel, P. J. 2012. A waterhemp (Amaranthus tuberculatus) population resistant to 2,4-D. Weed Sci. 60:379–384.CrossRefGoogle Scholar
Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.Google Scholar
Foes, M. J., Liu, L., Tranel, P. J., Wax, L. M., and Stoller, E. W. 1998. A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci. 46:514–520.Google Scholar
Gaines, T. A., Zhang, W., Wang, D., Bukun, B., Chisholm, S. T., Shaner, D. L., Nissen, S. J., Patzoldt, W. L., Tranel, P. J., Culpepper, A. S., Grey, T. L., Webster, T. M., Vencill, W. K., Sammons, R. D., Jiang, J., Preston, C., Leach, J. E., and Westra, P. 2010. Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc. Natl. Acad. Sci. U. S. A. 107:1029–1034.Google Scholar
Green, J. M. 1989. Herbicide antagonism at the whole plant level. Weed Technol. 3:217–226.Google Scholar
Hausman, N. E., Singh, S., Tranel, P. J., Riechers, D. E., Kaundun, S. S., Polge, N. D., Thomas, D. A., and Hager, A. G. 2011. Resistance to HPPD-inhibiting herbicides in a population of waterhemp (Amaranthus tuberculatus) from Illinois, United States. Pest Manag. Sci. 67:258–261.Google Scholar
Jasieniuk, M., Ahmad, R., Sherwood, A. M., Firestone, J. L., Perez-Jones, A., Lanini, W. T., Mallory-Smith, C., and Stednick, Z. 2008. Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: distribution, response to glyphosate, and molecular evidence for an altered target enzyme. Weed Sci. 56:496–502.Google Scholar
Knezevic, S. Z., Streibig, J. C., and Ritz, C. 2007. Utilizing R software package for dose-response studies: the concept and data analysis. Weed Technol. 21:840–848.CrossRefGoogle Scholar
Legleiter, T. R. and Bradley, K. W. 2008. Glyphosate and multiple herbicide resistance in common waterhemp (Amaranthus rudis) populations from Missouri. Weed Sci. 56:582–587.CrossRefGoogle Scholar
McMullan, P. M. and Green, J. M. 2011. Identification of a tall waterhemp (Amaranthus tuberculatus) biotype resistant to HPPD-inhibiting herbicides, atrazine, and thifensulfuron in Iowa. Weed Technol. 25:514–518.Google Scholar
Ott, R. L. and Longnecker, M. 2001. An Introduction to Statistical Methods and Data Analysis. 5th ed. Pacific Grove, CA Duxbury. 1152 p.Google Scholar
Patzoldt, W. L., Dixon, B. S., and Tranel, P. J. 2003. Triazine resistance in Amaranthus tuberculatus (Moq.) Sauer that is not site-of-action mediated. Pest Manag. Sci. 59:1134–1142.Google Scholar
Patzoldt, W. L., Hager, A. G., McCormick, J. S., and Tranel, P. J. 2006. A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase. Proc. Natl. Acad. Sci. U. S. A. 103:12329–12334.Google Scholar
Patzoldt, W. L. and Tranel, P. J. 2007. Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Sci. 55:421–428.Google Scholar
Patzoldt, W. L., Tranel, P. J., and Hager, A. G. 2002. Variable herbicide responses among Illinois waterhemp (Amaranthus rudis and A. tuberculatus) populations. Crop Prot. 21:707–712.CrossRefGoogle Scholar
Patzoldt, W. L., Tranel, P. J., and Hager, A. G. 2005. A waterhemp (Amaranthus tuberculatus) biotype with multiple resistance across three herbicide sites of action. Weed Sci. 53:30–36.Google Scholar
Sauer, J. D. 1955. Revision of the dioecious amaranths. Madroño 13:5–46.Google Scholar
Sauer, J. D. 1957. Recent migration and evolution of the dioecious amaranths. Evolution 11:11–31.Google Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9:218–227.Google Scholar
Steckel, L. E. 2007. The dioecious Amaranthus spp.: here to stay. Weed Technol. 21:567–570.Google Scholar
Thinglum, K. A., Riggins, C. W., Davis, A. S., Bradley, K. W., Al-Khatib, K., and Tranel, P. J. 2011. Wide distribution of the waterhemp (Amaranthus tuberculatus) ΔG210 PPX2 mutation, which confers resistance to PPO-inhibiting herbicides. Weed Sci. 59:22–27.Google Scholar
Tranel, P. J., Riggins, C. W., Bell, M. S., and Hager, A. G. 2011. Herbicide resistances in Amaranthus tuberculatus: a call for new options. J. Agric. Food Chem. 59:5808–5812.Google Scholar
Tranel, P. J. and Trucco, F. 2009. 21st-century weed science: a call for Amaranthus genomics. Pp. 149–161 in Stewart, C. N., ed. Weedy and Invasive Plant Genomics. Ames, IA Blackwell.Google Scholar
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci. 50:700–712.Google Scholar
Wakelin, A. M. and Preston, C. 2006. A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432–440.Google Scholar