Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-27T10:31:42.904Z Has data issue: false hasContentIssue false
  • English
  • Français

Adjuvant-Increased Glyphosate Uptake by Protoplasts Isolated from Quackgrass Elytrigia repens (L.) Nevski

Published online by Cambridge University Press:  12 June 2017

Hans De Ruiter  and
Esther Meinen
Hans De Ruiter
Affiliation:
DLO Research Institute for Agrobiology and Soil Fertility, P.O. Box 14, 6700 AA Wageningen, The Netherlands
Esther Meinen
Affiliation:
DLO Research Institute for Agrobiology and Soil Fertility, P.O. Box 14, 6700 AA Wageningen, The Netherlands
Get access Rights & Permissions [Opens in a new window]

Abstract

Protoplasts were isolated from quackgrass to investigate the influence of two surfactants and ammonium sulfate on glyphosate uptake. Without adjuvants, the uptake of glyphosate was not detectable. The uptake of the reference compound 2-aminoisobutyric acid was linear with time and was inhibited 60% in the presence of 10 μM carbonyl cyanide m-chlorophenylhydrazone. Glyphosate uptake increased in the presence of both surfactants and ammonium sulfate. After 0.5 h of incubation the internal glyphosate concentration was 37% of the external concentration (100 μM) in the presence of 0.025 g L−1 Ethomeen T/25, 21% in the presence of 0.25 g L−1 Atplus 201, 13% with 5 mM ammonium sulfate, and 20% with 50 mM ammonium sulfate. The surfactant treatments did not reduce either the viability or the internal pH (= 7.1) as judged by fluorescein diacetate staining or 5,5-dimethyloxazolidine-2,4-dione distribution, respectively. The treatments with ammonium sulfate slightly increased the internal pH. The concentration of Ethomeen T/25 required to disrupt the protoplasts was 0.075 g L−1; Atplus 201 did not disrupt the protoplasts at concentrations up to 10 g L−1.

Keywords

Glyphosate, N-(phosphonomethylglycine)Atplus 201 (polyoxyethylene (20) hexitan monolaurate ester)Ethomeen T/25 (polyoxyethylene (15) tallow amine)quackgrass, Elytrigia repens (L.) Nevski ♯ AGRRESurfactantammonium sulfate2-aminoisobutyric acidcell membrane
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 44 , Issue 1 , March 1996 , pp. 38 - 45
Copyright
Copyright © 1996 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Anonymous. 1991. Industrial Surfactants Fatty Amines. Product Bulletin, Akzo Chemicals bv.Google Scholar
2. Bentrup, F. W. and Hoffmann, B. 1992. Amino acid transport across the higher plant cell membrane. Pages 139–151 in Mengel, K. and Pilbeam, D. J., eds. Nitrogen Metabolism of Plants. Proc. Phytochem. Soc. Eur. 33, Phytochemical Society of Europe, Oxford University Press, New York.CrossRefGoogle Scholar
3. Borstlap, A. C., Kuyper-Vos, A. A., and Schuurmans, J. 1987. Transport of 2-aminoisobutyric acid in mesophyll protoplasts of Pisum sativum L. Application of silicone oil layer centrifugation. Plant Sci. 48: 55–62.Google Scholar
4. Bromilow, R. H., Chamberlain, K., Tench, A. J., and Williams, R. H. 1993. Phloem translocation of strong acids—glyphosate, substituted phosphonic and sulfonic acids—in Ricinus communis L. Pestic. Sci. 37: 39–47.CrossRefGoogle Scholar
5. Burton, J. D. and Balke, N. E. 1988. Glyphosate uptake by suspension-cultured potato (Solanum tuberosum and S. brevidens) cells. Weed Sci. 36: 146–153.CrossRefGoogle Scholar
6. Coupland, D., Zabkiewicz, J. A., and Ede, F. J. 1989. Evaluation of three techniques used to determine surfactant phytotoxicity. Ann. Appl. Biol. 115: 147–156.CrossRefGoogle Scholar
7. Denis, M. H. and Delrot, S. 1993. Carrier-mediated uptake of glyphosate in broad bean (Vicia faba) via a phosphate transporter. Physiol. Plant. 87: 569–575.CrossRefGoogle Scholar
8. De Ruiter, H., Verbeek, M. A. M., and Uffing, A. J. M. 1988. Mode of action of a nonionic and a cationic surfactant in relation to glyphosate. Pages 44–55 in Cross, B. and Scher, H. B., eds. Pesticide Formulations, Innovations and Developments. ACS Symposium Series 371, American Chemical Society, Washington DC.CrossRefGoogle Scholar
9. De Ruiter, H., Uffing, A. J. M., Meinen, E., and Prins, A. 1990. Influence of surfactants and plant species on leaf retention of spray solutions. Weed Sci. 38: 567–572.CrossRefGoogle Scholar
10. De Ruiter, H., Verbeek, M. A. M., and Uffing, A. J. M. 1994. Influence of ammonium sulfate and two surfactants on the phytotoxicity and uptake of glyphosate. Meded. Fac. Landbouwwet. Rijksuniv. Gent 59-3B: 1403–1408.Google Scholar
11. Dewey, S.A. and Appleby, A. P. 1983. A comparison between glyphosate and assimilate translocation patterns in tall morning glory (Ipomoea purpurea). Weed Sci. 31: 308–314.CrossRefGoogle Scholar
12. El Ibaoui, H., Delrot, S., Besson, J., and Bonnemain, J. L. 1986. Uptake and release of a phloem-mobile (glyphosate) and of a non-phloem-mobile (iprodione) xenobiotic by broadbean leaf tissues. Physiol. Veg. 24: 431–442.Google Scholar
13. Gaskin, R. E. and Holloway, P. J. 1992. Some physicochemical factors influencing foliar uptake enhancement of glyphosate-mono(isopropylammonium) by polyoxyethylene surfactants. Pestic. Sci. 34: 195–206.CrossRefGoogle Scholar
14. Gougler, J. A. and Geiger, D. R. 1981. Uptake and distribution of N-phosphonomethylglycine in sugar beet plants. Plant Physiol. 68: 668–672.CrossRefGoogle ScholarPubMed
15. Haderlie, L. C., Widholm, J. M., and Slife, F. W. 1977. Effect of glyphosate on carrot and tobacco cells. Plant Physiol. 60: 40–43.CrossRefGoogle ScholarPubMed
16. Helenius, A. and Simons, K. 1975. Solubilization of membranes by detergents. Biochim. Biophys. Acta: 415: 29–79.CrossRefGoogle ScholarPubMed
17. Kasay, F. and Bayer, D. E. 1991. Relationship between intracellular pH and 2,4-D absorption by corn root protoplasts under the influence of metabolic inhibitors and antiauxins. J. Pestic. Sci. 16: 163–170.Google Scholar
18. Larkin, P.J. 1976. Purification and viability determinations of plant protoplasts. Planta 128: 213–216.CrossRefGoogle ScholarPubMed
19. Li, Z-C. and Bush, D. R. 1992. Structural determinants in substrate recognition by proton-amino acid symports in plasma membrane vesicles isolated from sugar beet leaves. Arch. Biochem. Biophys. 294: 519–26.CrossRefGoogle ScholarPubMed
20. Lund-Høie, K. 1979. The physiological fate of glyphosate-14C in Betula verrucosa and Fraxinus excelsior . The effect of ammonium sulfate and the environment on the herbicide. Meldinger fra Norges Landbrukshøgskole, 58: 1–24.Google Scholar
21. Martin, R. A. and Edgington, L. V. 1981 Comparative systemic translocation of several xenobiotics and sucrose. Pestic. Biochem. Physiol. 16: 87–96.CrossRefGoogle Scholar
22. Matsui, H., Shafer, W. E., and Bukovac, M. J. 1992. Surfactant-induced ethylene evolution and pigment efflux from beet (Beta vulgaris L.) root tissue. Pages 59–76 in Foy, C. L., ed., Adjuvants for Agrichemicals. CRC Press. Boca Raton, FL.Google Scholar
23. Mervosh, T. L. and Balke, N. E. 1991. Effects of calcium, magnesium, and phosphate on glyphosate absorption by cultured plant cells. Weed Sci. 39: 347–353.CrossRefGoogle Scholar
24. Nafziger, E. D., Widholm, J. M., and Slife, F. W. 1983. Effects of aspartate and other compounds on glyphosate uptake and growth inhibition in cultured carrot cells Plant Physiol. 71: 623–626.Google Scholar
25. Richard, E. P. and Slife, F. W. 1979 In vivo and in vitro characterization of the foliar entry of glyphosate in hemp dogbane (Apocynum cannabinum). Weed Sci. 27: 426–433.CrossRefGoogle Scholar
26. Riechers, D. E., Wax, D. E., Liebl, R. A., and Bush, D. R. 1994. Surfactant increased glyphosate uptake into plasma membrane vesicles isolated from common lambsquarters leaves. Plant Physiol. 105: 1419–1425.CrossRefGoogle ScholarPubMed
27. Royneberg, T., Balke, N. E., and Lund-Høie, K. 1992. Effects of adjuvants and temperature on glyphosate absorption by cultured cell of velvetleaf (Abutilon theophrasti Medic.). Weed Res. 32: 419–428.CrossRefGoogle Scholar
28. Rubinstein, B. and Tattar, T. A. 1980. Regulation of amino acid uptake into oat mesophyll cells: A comparison between protoplasts and leaf segments. J. Exp. Bot. 31: 269–279.CrossRefGoogle Scholar
29. Sherrick, S. L., Holt, H. A., and Hess, F. D. 1986. Absorption and translocation of MON 0818 adjuvant in field bindweed (Convolvulus arvensis). Weed Sci. 34: 817–823.CrossRefGoogle Scholar
30. Silcox, D. and Holloway, P. J. 1986. The use of potassium leakage to assess potential phytotoxic effects of surfactants. Aspects of Applied Biology 11: 149–158.Google Scholar
31. Steiner, A. A. 1984. Pages 633–650 in The universal nutrient solution. ISOSC Proc. 6th Int. Congress on Soilless Culture.Google Scholar
32. Sterling, T. M. 1994. Mechanisms of herbicide absorption across plant membranes and accumulation in plant cells. Weed Sci. 42: 263–276.CrossRefGoogle Scholar
33. St. John, J. B., Bartels, P. G., and Hilton, J. L. 1974. Surfactant effects on isolated plant cells. Weed Sci. 22: 233–237.CrossRefGoogle Scholar
34. Sutton, D. L. and Foy, C. L. 1971. Effect of diquat and several surfactants on membrane permeability in red beet tissue. Bot. Gaz. 132: 299–304.CrossRefGoogle Scholar
35. Turner, D. J. and Loader, M.P.C. 1980. Effect of ammonium sulfate and other additives upon the phytotoxicity of glyphosate to Agropyron repens (L.) Beauv. Weed Res. 20: 139–146.CrossRefGoogle Scholar
36. Turner, D. J. 1985. Effects on glyphosate performance of formulation. additives and mixing with other herbicides. Pages 221–240 in Grosbard, E. and Atkinson, D., eds. The Herbicide Glyphosate. Butterworth & Co. Google Scholar
37. Walker, N. A. and Smith, F. A. 1975 Intracellular pH in Chora corallina measured by DMO distribution. Plant Sci. Lett. 4: 125–132.CrossRefGoogle Scholar
38. Watson, M.C., Bartels, P. G., and Hamilton, K. C. 1980. Action of selected herbicides and Tween 20 on oat (Avena sativa) membranes. Weed Sci. 28: 122–127.CrossRefGoogle Scholar
39. Wyrill, J. B. and Burnside, O. C. 1976. Absorption, translocation, and metabolism of 2,4-D and glyphosate in common milkweed and hemp dogbane. Weed Sci. 24: 557–566.CrossRefGoogle Scholar