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Characterization of Atrazine Accumulation by Excised Velvetleaf (Abutilon theophrasti) Roots

Published online by Cambridge University Press:  12 June 2017

Thomas P. Price  and
Nelson E. Balke
Thomas P. Price
Affiliation:
Dep. Agron. Univ. of Wisconsin, Madison, WI 53706
Nelson E. Balke
Affiliation:
Dep. Agron. Univ. of Wisconsin, Madison, WI 53706
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Abstract

Long-term absorption of 14C-atrazine [2-chloro-4 - (ethylamino) - 6 - (isopropylamino) - s - triazine] by excised velvetleaf (Abutilon theophrasti Medic.) roots was investigated. A rapid, initial phase of absorption was followed by a long-term, linear phase of absorption, which resulted in atrazine accumulation in the tissue to concentrations above the external solution. This accumulation did not result from metabolism of the atrazine. Aging of the excised tissue appeared to be responsible for the accumulation. Dinitrophenol, but not cyanide, inhibited accumulation. Atrazine accumulation decreased as the temperature during absorption was decreased. The Q10 the absorption rate was ca. 2.5 between 15 and 25 C. Efflux of atrazine from the tissue occurred in two phases. At 25 C, loss of 80% of the atrazine occurred in 20 min. At 4C, the rate of this efflux was slower and the phase lasted longer. At both 4 and 25 C a second, slower phase of efflux occurred through at least 6 h and resulted in loss of equal amounts of atrazine at the two temperatures. These experiments show that, in addition to rapid absorption of atrazine to a passive equilibrium, excised velvetleaf roots accumulate atrazine by a metabolically dependent process over long periods of time.

Keywords

Herbicide absorptionherbicide effluxmetabolic inhibitorsherbicide metabolismtissue aging
Type
Research Article
Information
Weed Science , Volume 31 , Issue 1 , January 1983 , pp. 14 - 19
Copyright
Copyright © 1983 Weed Science Society of America 

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References

Literature Cited

1. Ashton, F. M. and Crafts, A. S. 1981. Mode of Action of Herbicides, Second edition. Wiley - Interscience, New York. 525.Google Scholar
2. Dhillon, P. S., Byrnes, W. R., and Merritt, C. 1968. Simazine distribution and degradation in red pine seedlings. Weed Sci. 16:374–376.Google Scholar
3. Ebert, E. and Dumford, S. W. 1976. Effects of triazine herbicides on the physiology of plants. Residue Rev. 65:1–103.Google Scholar
4. Epstein, E. 1972. Mineral Nutrition of Plants: Principles and Perspectives. John Wiley and Sons, Inc., New York. 412.Google Scholar
5. Geller, A. 1979. Sorption and desorption of atrazine by three bacterial species isolated from aquatic systems. Arch. Environ. Contam. Toxicol. 8:713–720.Google Scholar
6. Gronewald, J. W., Cheeseman, J. M., and Hanson, J. B. 1979. Comparison of the responses of corn root tissue to fusiccocin and washing. Plant Physiol. 63:255–259.Google Scholar
7. Hodges, T. K. 1973. Ion absorption by plant roots. Adv. Agron. 25:163–207.Google Scholar
8. Lawrence, J. F. 1974. Comparison of extraction methods of triazine herbicides in root crops using electrolytic conductivity detection. J. Agric. Food Chem. 22:137–140.Google Scholar
9. Leonard, R. T. and Hanson, J. B. 1972. Induction and development of increased ion absorption in corn root tissue. Plant Physiol. 49:430–435.Google Scholar
10. Leonard, R. T. and Hanson, J. B. 1972. Increased membrane-bound*** adenosine triphosphatase activity accompanying development of enhanced solute uptake in washed corn tissue. Plant Physiol. 49:436–440.Google Scholar
11. Moody, K., Kust, C. A., and Buchholtz, K. P. 1970. Release of herbicides by soybean roots in culture solutions. Weed Sci. 18: 214–218.Google Scholar
12. Moody, K., Kust, C. A., and Buchholtz, K. P. 1970. Uptake of herbicides by soybean roots in culture solutions. Weed Sci. 18: 642–647.CrossRefGoogle Scholar
13. Moreland, D. E. 1980. Mechanisms of action of herbicides. Annu. Rev. Plant Physiol. 31:597–638.Google Scholar
14. Orwick, P. L., Schreiber, M. M., and Hodges, T. K. 1976. Absorption and efflux of chloro-s-triazines by Setaria roots. Weed Res. 16:139–144.Google Scholar
15. Peterson, C.A. and Edgington, L. V. 1976. Entry of pesticides into the plant symplast as measured by their loss from an ambient solution. Pestic. Sci. 7:483–491.Google Scholar
16. Price, T. P. and Balke, N. E. 1982. Characterization of rapid atrazine absorption by excised velvetleaf (Abutilon theophrasti) roots. Weed Sci. 30: (In press).Google Scholar
17. Schonbaum, G. R., Bonner, W. D. Jr., Storey, B. T., and Bahr, J. T. 1971. Specific inhibition of the cyanide - insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 47:124–128.Google Scholar
18. Sheets, T. J. 1961. Uptake and distribution of simazine by oat and cotton seedlings. Weeds 9:1–13.Google Scholar
19. Shimabukuro, R. H., Kadunce, R. E., and Frear, D. S. 1966. Dealkylation of atrazine in mature pea plants. J. Agric. Food Chem. 14:392–395.Google Scholar
20. Shimabukuro, R. H., Masteller, V. J., and Walsh, W. C. 1976. Atrazine injury: relationship to metabolism, substrate level, and secondary factors. Weed Sci. 24:336–340.CrossRefGoogle Scholar
21. Shone, M.G.T., Clarkson, D. T., Sanderson, J., and Wood, A. V. 1973. A comparison of the uptake and translocation of some organic molecules and ions in higher plants. Pages 571–582 in Anderson, W. P., ed. Ion Transport in Plants. Academic Press, New York.Google Scholar
22. Solomos, T. 1977. Cyanide - resistant respiration in higher plants. Annu. Rev. Plant. Physiol. 28:279–297.CrossRefGoogle Scholar
23. Van Stevenick, R.F.M. 1975. The “washing” or “aging” phenomenon in plant tissues. Annu. Rev. Plant Physiol. 26:237–258.Google Scholar
24. Vostral, H. J., Buchholtz, K. P., and Kust, C. A. 1970. Effects of root temperature on absorption and translocation of atrazine in soybeans. Weed Sci. 18:115–117.Google Scholar
25. Ward, M. T. and Weber, J. B. 1968. Aqueous solubility of alkylamino-s-triazines as a function of pH and molecular structure. J. Agric. Food Chem. 16:959–961.Google Scholar
26. Weete, J. D. 1977. Herbicide analysis by chromatographic techniques. Pages 97–108 in Truelove, B., ed. Research Methods in Weed Science, Second edition. South. Weed Sci. Soc. Auburn Printing, Auburn, AL.Google Scholar