Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-10T07:45:27.871Z Has data issue: false hasContentIssue false
  • English
  • Français

Cloning and Expression Analysis of Alpha-Tubulin Genes in Water Foxtail (Alopecurus aequalis)

Published online by Cambridge University Press:  20 January 2017

Saima Hashim ,
Mayumi Hachinohe  and
Hiroshi Matsumoto
Saima Hashim
Affiliation:
Doctoral Program in Life Sciences and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan
Mayumi Hachinohe
Affiliation:
Doctoral Program in Life Sciences and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan
Hiroshi Matsumoto*
Affiliation:
Doctoral Program in Life Sciences and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan
*
Corresponding author's E-mail: hmatsu@biol.tsukuba.ac.jp
Get access Rights & Permissions [Opens in a new window]

Abstract

Tubulins are encoded by small gene families in plants. Here we report the cloning and characterization of water foxtail α-tubulin genes (AaTUA), an economically important weed. The genome of water foxtail contains TUA family consisting of at least four genes. Using degenerate primers, four partial TUA genes were isolated from the complementary DNA (cDNA) of water foxtail. Using the partial gene sequences, specific primers for each TUA gene were designed and full-length TUA cDNAs were isolated. These genes were designated as AaTUA1 to AaTUA4. The deduced amino acid sequences of AaTUA genes showed significant homology to the TUA genes of barley, corn, and Arabidopsis. The coding sequences of the AaTUA1 and AaTUA3 genes were interrupted by three introns and there were four introns in the coding regions of AaTUA2 and AaTUA4. The organ-specific expression of AaTUA genes showed that AaTUA1 and AaTUA4 were predominantly expressed in all organs examined (root, stem, young leaf, mature leaf, and panicle), whereas AaTUA2 was expressed mainly in roots and AaTUA3 was expressed in stem, root, and panicle. Abscisic acid (ABA) and gibberellic acid (GA) differentially induced the expression of AaTUA1 and AaTUA3. Moreover, trifluralin, propham, and caffeic acid induced the expression of all AaTUA genes in a dose-dependent manner except AaTUA2. This is the first report of complete sequences of AaTUA genes and the first characterization of these genes for any Alopecurus species in the literature.

Keywords

Water foxtail, Alopecurus aequalis SobolArabidopsis, Arabidopsis thaliana L.barley, Hordeum vulgare Lcorn, Zea mays L.Genegene expressionmRNAintronsexons
Type
Research Article
Information
Weed Science , Volume 58 , Issue 2 , June 2010 , pp. 89 - 95
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Abdrakhamanova, A., Wang, Q. Y., Khokhlova, L., and Nick, P. 2003. Is microtubule disassembly a trigger for cold acclimation? Plant Cell. Physiol. 44:676–686.Google Scholar
Anthony, R. G., Reichelt, S., and Hussey, P. J. 1999. Dinitroaniline herbicide-resistant transgenic tobacco plants generated by co-overexpression of a mutant α-tubulin and a β-tubulin. Nat. Biotechnol. 17:712–716.Google Scholar
Bartels, P. G. and Hilton, J. L. 1973. Comparison of trifluralin, oryzalin, pronamide, propham and colchicine treatments on microtubules. Pestic. Biochem. Physiol. 3:462–472.CrossRefGoogle Scholar
Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159.Google Scholar
Cope, T. A. 1982. Poaceae. Pages 678. In Nasir, E. and Ali, S. I. Flora of Pakistan. Fascicle No. 143. Islamabad: National Herbarium, and Karachi, Pakistan: Botany Department, University of Karachi.Google Scholar
Coss, R. A. and Pickett-Heaps, J. D. 1974. The effects of isopropyl N-phenylcarbamate on the green alga Oedogoniun cardiacum . J. Cell Biol. 63:84–98.Google Scholar
Farajalla, M. R. and Gulick, P. J. 2007. The α-tubulin gene family in wheat (Triticum aestivum L.) and differential gene expression during cold acclimation. Genome. 50:502–510.CrossRefGoogle Scholar
Gianì, S. and Breviario, D. 1996. Rice β-tubulins mRNA levels are modulated during flower development and in response to external stimuli. Plant Sci. 116:147–157.Google Scholar
Gianì, S., Qin, X., Faoro, F., and Breviario, D. 1998. In rice, Oryzalin and abscisic acid differentially affect tubulin mRNA and protein levels. Planta. 205:334–341.Google ScholarPubMed
Gulick, P. J., Drouin, S., Yu, Z., Danyluk, J., Poisson, G., Monroy, A. F., and Sarhan, F. 2005. Transcriptome comparison of winter and spring wheat responding to low temperature. Genome. 48:913–923.Google Scholar
Huang, R. F. and Lloyd, C. W. 1999. Gibberellic acid stabilizes microtubules in maize suspension cells to cold and stimulates acetylation of alpha-tubulin. FEBS Lett. 443:317–320.Google Scholar
Hugdahl, J. D. and Morejohn, L. C. 1993. Rapid and reversible high-affinity binding of the dinitroaniline herbicide Oryzalin to tubulin from Zea mays L. Plant Physiol. 102:725–740.Google Scholar
Jeon, J. S., Lee, S., Jung, K. H., Jun, S. H., Kim, C., and An, G. 2000. Tissue preferential expression of a rice α-tubulin gene, OsTubA1, mediated by the first intron. Plant Physiol. 123:1005–1014.CrossRefGoogle ScholarPubMed
Kopczak, S. D., Haas, N. A., Hussey, P. J., Silflow, C. D., and Snustad, D. P. 1992. The small genome of Arabidopsis contains at least six expressed α-tubulin genes. Plant Cell. 4:539–547.Google ScholarPubMed
Mayer, U. and Jürgens, G. 2002. Microtubule cytoskeleton: a track record. Curr. Opin. Plant Biol. 5:494–501.Google Scholar
Mendu, M. and Silflow, C. D. 1993. Elevated levels of tubulin transcripts accompany the GA3-induced elongation of oat internode segment. Plant Cell Physiol. 34:973–983.Google Scholar
Morita, H. 1997. Handbook of Arable Weeds in Japan. Tokyo Kumiai Chemical Industry. 62.Google Scholar
Paul, D. C. and Goff, C. W. 1973. Comparative effects of caffeine, its analogues, and calcium deficiency on cytokinesis. Exp. Cell Res. 78:399–413.Google Scholar
Qin, X., Gianì, S., and Breviario, D. 1997. Molecular cloning of three rice α-tubulin isotypes: differential expression in tissues and during flower development. Biochim. Biophys. Acta. 1354:19–23.Google Scholar
Radchuk, V., Sreenivasulu, N., Blume, Y., and Weschke, W. 2007. Distinct tubulin genes are differentially expressed during barley grain development. Physiol. Plant. 131:571–580.Google Scholar
Radchuk, V., Sreenivasulu, N., Blume, Y., and Weschke, W. 2008. Cloning and expression of the tubulin genes in barley. Cell Biol. Int. 32:557–559.Google Scholar
Schröder, J., Stenger, H., and Wernicke, W. 2001. α-Tubulin genes are differentially expressed during leaf cell development in barley (Hordeum vulgare L.). Plant Mol. Biol. 45:723–730.CrossRefGoogle ScholarPubMed
Snustad, D. P., Haas, N. A., Kopczak, S. D., and Silflow, C. D. 1992. The small genome of Arabidopsis contains at least 9 expressed beta-tubulin genes. Plant Cell. 4:549–556.Google Scholar
Stotz, H. U. and Long, S. R. 1999. Expression of the pea (Pisum sativum L.) alpha-tubulin gene TubA1 is correlated with cell division activity. Plant Mol. Biol. 41:601–614.Google Scholar
Tardif, G., Kane, N. A., Adam, H., Labrie, L., Major, G., Gulick, P., Sarhan, F., and Laliberté, J. F. 2007. Interaction network of proteins associated with abiotic stress response and development in wheat. Plant Mol. Biol. 63:703–718.Google Scholar
Vaughn, K. C., Marks, M. D., and Weeks, D. P. 1987. A Dinitroaniline-resistant mutant of Eleusine indica exhibits cross-resistance and supersensitivity to antimicrotubule herbicides and drugs. Plant Physiol. 83:956–964.CrossRefGoogle ScholarPubMed
Villemur, R., Haas, N. A., Joyce, C. M., Snustad, D. P., and Silflow, C. D. 1994. Characterization of four new beta-tubulin genes and their expression during male flower development in maize (Zea mays L.). Plant Mol. Biol. 24:295–315.Google Scholar
Villemur, R., Joyce, C. M., Haas, N. A., Goddard, R. H., Kopczak, S. D., Hussey, P. J., Snustad, D. P., and Silflow, C. D. 1992. α-Tubulin gene family of maize (Zea mays L.)—evidence for 2 ancient a-tubulin genes in plants. J. Mol. Biol. 227:81–96.Google Scholar
Yoshikawa, M., Yang, G., Kawaguchi, K., and Komatsu, S. 2003. Expression analyses of beta-tubulin isotype genes in rice. Plant Cell Physiol. 44:1202–1207.Google Scholar