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Characterization of a Common Ragweed (Ambrosia artemisiifolia) Population Resistant to ALS- and PPO-Inhibiting Herbicides

Published online by Cambridge University Press:  20 January 2017

Stephanie L. Rousonelos ,
Ryan M. Lee ,
Murilo S. Moreira ,
Mark J. VanGessel  and
Patrick J. Tranel
Stephanie L. Rousonelos
Affiliation:
Department of Crop Sciences, University of Illinois at Urbana–Champaign, Urbana, IL 61801
Ryan M. Lee
Affiliation:
Department of Crop Sciences, University of Illinois at Urbana–Champaign, Urbana, IL 61801
Murilo S. Moreira
Affiliation:
Department of Plant and Soil Sciences, University of Delaware, Georgetown, DE 19947
Mark J. VanGessel
Affiliation:
Department of Plant and Soil Sciences, University of Delaware, Georgetown, DE 19947
Patrick J. Tranel*
Affiliation:
Department of Crop Sciences, University of Illinois at Urbana–Champaign, Urbana, IL 61801
*
Corresponding author's E-mail: tranel@illinois.edu
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Abstract

A population of common ragweed from Delaware was not controlled in the field by herbicides that inhibit acetolactate synthase (ALS) or protoporphyrinogen oxidase (PPO). Research was conducted to ascertain if this population was resistant to these herbicidal modes of action and, if so, to determine the resistance mechanism(s). Resistance was confirmed by dose-response studies on greenhouse-grown plants with multiple ALS- and PPO-inhibiting herbicides. DNA sequence data revealed that resistance to ALS-inhibiting herbicides was due to the previously reported W574L ALS mutation. To assist in determining the mechanism of resistance to PPO-inhibiting herbicides, an F2 population was derived from a cross between the resistant biotype (Del-R) and a sensitive biotype (DV1-S). This population segregated in the ratio of three resistant : one sensitive when treated with fomesafen, indicating that resistance to PPO-inhibiting herbicides was conferred by a single, (incompletely) dominant, nuclear gene. Sequences of the target-site genes, PPX1 and PPX2, for PPO-inhibiting herbicides were obtained through the screening of a common ragweed cDNA library and subsequent cDNA extension (5′-RACE). Molecular marker analysis with the F2 population revealed that the PPX2 gene cosegregated with resistance to PPO-inhibiting herbicides. A mutation substituting an arginine codon for a leucine codon at a conserved location (R98L) of the PPX2 gene was suspected of being responsible for resistance. By using a transgenic Escherichia coli system, it was demonstrated that the R98L mutation was sufficient to confer resistance to PPO-inhibiting herbicides. The level of resistance to acifluorfen conferred by the R98L mutation in the E. coli system was about 31-fold, similar to the level of resistance seen in the whole-plant dose-response study. Last, a DNA-based assay was developed to identify the presence or absence of the common ragweed PPX2 R98L mutation. The R98L PPX2 mutation is the second mechanism identified for evolved resistance to PPO-inhibiting herbicides.

Keywords

Common ragweed, Ambrosia artemisiifolia L. AMBELAcetolactate synthasecommon ragweedcross-resistanceherbicide resistancemultiple resistanceprotoporphyrinogen oxidaseresistance mechanism
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 60 , Issue 3 , September 2012 , pp. 335 - 344
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403–410.CrossRefGoogle ScholarPubMed
Chikoye, D., Weise, S. F., and Swanton, C. J. 1995. Influence of common ragweed (Ambrosia artemisiifolia) time of emergence and density on white bean (Phaseolus vulgaris). Weed Sci. 43:375–380.Google Scholar
Clay, S. A., Kreutner, B., Clay, D. E., Reese, C., Kleinjan, J., and Forcella, F. 2006. Spatial distribution, temporal stability, and yield loss estimates for annual grasses and common ragweed (Ambrosia artemisiifolia) in a corn/soybean production field over nine years. Weed Sci. 54:380–390.Google Scholar
Clewis, S. B., Askew, S. D., and Wilcut, J. W. 2001. Common ragweed interference in peanut. Weed Sci. 49:768–772.Google Scholar
Coble, H. D., Williams, F. M., and Ritter, R. L. 1981. Common ragweed (Ambrosia artemisiifolia) interference in soybeans (Glycine max). Weed Sci. 29:339–342.Google Scholar
Cowbrough, M. J., Brown, R. B., and Tardif, F. J. 2003. Impact of common ragweed (Ambrosia artemisiifolia) aggregation on economic thresholds in soybean. Weed Sci. 51:947–954.Google Scholar
Dayan, F. E., Daga, P. R., Duke, S. O., Lee, R. M., Tranel, P. J., and Doerksen, R. J. 2010. Biochemical and structural consequences of a glycine deletion in the α-8 helix of protoporphyrinogen oxidase. Biochim. Biophys. Acta. 1804:1548–1556.CrossRefGoogle ScholarPubMed
Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus. 12:13–15.Google Scholar
Friedman, J. and Barrett, S.C.H. 2008. High outcrossing in the annual colonizing species Ambrosia artemisiifolia (Asteraceae). Ann. Bot.-London. 101:1303–1309.Google Scholar
Gressel, J. and Levy, A. 2006. Agriculture: the selector of improbable mutations. Proc. Natl. Acad. Sci. U.S.A. 103:12215–12216.Google Scholar
Heap, I. M. 2011. The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed: August 3, 2011.Google Scholar
Heinemann, I. U., Diekmann, N., Masoumi, A., Koch, M., Messerschmidt, A., Jahn, M., and Jahn, D. 2007. Functional definition of tobacco protoporphyrinogen IX oxidase substrate-binding site. Biochem. J. 402:575–580.Google Scholar
Jung, S., Lee, H. J., Lee, Y., Kang, K., Kim, Y. S., Grimm, B., and Back, K. 2008. Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants. Plant Mol. Biol. 67:535–546.Google Scholar
Knezevic, S. Z., Streibig, J. C., and Ritz, C. 2007. Utilizing R software package for dose-response studies: the concept and data analysis. Weed Technol. 21:840–848.Google Scholar
Lee, R. M., Hager, A. G., and Tranel, P. J. 2008. Prevalence of a novel resistance mechanism to PPO-inhibiting herbicides in waterhemp (Amaranthus tuberculatus). Weed Sci. 56:371–375.CrossRefGoogle Scholar
Moreira, M. S., Johnson, Q. R., Scott, B. A., and VanGessel, M. J. 2006. Cross- and multiple-resistant common ragweed biotype to ALS- and PPO-inhibiting herbicides. Weed Sci. Soc. Am. Abstr. 46:36.Google Scholar
Nishimura, K., Nakayashiki, T., and Inokuchi, H. 1995. Cloning and identification of the hemG gene encoding protoporphyrinogen oxidase (PPO) of Escherichia coli K-12. DNA Res. 2:1–8.CrossRefGoogle ScholarPubMed
Patzoldt, W. L., Hager, A. G., McCormick, J. S., and Tranel, P. J. 2006. A single codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase. Proc. Natl. Acad. Sci. U.S.A. 103:12329–12334.Google Scholar
Patzoldt, W. L., Tranel, P. J., Alexander, A. L., and Schmitzer, P. R. 2001. A common ragweed population resistant to cloransulam-methyl. Weed Sci. 49:485–490.Google Scholar
Ralph, S. A., Foth, B. J., Hall, N., and McFadden, G. I. 2004. Evolutionary pressures on apicoplast transit peptides. Mol. Biol. Evol. 21(12):2183–2194.Google Scholar
Schultz, M. E., Schmitzer, P. R., Alexander, A. L., and Dorich, R. A. 2000. Identification and management of resistance to ALS-inhibiting herbicides in giant ragweed (Ambrosia trifida) and common ragweed (Ambrosia artemisiifolia). Weed Sci. Soc. Am. Abstr. 40:42.Google Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose response relationship. Weed Technol. 9:218–227.Google Scholar
Taylor, J. B., Loux, M. M., Harrison, S. K., and Regnier, E. 2002. Response of ALS-resistant common ragweed (Ambrosia artemisiifolia) and giant ragweed (Ambrosia trifida) to ALS-inhibiting and alternative herbicides. Weed Technol. 16:815–825.Google Scholar
Thinglum, K. A., Riggins, C. W., Davis, A. S., Bradley, K. W., Al-Khatib, K., and Tranel, P. J. 2011. Wide distribution of the waterhemp (Amaranthus tuberculatus) ΔG210 PPX2 mutation, which confers resistance to PPO-inhibiting herbicides. Weed Sci. 59:22–27.Google Scholar
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned. Weed Sci. 50:700–712.CrossRefGoogle Scholar
Watanabe, N., Che, F., Iwano, M., Takayama, S., Yoshida, S., and Isogai, A. 2001. Dual targeting of spinach protoporphyrinogen oxidase II to mitochondria and chloroplasts by alternative use of two in-frame initiation codons. J. Biol. Chem. 276:20474–20481.Google Scholar
Weaver, S. E. 2001. Impact of lamb's-quarters, common ragweed and green foxtail on yield of corn and soybean in Ontario. Can. J. Plant Sci. 81:821–828.Google Scholar
Zheng, D., Patzoldt, W. L., and Tranel, P. J. 2005. Association of the W574L ALS substitution with resistance to cloransulam and imazamox in common ragweed (Ambrosia artemisiifolia). Weed Sci. 53:424–430.Google Scholar
Zybailov, B., Rutschow, H., Friso, G., Rudella, A., Emanuelsson, O., Sun, Q., and van Wijk, K. J. 2008. Sorting signals, N-terminal modifications and abundance of the chloroplast proteome. PLoS ONE. 3(4):e1994 DOI:10.1371/journal.pone.0001994.CrossRefGoogle ScholarPubMed