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Characterization of a norflurazon-resistant mutant of Chlamydomonas reinhardtii

Published online by Cambridge University Press:  12 June 2017

Varsha Vartak  and
Sujata Bhargava
Varsha Vartak
Affiliation:
Department of Botany, University of Pune, Pune 411 007, India
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Abstract

A norflurazon-resistant mutant has been isolated from Chlamydomonas reinhardtii that showed a three-fold factor of resistance over wild type cultures. In comparison to wild type cultures, the mutant showed better retention of chlorophylls and carotenoids when grown in light in the presence of norflurazon. When grown in the dark, chlorophyll losses were similar, while carotenoid losses were lower than in the wild type cultures. Higher levels of phytoene accumulated in the wild type cultures in the presence of norflurazon than in the resistant cultures. The resistant cultures also showed cross tolerance to EMD-IT 5914, a herbicide with a similar mode of action. Norflurazon resistance in this alga appears to arise from alterations in the target enzyme phytoene desaturase.

Keywords

Norflurazon, 4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3(2H)-pyridazinoneEMD-IT 5914, 5-(dimethyl-aminomethylene)-2-oxo-4-phenyl-2,5-dihydrofurane-carbonitrile-(3)Carotenoidsphytoene desaturaseEMD-IT 5914
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 45 , Issue 3 , June 1997 , pp. 374 - 377
Copyright
Copyright © 1997 by the Weed Science Society of America 

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