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Comparison of Chlorophyll Fluorescence and Fresh Weight as Herbicide Bioassay Techniques

Published online by Cambridge University Press:  12 June 2017

David R. Shaw ,
Thomas F. Peeper  and
D. L. Nofziger
David R. Shaw
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
Thomas F. Peeper
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
D. L. Nofziger
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
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Abstract

The sensitivities of chlorophyll fluorescence and fresh weight as bioassay techniques for the determination of metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5 (4H)-one], diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], and atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] concentrations in soil were compared. The ratio of the initial inflection point (I) to the variable fluorescence maximum (P) of intact oat (Avena sativa L. ‘Chilocco’) leaves was determined for plants seeded directly into herbicide-treated soil and for those transplanted into treated soil after 14 days of growth in nutrient solution. Using the chlorophyll fluorescence of transplanted oats bioassay, 0-, 0.13-, 0.25-, and 0.50-ppm concentrations could be distinguished from one another within 8 h for metribuzin, within 24 h for diuron, and within 48 h for atrazine. These distinctions between rates could not be made 17 days after seeding into treated soil when using fresh weight as the bioassay indicator. Chlorophyll fluorescence of oats seeded directly into treated soil was also a reliable technique, but required much more time to attain sufficient plant size for convenient chlorophyll fluorescence determinations.

Keywords

Avena sativametribuzin, diuronatrazine
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 33 , Issue 1 , January 1985 , pp. 29 - 33
Copyright
Copyright © 1985 by the Weed Science Society of America 

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References

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