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Wide Distribution of the Waterhemp (Amaranthus tuberculatus) ΔG210 PPX2 Mutation, which Confers Resistance to PPO-Inhibiting Herbicides

Published online by Cambridge University Press:  20 January 2017

Kate A. Thinglum ,
Chance W. Riggins ,
Adam S. Davis ,
Kevin W. Bradley ,
Kassim Al-Khatib  and
Patrick J. Tranel
Kate A. Thinglum
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Chance W. Riggins
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Adam S. Davis
Affiliation:
Invasive Weed Management Unit, U.S. Department of Agriculture–Agricultural Research Service, Urbana, IL 61801
Kevin W. Bradley
Affiliation:
Division of Plant Sciences, University of Missouri, Columbia, MO 65211
Kassim Al-Khatib
Affiliation:
Department of Agronomy, Kansas State University, Manhattan, KS 66505
Patrick J. Tranel*
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
*
Corresponding author's E-mail: tranel@illinois.edu
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Abstract

Resistance in waterhemp to herbicides that inhibit protoporphyrinogen oxidase (PPO) previously was shown to result from the deletion of a glycine codon at position 210 (ΔG210) in the PPO-encoding gene, PPX2. Research was conducted to determine if this same mechanism accounted for resistance in geographically separated populations—from Illinois, Kansas, and Missouri—and, if so, to determine if the mutation conferring resistance was independently selected. A dose–response study with lactofen indicated that the resistant populations had different levels of resistance. These differences, however, could be accounted for by different frequencies of resistant individuals within populations and, therefore, the dose–response data were consistent with the hypothesis that the populations contained the same resistance mechanism. Direct evidence in support of this hypothesis was provided by DNA sequencing, which showed that nearly all resistant plants evaluated contained the ΔG210 mutation. A variable region of the PPX2 gene was sequenced and resulting sequences were aligned and organized into a phylogenetic tree. The phylogenetic tree did not reveal clear clustering by either geography or phenotype (resistant vs. sensitive). Possibly recombination within the PPX2 gene has masked its evolutionary history.

Keywords

Common waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif AMATUGene flowherbicide resistancepopulation geneticsmutationevolution
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 59 , Issue 1 , March 2011 , pp. 22 - 27
Copyright
Copyright © Weed Science Society of America 

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